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Dual-objective STORM reveals three-dimensional filament organization in the actin cytoskeleton
Open accessAbstract
By combining astigmatism imaging with a dual-objective scheme, we improved the image resolution of stochastic optical reconstruction microscopy (STORM) and obtained <10-nm lateral resolution and <20-nm axial resolution when imaging biological specimens. Using this approach, we resolved individual actin filaments in cells and revealed three-dimensional ultrastructure of the actin cytoskeleton. We observed two vertically separated layers of actin networks with distinct structural organizations in sheet-like cell protrusions.
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Hell, S. W. (2007) · Science · doi:10.1126/science.1137395 · Cited by 2,967
Environmental sensing through focal adhesions
Geiger, B., Spatz, J. P., & Bershadsky, A. D. (2009) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm2593 · Cited by 2,543
Cited by
Actin Dynamics, Architecture, and Mechanics in Cell Motility
Blanchoin, L., Boujemaa-Paterski, R., Sykes, C., & Plastino, J. (2014) · Physiological Reviews · doi:10.1152/physrev.00018.2013 · Cited by 1,457
Super-resolution microscopy with DNA-PAINT
Schnitzbauer, J., Strauss, M. T., Schlichthaerle, T., Schueder, F., & Jungmann, R. (2017) · Nature Protocols · doi:10.1038/nprot.2017.024 · Cited by 1,047
Shim, S.-H., Xia, C., Zhong, G., Babcock, H. P., Vaughan, J. C., Huang, B., Wang, X., Xu, C., Bi, G.-Q., & Zhuang, X. (2012) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1201882109 · Cited by 514
Self-citationOrganization and Function of Membrane Contact Sites
Helle, S. C. J., Kanfer, G., Kolar, K., Lang, A., Michel, A. H., & Kornmann, B. (2013) · Biochimica et Biophysica Acta (BBA) - Molecular Cell Research · doi:10.1016/j.bbamcr.2013.01.028 · Cited by 453
Cell mechanics: principles, practices, and prospects
Moeendarbary, E., & Harris, A. R. (2014) · WIREs Systems Biology and Medicine · doi:10.1002/wsbm.1275 · Cited by 342
Metadata
- Authors
- Xu, K., Babcock, H. P., & Zhuang, X.
- Venue
- Nature Methods
- Published
- 2012-01-08
- Volume
- 9
- Issue
- 2
- Pages
- 185-188
- ISSN
- 1548-7091 (ISSN-L)
- 1548-7105
- OpenAlex
- W2034713721
- Cited by
- 472
- References
- 2
- FWCI
- 21.65
- Type
- journal article
- Language
- en
- Open access
- green
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