The DOI matches its Crossref record; 6 of the 6 references it cites were found in a registry or an index.
An optimized fluorescent probe for visualizing glutamate neurotransmission
Open accessAbstract
We describe an intensity-based glutamate-sensing fluorescent reporter (iGluSnFR) with signal-to-noise ratio and kinetics appropriate for in vivo imaging. We engineered iGluSnFR in vitro to maximize its fluorescence change, and we validated its utility for visualizing glutamate release by neurons and astrocytes in increasingly intact neurological systems. In hippocampal culture, iGluSnFR detected single field stimulus–evoked glutamate release events. In pyramidal neurons in acute brain slices, glutamate uncaging at single spines showed that iGluSnFR responds robustly and specifically to glutamate in situ, and responses correlate with voltage changes. In mouse retina, iGluSnFR-expressing neurons showed intact light-evoked excitatory currents, and the sensor revealed tonic glutamate signaling in response to light stimuli. In worms, glutamate signals preceded and predicted postsynaptic calcium transients. In zebrafish, iGluSnFR revealed spatial organization of direction-selective synaptic activity in the optic tectum. Finally, in mouse forelimb motor cortex, iGluSnFR expression in layer V pyramidal neurons revealed task-dependent single-spine activity during running.
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Cites
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Transparent Adult Zebrafish as a Tool for In Vivo Transplantation Analysis
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New roles for astrocytes: redefining the functional architecture of the brain.
Nedergaard, M., Ransom, B., & Goldman, S. A. (2003) · Trends in Neurosciences · doi:10.1016/j.tins.2003.08.008 · Cited by 1,334 · cited 7×
“A uthor M anuscript A uthor M anuscript A uthor M anuscript A uthor M anuscript A uthor M anuscript A uthor M anuscript A uthor M anuscript A uthor M anuscript with line scans (500 lps, ROI contained the cross section of a single, iGluSnFR-expressing dendrite) in the OFF-layer of the IPL during constant background ill…”
BackgroundInfluentialStably maintained dendritic spines are associated with lifelong memories
Yang, G., Pan, F., & Gan, W.-B. (2009) · Nature · doi:10.1038/nature08577 · Cited by 1,182
Self-citationThe neural circuit for touch sensitivity in Caenorhabditis elegans
Chalfie, M., Sulston, J., White, J., Southgate, E., Thomson, J., & Brenner, S. (1985) · The Journal of Neuroscience · doi:10.1523/jneurosci.05-04-00956.1985 · Cited by 1,093
Synaptic amplification by dendritic spines enhances input cooperativity
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Cited by
Madisen, L., Garner, A. R., Shimaoka, D., Chuong, A. S., Klapoetke, N. C., Li, L., van der Bourg, A., Niino, Y., Egolf, L., Monetti, C., et al. (2015) · Neuron · doi:10.1016/j.neuron.2015.02.022 · Cited by 1,114
A gut-brain neural circuit for nutrient sensory transduction
Kaelberer, M. M., Buchanan, K. L., Klein, M. E., Barth, B. B., Montoya, M. M., Shen, X., & Bohórquez, D. V. (2018) · Science · doi:10.1126/science.aat5236 · Cited by 932
Sun, F., Zeng, J., Jing, M., Zhou, J., Feng, J., Owen, S. F., Luo, Y., Li, F., Wang, H., Yamaguchi, T., et al. (2018) · Cell · doi:10.1016/j.cell.2018.06.042 · Cited by 837
Chai, H., Diaz-Castro, B., Shigetomi, E., Monte, E., Octeau, J. C., Yu, X., Cohn, W., Rajendran, P. S., Vondriska, T. M., Whitelegge, J. P., et al. (2017) · Neuron · doi:10.1016/j.neuron.2017.06.029 · Cited by 769
A genetically encoded fluorescent sensor for rapid and specific in vivo detection of norepinephrine
Feng, J., Zhang, C., Lischinsky, J. E., Jing, M., Zhou, J., Wang, H., Zhang, Y., Dong, A., Wu, Z., Wu, H., et al. (2019) · Neuron · doi:10.1016/j.neuron.2019.02.037 · Cited by 545
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Metadata
- Authors
- Marvin, J. S., Borghuis, B. G., Tian, L., Cichon, J., Harnett, M. T., Akerboom, J., Gordus, A., Renninger, S. L., Chen, T.-W., Bargmann, C. I., Orger, M. B., Schreiter, E. R., Demb, J. B., Gan, W.-B., Hires, S. A., & Looger, L. L.
- Venue
- Nature Methods
- Published
- 2013-01-13
- Volume
- 10
- Issue
- 2
- Pages
- 162-170
- ISSN
- 1548-7091 (ISSN-L)
- 1548-7105
- OpenAlex
- W2147800368
- Cited by
- 1,000
- References
- 6
- FWCI
- 27.90
- Type
- journal article
- Language
- en
- Open access
- green
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
