Long-term monitoring of intravital biological processes using fluorescent protein-assisted NIR-II imaging
Open accessAbstract
High spatial resolution, low background, and deep tissue penetration have made near-infrared II (NIR-II) fluorescence imaging one of the most critical tools for in vivo observation and measurement. However, the relatively short retention time and potential toxicity of synthetic NIR-II fluorophores limit their long-term application. Here, we report the use of infrared fluorescent proteins (iRFPs) as in vitro and in vivo NIR-II probes permitting prolonged continuous imaging (up to 15 months). As a representative example, iRFP713 is knocked into the mouse genome to generate a transgenic model to allow temporal and/or spatial expression control of the probe. To demonstrate its feasibility in a genuine diagnostic context, we adopt two liver regeneration models and successfully track the process for a week. The performance and monitoring efficacy are comparable to those of μCT and superior to those of indocyanine green dye. We are also able to effectively observe the pancreas, despite its deep location, under both physiological and pathological conditions. These results indicate that the iRFP-assisted NIR-II fluorescence system is suitable for monitoring various tissues and in vivo biological processes, providing a powerful noninvasive long-term imaging platform.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
NIR-II fluorophores have deep-tissue penetration, reduced tissue autofluorescence, and low phototoxicity.
“Near-infrared II (NIR-II) fluorophores possess transformative potential for biomedical applications, owing to their deep-tissue penetration, reduced tissue autofluorescence, and low phototoxicity.”
From Organic small-molecule NIR-II fluorophores for tumor phototheranostics (2026) · cites this paper as [40]
The reduced absorption and scattering of NIR-II light suppresses background noise, minimizes tissue autofluorescence, and results in negligible phototoxicity.
“This fundamental property suppresses background noise, minimizes tissue autofluorescence, and results in negligible phototoxicity39,40.”
From Organic small-molecule NIR-II fluorophores for tumor phototheranostics (2026) · cites this paper as [40]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
Liver regeneration: from myth to mechanism
Taub, R. (2004) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm1489 · Cited by 1,567 · see citing works
Hu, Z., Fang, C., Li, B., Zhang, Z., Cao, C., Cai, M., Su, S., Sun, X., Shi, X., Li, C., et al. (2019) · Nature Biomedical Engineering · doi:10.1038/s41551-019-0494-0 · Cited by 1,126 · see citing works
Efficient in vivo manipulation of mouse genomic sequences at the zygote stage.
Lakso, M., Pichel, J. G., Gorman, J. R., Sauer, B., Okamoto, Y., Lee, E., Alt, F. W., & Westphal, H. (1996) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.93.12.5860 · Cited by 1,125 · see citing works
Boxhoorn, L., Voermans, R. P., Bouwense, S. A. W., Bruno, M. J., Verdonk, R. C., Boermeester, M. A., van Santvoort, H. C., & Besselink, M. G. (2020) · The Lancet · doi:10.1016/s0140-6736(20)31310-6 · Cited by 1,111 · see citing works
Advanced Fluorescence Imaging Technology in the Near-Infrared-II Window for Biomedical Applications
Li, C., Chen, G., Zhang, Y., Wu, F., & Wang, Q. (2020) · Journal of the American Chemical Society · doi:10.1021/jacs.0c07022 · Cited by 1,037 · see citing works
He, S., Song, J., Qu, J., & Cheng, Z. (2018) · Chemical Society Reviews · doi:10.1039/c8cs00234g · Cited by 1,021 · see citing works
Multifunctional in vivo vascular imaging using near-infrared II fluorescence
Hong, G., Lee, J. C., Robinson, J. T., Raaz, U., Xie, L., Huang, N. F., Cooke, J. P., & Dai, H. (2012) · Nature Medicine · doi:10.1038/nm.2995 · Cited by 995 · see citing works
Diabetes Mellitus and the β Cell: The Last Ten Years
Ashcroft, F. M., & Rorsman, P. (2012) · Cell · doi:10.1016/j.cell.2012.02.010 · Cited by 902 · see citing works
Carr, J. A., Franke, D., Caram, J. R., Perkinson, C. F., Saif, M., Askoxylakis, V., Datta, M., Fukumura, D., Jain, R. K., Bawendi, M. G., et al. (2018) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1718917115 · Cited by 721 · see citing works
X-ray-activated persistent luminescence nanomaterials for NIR-II imaging
Pei, P., Chen, Y., Sun, C., Fan, Y., Yang, Y., Liu, X., Lu, L., Zhao, M., Zhang, H., Zhao, D., et al. (2021) · Nature Nanotechnology · doi:10.1038/s41565-021-00922-3 · Cited by 718 · see citing works
Next-generation in vivo optical imaging with short-wave infrared quantum dots
Bruns, O. T., Bischof, T. S., Harris, D. K., Franke, D., Shi, Y., Riedemann, L., Bartelt, A., Jaworski, F. B., Carr, J. A., Rowlands, C. J., et al. (2017) · Nature Biomedical Engineering · doi:10.1038/s41551-017-0056 · Cited by 686 · see citing works
Bright and stable near-infrared fluorescent protein for in vivo imaging
Filonov, G. S., Piatkevich, K. D., Ting, L.-M., Zhang, J., Kim, K., & Verkhusha, V. V. (2011) · Nature Biotechnology · doi:10.1038/nbt.1918 · Cited by 682 · see citing works
A reproducible and well-tolerated method for 2/3 partial hepatectomy in mice
Mitchell, C., & Willenbring, H. (2008) · Nature Protocols · doi:10.1038/nprot.2008.80 · Cited by 577 · see citing works
Zhong, Y., Ma, Z., Wang, F., Wang, X., Yang, Y., Liu, Y., Zhao, X., Li, J., Du, H., Zhang, M., et al. (2019) · Nature Biotechnology · doi:10.1038/s41587-019-0262-4 · Cited by 575 · see citing works
Clinical applications of indocyanine green (ICG) enhanced fluorescence in laparoscopic surgery
Boni, L., David, G., Mangano, A., Dionigi, G., Rausei, S., Spampatti, S., Cassinotti, E., & Fingerhut, A. L. (2014) · Surgical Endoscopy · doi:10.1007/s00464-014-3895-x · Cited by 550 · see citing works
Near-infrared fluorescent proteins for multicolor in vivo imaging
Shcherbakova, D. M., & Verkhusha, V. V. (2013) · Nature Methods · doi:10.1038/nmeth.2521 · Cited by 541 · see citing works
Bright quantum dots emitting at ∼1,600 nm in the NIR-IIb window for deep tissue fluorescence imaging
Zhang, M., Yue, J., Cui, R., Ma, Z., Wan, H., Wang, F., Zhu, S., Zhou, Y., Kuang, Y., Zhong, Y., et al. (2018) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1806153115 · Cited by 432 · see citing works
Perfecting and extending the near-infrared imaging window
Feng, Z., Tang, T., Wu, T., Yu, X., Zhang, Y., Wang, M., Zheng, J., Ying, Y., Chen, S., Zhou, J., et al. (2021) · Light: Science & Applications · doi:10.1038/s41377-021-00628-0 · Cited by 394 · see citing works
Self-citationYang, W., Tao, Y., Wu, Y., Zhao, X., Ye, W., Zhao, D., Fu, L., Tian, C., Yang, J., He, F., et al. (2019) · Nature Communications · doi:10.1038/s41467-019-09046-8 · Cited by 367 · see citing works
Epithelial-Specific Cre/lox Recombination in the Developing Kidney and Genitourinary Tract
Shao, X., Somlo, S., & Igarashi, P. (2002) · Journal of the American Society of Nephrology · doi:10.1097/01.asn.0000016444.90348.50 · Cited by 332 · see citing works
Recent Progress in NIR-II Contrast Agent for Biological Imaging
Cao, J., Zhu, B., Zheng, K., He, S., Liang, M., Song, J., & Yang, H. (2020) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2019.00487 · Cited by 314 · see citing works
A hybrid erbium(III)–bacteriochlorin near-infrared probe for multiplexed biomedical imaging
Wang, T., Wang, S., Liu, Z., He, Z., Yu, P., Zhao, M., Zhang, H., Lu, L., Wang, Z., Wang, Z., et al. (2021) · Nature Materials · doi:10.1038/s41563-021-01063-7 · Cited by 308 · see citing works
Use of Fluorescent Probes: Their Effect on Cell Biology and Limitations
Jensen, E. C. (2012) · The Anatomical Record · doi:10.1002/ar.22602 · Cited by 253 · see citing works
Colloidal Alloyed Quantum Dots with Enhanced Photoluminescence Quantum Yield in the NIR-II Window
Yang, H., Li, R., Zhang, Y., Yu, M., Wang, Z., Liu, X., You, W., Tu, D., Sun, Z., Zhang, R., et al. (2021) · Journal of the American Chemical Society · doi:10.1021/jacs.0c13071 · Cited by 241 · see citing works
Feng, Z., Yu, X., Jiang, M., Zhu, L. J., Zhang, Y., Yang, W., Wang, X., Li, G., & Qian, J. (2019) · Theranostics · doi:10.7150/thno.31332 · Cited by 210 · see citing works
Self-citationA phosphorescent probe for in vivo imaging in the second near-infrared window
Chang, B., Li, D., Ren, Y., Qu, C., Shi, X., Liu, R., Liu, H., Tian, J., Hu, Z., Sun, T., et al. (2021) · Nature Biomedical Engineering · doi:10.1038/s41551-021-00773-2 · Cited by 195 · see citing works
High Affinity to Skeleton Rare Earth Doped Nanoparticles for Near-Infrared II Imaging
He, S., Chen, S., Li, D., Wu, Y., Zhang, X., Liu, J., Song, J., Liu, L., Qu, J., & Cheng, Z. (2019) · Nano Letters · doi:10.1021/acs.nanolett.9b00140 · Cited by 180 · see citing works
Liver Regeneration after Acetaminophen Hepatotoxicity
Bhushan, B., & Apte, U. (2019) · The American Journal of Pathology · doi:10.1016/j.ajpath.2018.12.006 · Cited by 179 · see citing works
A naturally monomeric infrared fluorescent protein for protein labeling in vivo
Yu, D., Baird, M. A., Allen, J. R., Howe, E. S., Klassen, M. P., Reade, A., Makhijani, K., Song, Y., Liu, S., Murthy, Z., et al. (2015) · Nature Methods · doi:10.1038/nmeth.3447 · Cited by 169 · see citing works
Wu, L., Ishigaki, Y., Zeng, W., Harimoto, T., Yin, B., Chen, Y., Liao, S., Liu, Y., Sun, Y., Zhang, X., et al. (2021) · Nature Communications · doi:10.1038/s41467-021-26380-y · Cited by 136 · see citing works
Yang, H., Huang, H., Ma, X., Zhang, Y., Yang, X., Yu, M., Sun, Z., Li, C., Wu, F., & Wang, Q. (2021) · Advanced Materials · doi:10.1002/adma.202103953 · Cited by 134 · see citing works
Near-infrared fluorescent proteins engineered from bacterial phytochromes
Shcherbakova, D. M., Baloban, M., & Verkhusha, V. V. (2015) · Current Opinion in Chemical Biology · doi:10.1016/j.cbpa.2015.06.005 · Cited by 130 · see citing works
He, H., Lin, Y., Tian, Z., Zhu, D., Zhang, Z., & Pang, D. (2018) · Small · doi:10.1002/smll.201703296 · Cited by 108 · see citing works
Feng, Z., Bai, S., Qi, J., Sun, C., Zhang, Y., Yu, X., Ni, H., Wu, D., Fan, X., Xue, D., et al. (2021) · Advanced Materials · doi:10.1002/adma.202008123 · Cited by 105 · see citing works
Self-citationNIR‐II Fluorescence Endoscopy for Targeted Imaging of Colorectal Cancer
Suo, Y., Wu, F., Xu, P., Shi, H., Wang, T., Liu, H., & Cheng, Z. (2019) · Advanced Healthcare Materials · doi:10.1002/adhm.201900974 · Cited by 104 · see citing works
Fan, X., Li, Y., Feng, Z., Chen, G., Zhou, J., He, M., Wu, L., Li, S., Qian, J., & Lin, H. M. (2021) · Advanced Science · doi:10.1002/advs.202003972 · Cited by 87 · see citing works
Self-citationCao, C., Jin, Z., Shi, X., Zhang, Z., Xiao, A., Yang, J., Ji, N., Tian, J., & Hu, Z. (2022) · IEEE Transactions on Biomedical Engineering · doi:10.1109/tbme.2022.3143859 · Cited by 77 · see citing works
Streptozotocin is equally diabetogenic whether administered to fed or fasted mice
Chaudhry, Z. Z., Morris, D. L., Moss, D. R., Sims, E. K., Chiong, Y., Kono, T., & Evans‐Molina, C. (2013) · Laboratory Animals · doi:10.1177/0023677213489548 · Cited by 71 · see citing works
Near-Infrared Fluorescent Proteins Engineered from Bacterial Phytochromes in Neuroimaging
Piatkevich, K. D., Suk, H., Kodandaramaiah, S. B., Yoshida, F., DeGennaro, E. M., Drobizhev, M. A., Hughes, T. E., Desimone, R., Boyden, E. S., & Verkhusha, V. V. (2017) · Biophysical Journal · doi:10.1016/j.bpj.2017.09.007 · Cited by 55 · see citing works
In Vivo image Analysis Using iRFP Transgenic Mice
Tran, M. T., Tanaka, J., Hamada, M., Sugiyama, Y., Sakaguchi, S., Nakamura, M., Takahashi, S., & Miwa, Y. (2014) · EXPERIMENTAL ANIMALS · doi:10.1538/expanim.63.311 · Cited by 50 · see citing works
Near-Infrared Fluorescent Proteins and Their Applications
Karasev, M. M., Stepanenko, O. V., Rumyantsev, K. A., Константинович, Т. К., & Verkhusha, V. V. (2019) · Biochemistry (Moscow) · doi:10.1134/s0006297919140037 · Cited by 48 · see citing works
Zhao, Z., Hou, N., Sun, Y., Teng, Y., & Yang, X. Y. (2010) · Journal of genetics and genomics/Journal of Genetics and Genomics · doi:10.1016/s1673-8527(09)60083-7 · Cited by 22 · see citing works
Cardarelli, F. (2020) · International Journal of Molecular Sciences · doi:10.3390/ijms21114164 · Cited by 21 · see citing works
Rogers, O. C., Johnson, D. M., & Firnberg, E. (2019) · Scientific Reports · doi:10.1038/s41598-019-52123-7 · Cited by 17 · see citing works
CDy6, a Photostable Probe for Long-Term Real-Time Visualization of Mitosis and Proliferating Cells
Jeong, Y., Zhai, D., Hennig, H., Samanta, A., Agrawalla, B. K., Bray, M., Carpenter, A. E., & Chang, Y. (2015) · Chemistry & Biology · doi:10.1016/j.chembiol.2014.11.018 · Cited by 14 · see citing works
Will, O., Damm, T., Campbell, G. M., Schönfels, W. V., Açil, Y., Will, M., Chalaris, A., Ayna, M., Drucker, C., & Glüer, C. (2016) · Laboratory Animals · doi:10.1177/0023677216678824 · Cited by 9 · see citing works
Tamoxifen-inducible cardiac-specific Cre transgenic mouse using VIPR2 intron
Chin, H. J., Lee, S., & Lee, D. (2020) · Laboratory Animal Research · doi:10.1186/s42826-020-00065-x · Cited by 3 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
In vivo NIR-II fluorescence imaging for biology and medicine
Wang, F., Zhong, Y., Bruns, O., Liang, Y., & Dai, H. (2024) · Nature Photonics · doi:10.1038/s41566-024-01391-5 · Cited by 564 · see citing works
Aggregation-Induced Emission (AIE), Life and Health
Wang, H., Li, Q., Alam, P., Bai, H., Bhalla, V., Bryce, M. R., Cao, M., Chen, C., Chen, S., Chen, X., et al. (2023) · ACS Nano · doi:10.1021/acsnano.3c03925 · Cited by 401 · see citing works
Self-citationNear-infrared II fluorescence imaging
Schmidt, E. L., Ou, Z., Ximendes, E. C., Cui, H., Keck, C. H. C., Jaque, D., & Hong, G. (2024) · Nature Reviews Methods Primers · doi:10.1038/s43586-024-00301-x · Cited by 351 · see citing works
Chang, B., Chen, J., Bao, J., Sun, T., & Cheng, Z. (2023) · Chemical Reviews · doi:10.1021/acs.chemrev.3c00401 · Cited by 154 · see citing works
Feng, Z., Li, Y., Chen, S., Li, J., Wu, T., Ying, Y., Zheng, J., Zhang, Y., Zhang, J., Fan, X., et al. (2023) · Nature Communications · doi:10.1038/s41467-023-40728-6 · Cited by 146 · see citing works
Self-citationXu, J., Zhu, N., Du, Y., Han, T., Zheng, X., Li, J., & Zhu, S. (2024) · Nature Communications · doi:10.1038/s41467-024-47063-4 · Cited by 75 · see citing works
Yue, R., Li, Z., Liu, H., Wang, Y., Li, Y., Yin, R., Yin, B., Qian, H., Kang, H., Zhang, X., et al. (2024) · Nature Communications · doi:10.1038/s41467-024-50688-0 · Cited by 57 · see citing works
Tang, Y., Xiang, D., & Li, Q. (2025) · Advanced Materials · doi:10.1002/adma.202501184 · Cited by 48 · see citing works
Orchestrated Strategies for Developing Fluorophores for NIR‐II Imaging
Feng, X., Long, W., Liu, Y., Chen, X. S., & Tian, R. (2023) · Advanced Healthcare Materials · doi:10.1002/adhm.202300537 · Cited by 49 · see citing works
Development of Polymethine Dyes for NIR‐II Fluorescence Imaging and Therapy
Chen, X., Li, J., Roy, S., Ullah, Z., Gu, J., Huang, H., Yu, C., Wang, X., Wang, H., Zhang, Y., et al. (2024) · Advanced Healthcare Materials · doi:10.1002/adhm.202304506 · Cited by 48 · see citing works
Zhou, D., Zhang, G., Li, J., Zhuang, Z., Shen, P., Fu, X., Wang, L., Qian, J., Qin, A., & Tang, B. Z. (2024) · ACS Nano · doi:10.1021/acsnano.4c06965 · Cited by 43 · see citing works
Self-citationThe interaction between particles and vascular endothelium in blood flow
Li, X., Zou, J., He, Z., Sun, Y., Song, X., & He, W. (2024) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2024.115216 · Cited by 42 · see citing works
Wang, Y., Zhang, J., Wang, Y., Yu, J., Gao, Y., Yang, Y., Li, X., Wang, H., & Li, S. (2024) · Advanced Functional Materials · doi:10.1002/adfm.202406483 · Cited by 37 · see citing works
Hu, B., Liu, Q., Jiang, Y., Huang, Y., Ji, H., Zhang, J., Wang, X., Shen, X., & Chen, H. (2024) · Angewandte Chemie International Edition · doi:10.1002/anie.202418378 · Cited by 31 · see citing works
Qin, Y., Niu, N., Li, X., Yan, X., Lu, S., Li, Z., Gui, Y., Zhu, J., Xu, L. S., Li, X., et al. (2024) · Aggregate · doi:10.1002/agt2.708 · Cited by 27 · see citing works
Chen, Z., Huang, L., Gao, D., Bao, Z., Hu, D., Zheng, W., Chen, J., Liao, J., Zheng, H., & Sheng, Z. (2024) · Advanced Science · doi:10.1002/advs.202411272 · Cited by 25 · see citing works
Recent advances of photoresponsive nanomaterials for diagnosis and treatment of acute kidney injury
Yao, S. J., Wang, Y., Mou, X., Yang, X., & Cai, Y. (2024) · Journal of Nanobiotechnology · doi:10.1186/s12951-024-02906-6 · Cited by 19 · see citing works
Semiconducting polymer dots for multifunctional integrated nanomedicine carriers
Zhang, Z., Yu, C., Wu, Y., Wang, Z., Xu, H., Yan, Y., Zhan, Z., & Yin, S. (2024) · Materials Today Bio · doi:10.1016/j.mtbio.2024.101028 · Cited by 18 · see citing works
Lanthanide-doped nanoprobes for microRNA detection
Liu, Y., Huang, Y., Luo, Y., Luo, Y., Jiang, L., Gallego‐Ortega, D., Cheng, Y. Y., Gale, P. A., & Bao, G. (2025) · Coordination Chemistry Reviews · doi:10.1016/j.ccr.2025.216644 · Cited by 17 · see citing works
Organic small-molecule NIR-II fluorophores for tumor phototheranostics
Xiang, D., Wang, Z., Zheng, H., Tang, Y., & Li, Q. (2026) · Light: Science & Applications · doi:10.1038/s41377-026-02212-w · Cited by 22 · see citing works
“This fundamental property suppresses background noise, minimizes tissue auto-fl uorescence, and results in negligible phototoxicity 39,40 .”
Advances of Fluorescence Molecular Imaging: NIR‐II Window, Probes, and Tomography
Song, F., Zhang, P., Wu, H., Ma, C., Liu, Z., Cai, R., Feng, Y., He, Y., Dong, X., Tian, Y., et al. (2025) · Laser & Photonics Review · doi:10.1002/lpor.202400275 · Cited by 16 · see citing works
Liu, T., Xia, X., Wang, R., Rong, X., Su, Z., Du, J., Fan, J., Peng, X., & Sun, W. C. (2023) · Advanced Functional Materials · doi:10.1002/adfm.202304347 · Cited by 15 · see citing works
Enhancing Total Optical Throughput of Microscopy with Deep Learning for Intravital Observation
Chen, R., Peng, S., Zhu, L., Meng, J., Fan, X., Feng, Z., Zhang, H., & Qian, J. (2023) · Small Methods · doi:10.1002/smtd.202300172 · Cited by 14 · see citing works
Self-citationAdvanced second near-infrared fluorophores for bioapplications
Bai, Q., Yang, Q., Liu, Y., Li, A., Yin, X.-B., & Wu, S. (2025) · Coordination Chemistry Reviews · doi:10.1016/j.ccr.2025.217146 · Cited by 14 · see citing works
Du, J., Jia, T., Li, D., Ågren, H., He, L., & Chen, G. (2026) · Journal of the American Chemical Society · doi:10.1021/jacs.5c21676 · Cited by 12 · see citing works
Kan, L., Zhang, Y., Luo, Y., Wei, Y., Zhong, J., Gao, Y., Liu, Y., Wang, K., & Li, S. (2024) · ACS Nano · doi:10.1021/acsnano.4c07810 · Cited by 11 · see citing works
Hao, L., Chen, J., Li, Q., Zhang, D., Huang, Q., Zhang, H., & Li, S. (2025) · Interdisciplinary medicine · doi:10.1002/inmd.20240078 · Cited by 13 · see citing works
Atomically Precise Metal Clusters for NIR-II Imaging
Ma, H., Ma, D., Liu, P. X., Wang, H., & Zhang, X. (2026) · Accounts of Chemical Research · doi:10.1021/acs.accounts.5c00837 · Cited by 10 · see citing works
Zou, F., Su, B., Leng, H., Xin, N., Jiang, S., Wei, D., Yang, M. J., Wang, Y., & Fan, H. (2024) · Chinese Chemical Letters · doi:10.1016/j.cclet.2024.109523 · Cited by 8 · see citing works
Hu, B., Liu, Q., Jiang, Y., Huang, Y., Ji, H., Zhang, J., Wang, X., Shen, X., & Chen, H. (2024) · Angewandte Chemie · doi:10.1002/ange.202418378 · Cited by 8 · see citing works
Chang, Z., Cai, J.-J., Liu, C., Zhao, S., Zhang, X., Wang, F., & Sun, Q. (2024) · Advanced Functional Materials · doi:10.1002/adfm.202416366 · Cited by 11 · see citing works
Huang, Y., Wang, L., Huang, Q., Hu, B., Liu, Q., Wang, J., Shen, X., & Chen, H. (2025) · Chinese Journal of Chemistry · doi:10.1002/cjoc.70160 · Cited by 7 · see citing works
Small‐Molecule Fluorescent Probes for Butyrylcholinesterase
Lin, Z., Liao, Y., Tian, D., Liao, J., Chen, Q., & Yin, J. (2024) · ChemMedChem · doi:10.1002/cmdc.202400875 · Cited by 7 · see citing works
Wang, J., Huang, Z., Wu, Y., Jiang, X., Ji, Y., Braeckmans, K., Wang, M., Wang, L., Chen, W. R., Xia, Y., et al. (2025) · BMC Biology · doi:10.1186/s12915-025-02215-w · Cited by 6 · see citing works
Qian, M., Du, J., Jiang, Q., Tao, W., Zhang, J., & Gong, L. (2025) · Journal of Rare Earths · doi:10.1016/j.jre.2025.09.006 · Cited by 5 · see citing works
Versatile Nanolights From Silicon, Carbon and Oxygen Hybrid System for Optical Applications
Song, B., Cui, M., Ji, Y., He, Y., Kang, Z., & Lee, S. (2024) · Advanced Functional Materials · doi:10.1002/adfm.202411981 · Cited by 5 · see citing works
Zhao, Q., Tang, S., Zhong, J., Kan, L., Wei, Y., Yang, Y., Qian, X., Li, N., Wang, Y., Wang, K., et al. (2025) · Advanced Materials · doi:10.1002/adma.202417085 · Cited by 5 · see citing works
Personalized NIR-II probes for comprehensive monitoring and evaluation of dialysis vascular access
Cheng, Y., Ma, S., Gong, W., Sun, B., Du, Y., Dang, Z., Wang, X., Zhu, S., & Xu, Z. (2025) · Biomaterials · doi:10.1016/j.biomaterials.2025.123508 · Cited by 4 · see citing works
Self-citationNanoprobe-Based Near-Infrared II Optical Imaging for Guiding Precision Glioma Therapy
Liang, D. S., Li, W., Li, L., Xue, X., Liu, X., & Hu, C. (2025) · International Journal of Nanomedicine · doi:10.2147/ijn.s523676 · Cited by 4 · see citing works
Tao, S., Liu, X. L., Yu, Y., Xu, K., Peng, J., Shaoliang, Y. A. N., He, L., Shen, Z., Guo, G., & Ru, J. (2026) · Journal of Materials Chemistry B · doi:10.1039/d5tb02269j · Cited by 3 · see citing works
Advances in near-infrared fluorescence imaging for biomedicine
Wu, H., Li, X., Liu, Z., Song, F., Cai, R., Chen, H., & Zhang, G. (2026) · Coordination Chemistry Reviews · doi:10.1016/j.ccr.2026.217892 · Cited by 5 · see citing works
Dang, Z., Ma, S., Sun, B., Du, Y., Wang, Z., Jiang, Z., Xiao, J., Qi, Y., Zhang, H., & Zhu, S. (2025) · SmartMat · doi:10.1002/smm2.70041 · Cited by 2 · see citing works
Sun, M., Chen, M., & Wang, J. (2023) · Current Medicinal Chemistry · doi:10.2174/0929867330666230210093411 · Cited by 2 · see citing works
Self-citationLi, Y.-L., Wen, S., Liu, J., Li, S.-W., Shi, J., & Wang, H. (2025) · Journal of Cellular and Molecular Medicine · doi:10.1111/jcmm.70549 · Cited by 2 · see citing works
Kulhanek, D. L., Wei, Q., Head, J. C., Hellinger, J., Jansen, Z., Gilmour, A. R., Segall-Shapiro, T. H., Brodbelt, J. S., Usama, S. M., & Thyer, R. T. (2026) · Protein Science · doi:10.1002/pro.70610 · Cited by 1 · see citing works
Visualized diagnosis in the second near-infrared window
Yan, H., Ma, D., Ma, H., Zhang, J. H., Zhang, X.-D., & Wang, H. (2026) · Coordination Chemistry Reviews · doi:10.1016/j.ccr.2026.217788 · Cited by 1 · see citing works
Jiang, R., Yang, Z., Li, J., Tan, J., Lei, Q., Zhong, B., Mei, Y., Zhang, Q., Jiang, P., & Zhao, Z. (2026) · Sensors and Actuators B Chemical · doi:10.1016/j.snb.2026.139885 · Cited by 1 · see citing works
Macroscopic label-free biomedical imaging with shortwave infrared Raman scatteringPreprint
Arús, B. A., Yiu, J., Lingg, J. G. P., Hofmann, A., Fumo, A. R., Ji, H., Jethwa, C., Park, R. K., Henderson, J., Mishra, K., et al. (2024) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2024.06.10.597863 · Cited by 1 · see citing works
Non-invasive continuous imaging approaches to evaluate tissue structure and development
Huang, Y. (2023) · Theoretical and Natural Science · doi:10.54254/2753-8818/24/20231149
Chenglei, 叶. Y., Zhiping, 何. H., Xuehan, 王. W., & Aijun, 靳. J. (2025) · Infrared and Laser Engineering · doi:10.3788/irla20250136
In-situ Observation of Fast Chloroplast Dynamics in Intact LeavesPreprint
Geng, W., He, M., & Qian, J. (2025) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2025.10.17.683007
Self-citationJiang, Q., Ying, X., Lu, X., Wei, C., Fu, K., Shaoliang, Y. A. N., Shen, Z., Ru, J., & Guo, G. (2026) · Journal of Materials Chemistry B · doi:10.1039/d6tb00155f
Vis–NIR spectroscopy and deep learning for non-invasive in vivo detection of acute liver injury
Wang, X., Wang, Y., Ma, Q., Jin, A., Li, J., Lin, Y., Zhang, H., & He, Z. (2026) · Spectrochimica Acta Part A Molecular and Biomolecular Spectroscopy · doi:10.1016/j.saa.2026.127888
Geng, W., He, M., & Qian, J. (2026) · Photonics Research · doi:10.1364/prj.596367
Self-citationDo Not Break the Vessels: Structure-Preserving Mean Flow for Vascular Image Translation
Sun, C., Hu, Z., Wang, K., Wu, B., Gao, S., Zheng, R., Xue, C., Zhang, Y., & Zhou, G. (2026) · Lecture notes in computer science · doi:10.1007/978-3-032-38189-7_15
Xu, F.-Y., Lee, W., Shi, K.-C., Saha, U., Cui, Y.-X., James, S., Medina, N. Y. G., Nelson, E. R., & Smith, A. M. (2026) · Bioconjugate chemistry · doi:10.1021/acs.bioconjchem.6c00251
Xu, K., Ran, J., Wang, L., Wang, W., Zhang, J., Yin, X., Zhang, Y., Hu, M., Shao, J., Dong, X., et al. (2026) · Chemical Engineering Journal · doi:10.1016/j.cej.2026.183090
Gao, F., Wu, T., Li, X., Liu, L., Xia, N., & Hu, S. (2026) · Microchemical Journal · doi:10.1016/j.microc.2026.120036
Self-citation
- Authors
- Chen, M., Feng, Z., Fan, X., Sun, J., Geng, W., Wu, T., Sheng, J., Qian, J., & Xu, Z.
- Affiliations
- Sir Run Run Shaw Hospital (CN)
- State Key Laboratory of Modern Optical Instruments (CN)
- Corresponding author
- Sheng, Jinghao
- Qian, Jun
- Xu, Zhengping
- Venue
- Nature Communications
- Published
- 2022-11-04
- Volume
- 13
- Issue
- 1
- Pages
- 6643-6643
- ISSN
- 2041-1723 (ISSN-L)
- PMC
- PMC9636246
- OpenAlex
- W4308195469
- Cited by
- 57
- References
- 47
- Citations by year
- 2026: 14 · 2025: 15 · 2024: 20 · 2023: 8
- FWCI
- 6.12
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- Nature Communications (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- RePEc: Research Papers in Economics (submittedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- National Natural Science Foundation of China (2020-KYY-511108-0007, 82 001 874, 81972612, 61 975 172)
- China Postdoctoral Science Foundation (BX20220260)
- Zhejiang University (61975172, 2020-KYY-511108-0007)
- Fundamental Research Funds for the Central Universities (2020-KYY-511108-0007, 61975172)
- Topics
- Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Optical Imaging and Spectroscopy Techniques
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Advanced Fluorescence Microscopy Techniques
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Spectroscopy Techniques in Biomedical and Chemical Research
- Keywords
- NIR-II fluorescence imaging, transgenic mouse model, liver regeneration, pancreatic imaging, deep tissue imaging
- MeSH terms
- Animals, Biological Phenomena, Fluorescent Dyes, Indocyanine Green, Mice, Optical Imaging
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
