Stable-isotope labeling with amino acids in nematodes
Open accessAbstract
We describe an approach for accurate quantitation of global protein dynamics in Caenorhabditis elegans. We adapted stable-isotope labeling with amino acids in cell culture (SILAC) for nematodes by feeding worms a heavy lysine- and heavy arginine-labeled Escherichia coli strain and report a genetic solution to elminate the problem of arginine-to-proline conversion. Combining our approach with quantitative proteomics methods, we characterized the heat-shock response in worms.
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Near-complete incorporation of heavy lysine into the proteome was achieved by feeding worms with SILAC-labeled E. coli.
“Near-complete incorporation of 13C6-14N2-lysine into the proteome was achieved by feeding worms with SILAC labeled (“heavy”) E. coli cells (Larance et al., 2011).”
From Widespread Proteome Remodeling and Aggregation in Aging C. elegans (2015) · cites this paper as Larance et al., 2011
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- [8]Lamond AI Boisvert FM Lam YW Lamont D Mol. Cell. Proteomics 2010 9 457 470 20026476 PMC2849709
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Widespread Proteome Remodeling and Aggregation in Aging C. elegans
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Deep proteome analysis identifies age-related processes in C. elegans
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- Authors
- Larance, M., Bailly, A. P., Pourkarimi, E., Hay, R. T., Buchanan, G., Coulthurst, S., Xirodimas, D. P., Gartner, A., & Lamond, A. I.
- Affiliations
- University of Dundee (GB)
- Centre National de la Recherche Scientifique (FR)
- Corresponding author
- Gartner, Anton
- Lamond, Angus I
- ORCID
- Venue
- Nature Methods
- Published
- 2011-08-28
- Volume
- 8
- Issue
- 10
- Pages
- 849-851
- ISSN
- 1548-7091 (ISSN-L)
- 1548-7105
- PMC
- PMC3184259
- OpenAlex
- W1976685504
- Cited by
- 120
- References
- 17
- Citations by year
- 2026: 3 · 2025: 1 · 2024: 6 · 2023: 1 · 2022: 5 · 2021: 5 · 2020: 4 · 2019: 6 · 2018: 9 · 2017: 7 · 2016: 7 · 2015: 13 · 2014: 17 · 2013: 18 · 2012: 18
- FWCI
- 7.70
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Nature Methods (publishedVersion)
- PubMed (publishedVersion)
- Discovery Research Portal (University of Dundee) (publishedVersion)
- PubMed Central (acceptedVersion)
- HAL (submittedVersion)
- Scholarworks@UNIST (Ulsan National Institute of Science and Technology) (submittedVersion)
- Funders
- Wellcome Trust (073980, 090944, 0909444/Z/09/Z, 083524/Z/07/Z, 083524, 08136/Z/03/Z, 073980/Z/03/Z)
- Topics
- Physical Sciences › Chemistry › Spectroscopy › Advanced Proteomics Techniques and Applications
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ubiquitin and proteasome pathways
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Aging › Genetics, Aging, and Longevity in Model Organisms
- Keywords
- Caenorhabditis elegans, quantitative proteomics, heat shock response, heat shock proteins, protein quantification
- MeSH terms
- Animals, Arginine, Cells, Cultured, Escherichia coli, Isotope Labeling, Lysine, Proline, Caenorhabditis elegans, Heat-Shock Response, Proteomics
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