The DOI matches its Crossref record; 4 of the 4 references it cites were found in a registry or an index.
Directed evolution of APEX2 for electron microscopy and proximity labeling
Open accessAbstract
APEX is an engineered peroxidase that functions as an electron microscopy tag and a promiscuous labeling enzyme for live-cell proteomics. Because limited sensitivity precludes applications requiring low APEX expression, we used yeast-display evolution to improve its catalytic efficiency. APEX2 is far more active in cells, enabling the use of electron microscopy to resolve the submitochondrial localization of calcium uptake regulatory protein MICU1. APEX2 also permits superior enrichment of endogenous mitochondrial and endoplasmic reticulum membrane proteins.
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Engineered ascorbate peroxidase as a genetically encoded reporter for electron microscopy
Martell, J. D., Deerinck, T. J., Sancak, Y., Poulos, T. L., Mootha, V. K., Sosinsky, G. E., Ellisman, M. H., & Ting, A. Y. (2012) · Nature Biotechnology · doi:10.1038/nbt.2375 · Cited by 722
Self-citationHung, V., Zou, P., Rhee, H.-W., Udeshi, N. D., Cracan, V., Svinkina, T., Carr, S. A., Mootha, V. K., & Ting, A. Y. (2014) · Molecular Cell · doi:10.1016/j.molcel.2014.06.003 · Cited by 517
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Efficient proximity labeling in living cells and organisms with TurboID
Branon, T. C., Bosch, J. A., Sanchez, A. D., Udeshi, N. D., Svinkina, T., Carr, S. A., Feldman, J. L., Perrimon, N., & Ting, A. Y. (2018) · Nature Biotechnology · doi:10.1038/nbt.4201 · Cited by 2,174
Self-citationThe next generation of CRISPR–Cas technologies and applications
Pickar-Oliver, A., & Gersbach, C. A. (2019) · Nature Reviews Molecular Cell Biology · doi:10.1038/s41580-019-0131-5 · Cited by 1,459
G3BP1 is a tunable switch that triggers phase separation to assemble stress granules
Yang, P., Mathieu, C., Kolaitis, R.-M., Zhang, P., Messing, J., Yurtsever, U., Yang, Z., Wu, J., Li, Y., Pan, Q., et al. (2020) · Cell · doi:10.1016/j.cell.2020.03.046 · Cited by 1,249
Daigle, T. L., Madisen, L., Hage, T. A., Valley, M. T., Knoblich, U., Larsen, R. S., Takeno, M. M., Huang, L., Gu, H., Larsen, R., et al. (2018) · Cell · doi:10.1016/j.cell.2018.06.035 · Cited by 817
Deciphering molecular interactions by proximity labeling
Qin, W., Cho, K. F., Cavanagh, P. E., & Ting, A. Y. (2021) · Nature Methods · doi:10.1038/s41592-020-01010-5 · Cited by 671
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Metadata
- Authors
- Lam, S. S., Martell, J. D., Kamer, K. J., Deerinck, T. J., Ellisman, M. H., Mootha, V. K., & Ting, A. Y.
- Venue
- Nature Methods
- Published
- 2014-11-24
- Volume
- 12
- Issue
- 1
- Pages
- 51-54
- ISSN
- 1548-7091 (ISSN-L)
- 1548-7105
- OpenAlex
- W2023949937
- Cited by
- 1,377
- References
- 4
- FWCI
- 22.78
- Type
- journal article
- Language
- en
- Open access
- bronze
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