Direct observation of mammalian cell growth and size regulation
Open accessAbstract
We introduce a microfluidic system for simultaneously measuring single-cell mass and cell cycle progression over multiple generations. We use this system to obtain over 1,000 h of growth data from mouse lymphoblast and pro-B-cell lymphoid cell lines. Cell lineage analysis revealed a decrease in the growth rate variability at the G1-S phase transition, which suggests the presence of a growth rate threshold for maintaining size homeostasis.
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Cells are measured in suspension under culture conditions with controlled media temperature and carbon dioxide concentration to maintain viability and growth.
“Cells are measured in suspension while under culture conditions, with controlled media temperature and CO2 concentration to maintain cell viability and growth13.”
From Drug sensitivity of single cancer cells is predicted by changes in mass accumulation rate (2016) · cites this paper as [13]
The SMR passes single cells in suspension and produces a frequency shift proportional to cell buoyant mass.
“In short, single cells in suspension are passed through the SMR resulting in a frequency shift that is proportional to cell buoyant mass.”
From Drug sensitivity of single cancer cells is predicted by changes in mass accumulation rate (2016) · cites this paper as [13]
The SMR design and operation were described in earlier work.
“The design and operation of the SMR have been previously described13–15.”
From Drug sensitivity of single cancer cells is predicted by changes in mass accumulation rate (2016) · cites this paper as [13]
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Visualizing Spatiotemporal Dynamics of Multicellular Cell-Cycle Progression
Sakaue-Sawano, A., Kurokawa, H., Morimura, T., Hanyu, A., Hama, H., Osawa, H., Kashiwagi, S., Fukami, K., Miyata, T., Miyoshi, H., et al. (2008) · Cell · doi:10.1016/j.cell.2007.12.033 · Cited by 2,123 · see citing works
Weighing of biomolecules, single cells and single nanoparticles in fluid
Burg, T. P., Godin, M., Knudsen, S. M., Shen, W., Carlson, G., Foster, J. S., Babcock, K., & Manalis, S. R. (2007) · Nature · doi:10.1038/nature05741 · Cited by 1,213 · see citing works
Self-citationSize Control in Animal Development
Conlon, I. J., & RAFF, M. C. (1999) · Cell · doi:10.1016/s0092-8674(00)80563-2 · Cited by 743 · see citing works
How Cells Coordinate Growth and Division
Jorgensen, P., & Tyers, M. (2004) · Current Biology · doi:10.1016/j.cub.2004.11.027 · Cited by 559 · see citing works
Self-citationThe effects of molecular noise and size control on variability in the budding yeast cell cycle
Talia, S. D., Skotheim, J. M., Bean, J. M., Siggia, E. D., & Cross, F. R. (2007) · Nature · doi:10.1038/nature06072 · Cited by 472 · see citing works
Cell Growth and Size Homeostasis in Proliferating Animal Cells
Tzur, A., Kafri, R., LeBleu, V. S., Lahav, G., & Kirschner, M. W. (2009) · Science · doi:10.1126/science.1174294 · Cited by 403 · see citing works
Self-citationUsing buoyant mass to measure the growth of single cells
Godin, M., Delgado, F. F., Son, S., Grover, W. H., Bryan, A. K., Tzur, A., Jorgensen, P., Payer, K., Grossman, A. D., Kirschner, M. W., et al. (2010) · Nature Methods · doi:10.1038/nmeth.1452 · Cited by 394 · see citing works
Self-citationInterference Microscopy and Mass Determination
Davies, H. G., & Wilkins, M. H. F. (1952) · Nature · doi:10.1038/169541a0 · Cited by 212 · see citing works
Conlon, I. J., & RAFF, M. C. (2003) · Journal of Biology · doi:10.1186/1475-4924-2-7 · Cited by 208 · see citing works
Controls over the timing of DNA replication during the cell cycle of fission yeast
Nurse, P., & Thuriaux, P. (1977) · Experimental Cell Research · doi:10.1016/0014-4827(77)90358-5 · Cited by 203 · see citing works
Mitchison, J. M. (2003) · International review of cytology · doi:10.1016/s0074-7696(03)01004-0 · Cited by 168 · see citing works
Extracellular control of cell size
Conlon, I. J., Dunn, G., Mudge, A. W., & RAFF, M. C. (2001) · Nature Cell Biology · doi:10.1038/ncb1001-918 · Cited by 128 · see citing works
Control and maintenance of mammalian cell size
Cooper, S. (2004) · BMC Cell Biology · doi:10.1186/1471-2121-5-35 · Cited by 49 · see citing works
Cell cycle control — both deterministic and probabilistic?
Nurse, P. (1980) · Nature · doi:10.1038/286009a0 · Cited by 39 · see citing works
Size control in growing yeast and mammalian cells.
Sveiczer, Á., Novák, B., & Mitchison, J. M. (2004) · Theoretical Biology and Medical Modelling · doi:10.1186/1742-4682-1-12 · Cited by 18 · see citing works
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Research works printed but not linked: 2
- [16]Burg TP Nature 446 1066 1069 2007 17460669
- [17]Sakaue-Sawano A Cell 132 487 498 2008 18267078
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Taheri-Araghi, S., Bradde, S., Sauls, J. T., Hill, N. S., Levin, P. A., Paulsson, J., Vergassola, M., & Jun, S. (2014) · Current Biology · doi:10.1016/j.cub.2014.12.009 · Cited by 715 · see citing works
On being the right (cell) size
Ginzberg, M., Kafri, R., & Kirschner, M. (2015) · Science · doi:10.1126/science.1245075 · Cited by 390 · see citing works
Self-citationLloyd, A. C. (2013) · Cell · doi:10.1016/j.cell.2013.08.053 · Cited by 393 · see citing works
Mehling, M., & Tay, S. (2013) · Current Opinion in Biotechnology · doi:10.1016/j.copbio.2013.10.005 · Cited by 392 · see citing works
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Byun, S., Son, S., Amodei, D., Cermak, N., Bagnall, J., Kang, J. H., Hecht, V. C., Winslow, M. M., Jacks, T., Mallick, P., et al. (2013) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1218806110 · Cited by 364 · see citing works
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Zielke, N., Korzelius, J., van Straaten, M., Bender, K., Schuhknecht, G. F. P., Dutta, D., Xiang, J., & Edgar, B. A. (2014) · Cell Reports · doi:10.1016/j.celrep.2014.03.020 · Cited by 286 · see citing works
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Kafri, R., Levy, J., Ginzberg, M. B., Oh, S., Lahav, G., & Kirschner, M. W. (2013) · Nature · doi:10.1038/nature11897 · Cited by 232 · see citing works
Self-citationLive-cell mass profiling: an emerging approach in quantitative biophysics
Zangle, T. A., & Teitell, M. A. (2014) · Nature Methods · doi:10.1038/nmeth.3175 · Cited by 260 · see citing works
Spatial constraints control cell proliferation in tissues
Streichan, S. J., Hoerner, C. R., Schneidt, T., Holzer, D., & Hufnagel, L. (2014) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1323016111 · Cited by 246 · see citing works
High-throughput measurement of single-cell growth rates using serial microfluidic mass sensor arrays
Cermak, N., Olcum, S., Delgado, F. F., Wasserman, S. C., Payer, K. R., A Murakami, M., Knudsen, S. M., Kimmerling, R. J., Stevens, M. M., Kikuchi, Y., et al. (2016) · Nature Biotechnology · doi:10.1038/nbt.3666 · Cited by 227 · see citing works
Self-citationSize control in mammalian cells involves modulation of both growth rate and cell cycle duration
Cadart, C., Monnier, S., Grilli, J., Sáez, P. J., Srivastava, N., Attia, R., Terriac, E., Baum, B., Lagomarsino, M. C., & Piel, M. (2018) · Nature Communications · doi:10.1038/s41467-018-05393-0 · Cited by 239 · see citing works
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Jun, S., Si, F., Pugatch, R., & Scott, M. (2018) · Reports on Progress in Physics · doi:10.1088/1361-6633/aaa628 · Cited by 186 · see citing works
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Uroz, M., Wistorf, S., Serra‐Picamal, X., Conte, V., Sales‐Pardo, M., Roca‐Cusachs, P., Guimerà, R., & Trepat, X. (2018) · Nature Cell Biology · doi:10.1038/s41556-018-0107-2 · Cited by 185 · see citing works
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Osella, M., Nugent, E., & Lagomarsino, M. C. (2014) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1313715111 · Cited by 181 · see citing works
Chip in a lab: microfluidics for next generation life science research
Streets, A. M., & Huang, Y. (2013) · Biomicrofluidics · doi:10.1063/1.4789751 · Cited by 180 · see citing works
Etayash, H., Khan, M. F., Kaur, K., & Thundat, T. G. (2016) · Nature Communications · doi:10.1038/ncomms12947 · Cited by 171 · see citing works
Cell-Size ControlPreprint
Amodeo, A. A., & Skotheim, J. M. (2015) · Cold Spring Harbor Perspectives in Biology · doi:10.1101/cshperspect.a019083 · Cited by 166 · see citing works
Resonant microchannel volume and mass measurements show that suspended cells swell during mitosis
Son, S., Kang, J. H., Oh, S., Kirschner, M. W., Mitchison, T. J., & Manalis, S. R. (2015) · The Journal of Cell Biology · doi:10.1083/jcb.201505058 · Cited by 157 · see citing works
Self-citationCellular Allometry of Mitochondrial Functionality Establishes the Optimal Cell Size
Miettinen, T. P., & Björklund, M. (2016) · Developmental Cell · doi:10.1016/j.devcel.2016.09.004 · Cited by 156 · see citing works
FUCCI sensors: powerful new tools for analysis of cell proliferation
Zielke, N., & Edgar, B. A. (2015) · Wiley Interdisciplinary Reviews Developmental Biology · doi:10.1002/wdev.189 · Cited by 145 · see citing works
Nanotechnology: emerging tools for biology and medicinePreprint
Wong, I. Y., Bhatia, S. N., & Toner, M. (2013) · Genes & Development · doi:10.1101/gad.226837.113 · Cited by 150 · see citing works
Jones, A. R., Forero, M. G., Withers, S. P., Smith, R. S., Traas, J. A., Dewitte, W., & Murray, J. A. H. (2017) · Nature Communications · doi:10.1038/ncomms15060 · Cited by 145 · see citing works
The physics of cell-size regulation across timescales
Cadart, C., Venkova, L. S., Recho, P., Lagomarsino, M. C., & Piel, M. (2019) · Nature Physics · doi:10.1038/s41567-019-0629-y · Cited by 146 · see citing works
Mitochondrial Function and Cell Size: An Allometric Relationship
Miettinen, T. P., & Björklund, M. (2017) · Trends in Cell Biology · doi:10.1016/j.tcb.2017.02.006 · Cited by 142 · see citing works
Ginzberg, M. B., Chang, N. W., D'Souza, H., Patel, N., Kafri, R., & Kirschner, M. W. (2018) · eLife · doi:10.7554/elife.26957 · Cited by 127 · see citing works
Self-citationCancer stem cells and cell size: A causal link?
Li, Q., Rycaj, K., Chen, X., & Tang, D. G. (2015) · Seminars in Cancer Biology · doi:10.1016/j.semcancer.2015.07.002 · Cited by 119 · see citing works
Oh, S., Lee, C., Yang, W., Li, A., Mukherjee, A., Basan, M., Ran, C., Yin, W. H., Tabin, C. J., Fu, D., et al. (2022) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2117938119 · Cited by 115 · see citing works
Self-citationSung, Y., Lue, N., Hamza, B. M., Martel, J., Irimia, D., Dasari, R. R., Choi, W., Yaqoob, Z., & So, P. T. C. (2014) · Physical Review Applied · doi:10.1103/physrevapplied.1.014002 · Cited by 111 · see citing works
Aknoun, S., Savatier, J., Bon, P., Galland, F., Abdeladim, L., Wattellier, B. F., & Monneret, S. (2015) · Journal of Biomedical Optics · doi:10.1117/1.jbo.20.12.126009 · Cited by 111 · see citing works
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Weaver, W. M., Tseng, P., Kunze, A., Masaeli, M., Chung, A. J., Dudani, J. S., Kittur, H., Kulkarni, R. P., & Carlo, D. D. (2013) · Current Opinion in Biotechnology · doi:10.1016/j.copbio.2013.09.005 · Cited by 103 · see citing works
Corbin, E. A., Kong, F., Lim, C. T., King, W. P., & Bashir, R. (2014) · Lab on a Chip · doi:10.1039/c4lc01179a · Cited by 91 · see citing works
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Miettinen, T. P., Caldez, M. J., Kaldis, P., & Björklund, M. (2017) · BioEssays · doi:10.1002/bies.201700058 · Cited by 89 · see citing works
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Taşoğlu, S., Khoory, J. A., Tekin, H. C., Thomas, C., Karnoub, A. E., Ghiran, I. C., & Demirci, U. (2015) · Advanced Materials · doi:10.1002/adma.201405660 · Cited by 89 · see citing works
Cell tension and mechanical regulation of cell volume
Gonzalez, N. P., Tao, J., Rochman, N. D., Vig, D., Chiu, E., Wirtz, D., & Sun, S. X. (2018) · Molecular Biology of the Cell · doi:10.1091/mbc.e18-04-0213 · Cited by 87 · see citing works
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Xie, S., Swaffer, M. P., & Skotheim, J. M. (2022) · Annual Review of Cell and Developmental Biology · doi:10.1146/annurev-cellbio-120219-040142 · Cited by 89 · see citing works
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Chen, Y., Zhao, G., Zahumenský, J., Honey, S. A., & Futcher, B. (2020) · Molecular Cell · doi:10.1016/j.molcel.2020.03.012 · Cited by 82 · see citing works
Noninvasive monitoring of single-cell mechanics by acoustic scattering
Kang, J. H., Miettinen, T. P., Chen, L., Olçum, S., Katsikis, G., Doyle, P. S., & Manalis, S. R. (2019) · Nature Methods · doi:10.1038/s41592-019-0326-x · Cited by 82 · see citing works
Self-citationOn the Molecular Mechanisms Regulating Animal Cell Size Homeostasis
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Xie, S., & Skotheim, J. M. (2020) · Current Biology · doi:10.1016/j.cub.2019.12.062 · Cited by 78 · see citing works
Sizing up to Divide: Mitotic Cell-Size Control in Fission Yeast
Wood, E., & Nurse, P. (2015) · Annual Review of Cell and Developmental Biology · doi:10.1146/annurev-cellbio-100814-125601 · Cited by 78 · see citing works
Smart Cell Culture Systems: Integration of Sensors and Actuators into Microphysiological Systems
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Current Trends of Microfluidic Single-Cell Technologies
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Liu, S., Ginzberg, M. B., Patel, N., Hild, M., Leung, B., Li, Z., Chen, Y., Chang, N. W., Wang, Y., Tan, C., et al. (2018) · eLife · doi:10.7554/elife.26947 · Cited by 69 · see citing works
Self-citationStructural biases in disordered proteins are prevalent in the cell
MOSES, D. C., Guadalupe, K. C., Yu, F., Flores, E., Perez, A. R., McAnelly, R. L., Shamoon, N. M., Kaur, G., Cuevas-Zepeda, E., Merg, A. D., et al. (2024) · Nature Structural & Molecular Biology · doi:10.1038/s41594-023-01148-8 · Cited by 66 · see citing works
Systematic identification of cell size regulators in budding yeast
Soifer, I., & Barkai, N. (2014) · Molecular Systems Biology · doi:10.15252/msb.20145345 · Cited by 68 · see citing works
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Self-citationMu, L., Kang, J. H., Olçum, S., Payer, K. R., Calistri, N. L., Kimmerling, R. J., Manalis, S. R., & Miettinen, T. P. (2020) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1922197117 · Cited by 61 · see citing works
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Self-citationMir, M., Bergamaschi, A., Katzenellenbogen, B. S., & Popescu, G. (2014) · PLoS ONE · doi:10.1371/journal.pone.0089000 · Cited by 59 · see citing works
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Perez-Gonzalez, N. A., Rochman, N. D., Yao, K., Tao, J., Le, M.-T. T., Flanary, S. M., Sablich, L., Toler, B., Crentsil, E. U., Takaesu, F., et al. (2019) · The Journal of Cell Biology · doi:10.1083/jcb.201902067 · Cited by 58 · see citing works
Deconstructing stem cell population heterogeneity: Single-cell analysis and modeling approaches
Wu, J., & Tzanakakis, E. S. (2013) · Biotechnology Advances · doi:10.1016/j.biotechadv.2013.09.001 · Cited by 57 · see citing works
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Kim, J., Song, J., Kim, K., Kim, S., Song, J., Kim, N., Khan, M. F., Zhang, L., Sader, J. E., Park, K., et al. (2016) · Nano Letters · doi:10.1021/acs.nanolett.5b03703 · Cited by 57 · see citing works
Self-citationLiu, X., Oh, S., Peshkin, L., & Kirschner, M. W. (2020) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2002152117 · Cited by 55 · see citing works
Self-citationZhao, Y., Lai, H. S. S., Zhang, G., Lee, G., & Li, W. J. (2014) · Lab on a Chip · doi:10.1039/c4lc00795f · Cited by 54 · see citing works
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Osella, M., Tans, S. J., & Lagomarsino, M. C. (2017) · Trends in Microbiology · doi:10.1016/j.tim.2016.12.005 · Cited by 52 · see citing works
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Agache, V., Blanco-Gomez, G., Baleras, F., & Caillat, P. (2011) · Lab on a Chip · doi:10.1039/c1lc20011a · Cited by 49 · see citing works
Kafri, M., Metzl-Raz, E., Jonas, F., & Barkai, N. (2016) · FEMS Yeast Research · doi:10.1093/femsyr/fow081 · Cited by 48 · see citing works
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Miettinen, T. P., Kang, J. H., Yang, L. F., & Manalis, S. R. (2019) · eLife · doi:10.7554/elife.44700 · Cited by 48 · see citing works
Self-citationSarangapani, S., Patil, A., Ngeow, Y. K., Mohan, R. E., Asundi, A., & Lang, M. J. (2018) · Integrative Biology · doi:10.1039/c8ib00038g · Cited by 45 · see citing works
Dario, M. D., & Sablowski, R. W. M. (2019) · Annual Review of Genetics · doi:10.1146/annurev-genet-112618-043602 · Cited by 44 · see citing works
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Jeynes, J. C. G., Jeynes, C., Merchant, M. J., & Kirkby, K. J. (2013) · The Analyst · doi:10.1039/c3an01406a · Cited by 43 · see citing works
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Avesar, J., Arye, T. B., & Levenberg, S. (2014) · Lab on a Chip · doi:10.1039/c4lc00013g · Cited by 42 · see citing works
Lee, J., Bechstein, D. J. B., Ooi, C. C., Patel, A., Gaster, R. S., Ng, E., Gonzalez, L. C., & Wang, S. X. (2016) · Nature Communications · doi:10.1038/ncomms12220 · Cited by 41 · see citing works
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Wang, M., Yang, Y., Han, L., Xu, F., & Li, F. (2019) · Journal of Cellular Physiology · doi:10.1002/jcp.29341 · Cited by 39 · see citing works
Stockslager, M. A., Malinowski, S. W., Touat, M., Yoon, J. C., Geduldig, J. E., Mirza, M., Kim, A. S., Wen, P. Y., Chow, K.-H., Ligon, K. L., et al. (2021) · Cell Reports · doi:10.1016/j.celrep.2021.109788 · Cited by 36 · see citing works
Self-citationKang, J. H., Katsikis, G., Li, Z., Sapp, K. M., Stockslager, M. A., Lim, D., Heiden, M. G. V., Yaffe, M. B., Manalis, S. R., & Miettinen, T. P. (2020) · Nature Communications · doi:10.1038/s41467-020-18769-y · Cited by 37 · see citing works
Self-citationHuang, J., Wang, L., Xiong, C., & Yuan, F. (2016) · Biomaterials · doi:10.1016/j.biomaterials.2016.04.024 · Cited by 37 · see citing works
Cytokinetic abscission is an acute G1 event
Gershony, O., Pe’er, T., Noach-Hirsh, M., Elia, N., & Tzur, A. (2014) · Cell Cycle · doi:10.4161/15384101.2014.956486 · Cited by 36 · see citing works
Self-citationPersistence to anti-cancer treatments in the stationary to proliferating transition
Mizrahi, S. P., Gefen, O., Simon, I., & Balaban, N. Q. (2016) · Cell Cycle · doi:10.1080/15384101.2016.1248006 · Cited by 32 · see citing works
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Chadha, Y., Khurana, A., & Schmoller, K. M. (2024) · Physiological Reviews · doi:10.1152/physrev.00046.2023 · Cited by 31 · see citing works
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Kolitz, S. E., & Lauffenburger, D. A. (2012) · Biochemistry · doi:10.1021/bi300846p · Cited by 33 · see citing works
Controlling Structural Bias in Intrinsically Disordered Proteins Using Solution Space Scanning
Holehouse, A. S., & Sukenik, S. (2020) · Journal of Chemical Theory and Computation · doi:10.1021/acs.jctc.9b00604 · Cited by 33 · see citing works
Constitutive expression of a fluorescent protein reports the size of live human cells
Berenson, D. F., Zatulovskiy, E. A., Xie, S., & Skotheim, J. M. (2019) · Molecular Biology of the Cell · doi:10.1091/mbc.e19-03-0171 · Cited by 42 · see citing works
Nanomechanical identification of liquid reagents in a microfluidic channel
Khan, M. F., Kim, S., Lee, D., Schmid, S., Boisen, A., & Thundat, T. G. (2014) · Lab on a Chip · doi:10.1039/c3lc51273h · Cited by 32 · see citing works
The interface of nanoparticles with proliferating mammalian cells
Panet, E., Mashriki, T., Lahmi, R., Jacob, A., Ozer, E., Vecsler, M., Lazar, I., & Tzur, A. (2017) · Nature Nanotechnology · doi:10.1038/nnano.2017.140 · Cited by 31 · see citing works
Self-citationThe phenomenology of cell size control
Schmoller, K. M. (2017) · Current Opinion in Cell Biology · doi:10.1016/j.ceb.2017.11.011 · Cited by 30 · see citing works
Mechano-Optical Analysis of Single Cells with Transparent Microcapillary Resonators
Martín-Pérez, A., Ramos, D., Gil-Santos, E., García‐López, S., Yubero, M. L., Kosaka, P. M., Paulo, Á. S., Tamayo, J., & Calleja, M. (2019) · ACS Sensors · doi:10.1021/acssensors.9b02038 · Cited by 30 · see citing works
A Review on Theory and Modelling of Nanomechanical Sensors for Biological Applications
Ruz, J. J., Malvar, Ó., Gil-Santos, E., Ramos, D., Calleja, M., & Tamayo, J. (2021) · Processes · doi:10.3390/pr9010164 · Cited by 29 · see citing works
Continuous microcarrier-based cell culture in a benchtop microfluidic bioreactor
Abeille, F., Mittler, F., Obeïd, P., Huet, M., Kermarrec, F., Dolega, M. E., Navarro, F. P., Pouteau, P., Icard, B., Gidrol, X., et al. (2014) · Lab on a Chip · doi:10.1039/c4lc00570h · Cited by 29 · see citing works
Modena, M. M., Wang, Y., Riedel, D., & Burg, T. P. (2013) · Lab on a Chip · doi:10.1039/c3lc51058a · Cited by 27 · see citing works
Transcriptional Networks Controlling the Cell Cycle
Bonke, M., Turunen, M. M., Sokolova, M., Vähärautio, A., Kivioja, T., Taipale, M., Björklund, M., & Taipale, J. (2013) · G3 Genes Genomes Genetics · doi:10.1534/g3.112.004283 · Cited by 25 · see citing works
Dynamics of living cells in a cytomorphological state space
Chang, A. Y., & Marshall, W. F. (2019) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1902849116 · Cited by 24 · see citing works
Opto-acoustic sensing of fluids and bioparticles with optomechanofluidic resonators
Zhu, K., Han, K., Carmon, T., Fan, X., & Bahl, G. (2014) · The European Physical Journal Special Topics · doi:10.1140/epjst/e2014-02237-4 · Cited by 24 · see citing works
Classic “broken cell” techniques and newer live cell methods for cell cycle assessment
Henderson, L. M., Bortone, D. S., Lim, C., & Zambon, A. C. (2013) · American Journal of Physiology-Cell Physiology · doi:10.1152/ajpcell.00006.2013 · Cited by 23 · see citing works
Unbalanced Growth, Senescence and Aging
Polymenis, M., & Kennedy, B. K. (2017) · Advances in experimental medicine and biology · doi:10.1007/978-3-319-57127-0_8 · Cited by 23 · see citing works
High density is a property of slow-cycling and treatment-resistant human glioblastoma cells
Sabelström, H., Quigley, D. A., Fenster, T., Foster, D. J., Fuchshuber, C. A. M., Saxena, S., Yuan, E., Li, N., Paterno, F., Phillips, J. J., et al. (2019) · Experimental Cell Research · doi:10.1016/j.yexcr.2019.03.003 · Cited by 23 · see citing works
Dye exclusion microfluidic microscopy
Schonbrun, E. F., Caprio, G. D., & Schaak, D. (2013) · Optics Express · doi:10.1364/oe.21.008793 · Cited by 22 · see citing works
- Authors
- Son, S., Tzur, A., Weng, Y., Jorgensen, P., Kim, J., Kirschner, M. W., & Manalis, S. R.
- Affiliations
- Koch Institute for Integrative Cancer Research At MIT (US)
- Harvard University (US)
- Massachusetts Institute of Technology (US)
- Corresponding author
- Manalis, Scott R
- Venue
- Nature Methods
- Published
- 2012-08-05
- Volume
- 9
- Issue
- 9
- Pages
- 910-912
- ISSN
- 1548-7091 (ISSN-L)
- 1548-7105
- PMC
- PMC3433595
- OpenAlex
- W1524987177
- Cited by
- 220
- References
- 15
- Citations by year
- 2026: 4 · 2025: 8 · 2024: 11 · 2023: 5 · 2022: 17 · 2021: 12 · 2020: 18 · 2019: 20 · 2018: 18 · 2017: 20 · 2016: 19 · 2015: 22 · 2014: 21 · 2013: 20 · 2012: 4
- FWCI
- 8.62
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Nature Methods (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Digital Access to Scholarship at Harvard (DASH) (Harvard University) (submittedVersion, cc-by)
- DSpace@MIT (Massachusetts Institute of Technology) (submittedVersion, cc-by-nc)
- PMC
- Funders
- National Institutes of Health (5p50gm068762-10)
- National Cancer Institute (R21CA137695, U54CA143874)
- National Institute of General Medical Sciences (R01GM026875, P50-GM068762, R01GM085457)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gene Regulatory Network Analysis
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Cell Image Analysis Techniques
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Advanced Fluorescence Microscopy Techniques
- Keywords
- cell growth, cell size control, cell cycle progression, microfluidics, growth rate variability, G1/S transition, cell lineage analysis, lymphoblastoid cell lines
- MeSH terms
- Animals, Cell Line, Lymphocytes, G1 Phase, S Phase, Cell Lineage, Microfluidic Analytical Techniques, Cell Size, Cell Enlargement, Cell Proliferation, Mice, Precursor Cells, B-Lymphoid, Single-Cell Analysis
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