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A naturally monomeric infrared fluorescent protein for protein labeling in vivo
Open accessAbstract
Infrared fluorescent proteins (IFPs) provide an additional color to GFP and its homologs in protein labeling. Drawing on structural analysis of the dimer interface, we identified a bacteriophytochrome in the sequence database that is monomeric in truncated form and engineered it into a naturally monomeric IFP (mIFP). We demonstrate that mIFP correctly labels proteins in live cells, Drosophila and zebrafish. It should be useful in molecular, cell and developmental biology.
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Cites
Shaner, N. C., Campbell, R. E., Steinbach, P. A., Giepmans, B. N. G., Palmer, A. E., & Tsien, R. Y. (2004) · Nature Biotechnology · doi:10.1038/nbt1037 · Cited by 4,619
Cited by
Bright monomeric near-infrared fluorescent proteins as tags and biosensors for multiscale imaging
Shcherbakova, D. M., Baloban, M., Emelyanov, A. V., Brenowitz, M., Guo, P., & Verkhusha, V. V. (2016) · Nature Communications · doi:10.1038/ncomms12405 · Cited by 312
Near-Infrared Fluorescent Proteins, Biosensors, and Optogenetic Tools Engineered from Phytochromes
Chernov, K. G., Redchuk, T. A., Omelina, E. S., & Verkhusha, V. V. (2017) · Chemical Reviews · doi:10.1021/acs.chemrev.6b00700 · Cited by 264
Chen, M., Feng, Z., Fan, X., Sun, J., Geng, W., Wu, T., Sheng, J., Qian, J., & Xu, Z. (2022) · Nature Communications · doi:10.1038/s41467-022-34274-w · Cited by 57
Metadata
- Authors
- Yu, D., Baird, M. A., Allen, J. R., Howe, E. S., Klassen, M. P., Reade, A., Makhijani, K., Song, Y., Liu, S., Murthy, Z., Zhang, S.-Q., Weiner, O. D., Kornberg, T. B., Jan, Y.-N., Davidson, M. W., & Shu, X.
- Venue
- Nature Methods
- Published
- 2015-06-22
- Volume
- 12
- Issue
- 8
- Pages
- 763-765
- ISSN
- 1548-7091 (ISSN-L)
- 1548-7105
- OpenAlex
- W2194418093
- Cited by
- 168
- References
- 1
- FWCI
- 9.20
- Type
- journal article
- Language
- en
- Open access
- green
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- Yu, D., Baird, M. A., Allen, J. R., Howe, E. S., Klassen, M. P., Reade, A., Makhijani, K., Song, Y., Liu, S., Murthy, Z., Zhang, S.-Q., Weiner, O. D., Kornberg, T. B., Jan, Y.-N., Davidson, M. W., & Shu, X. (2015). A naturally monomeric infrared fluorescent protein for protein labeling in vivo. Nature Methods. https://doi.org/10.1038/nmeth.3447
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