A proposal for validation of antibodies

CRISPR-Cas systems for editing, regulating and targeting genomes

Sander, J. D., & Joung, J. K. (2014) · Nature Biotechnology · doi:10.1038/nbt.2842 · Cited by 3,153 · see citing works

Homogeneous antibody-based proximity extension assays provide sensitive and specific detection of low-abundant proteins in human blood

Lundberg, M., Eriksson, A., Tran, B., Assarsson, E., & Fredriksson, S. (2011) · Nucleic Acids Research · doi:10.1093/nar/gkr424 · Cited by 744 · see citing works

Reproducibility: Standardize antibodies used in research

Bradbury, A., & Plückthun, A. (2015) · Nature · doi:10.1038/518027a · Cited by 647 · see citing works

Antibody validation

Bordeaux, J., Welsh, A. W., Agarwal, S., Killiam, E. J., Baquero, M. T., Hanna, J. A., Anagnostou, V., & Rimm, D. L. (2010) · BioTechniques · doi:10.2144/000113382 · Cited by 597 · see citing works

Targeted Peptide Measurements in Biology and Medicine: Best Practices for Mass Spectrometry-based Assay Development Using a Fit-for-Purpose Approach

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Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells

Stadler, C., Rexhepaj, E., Singan, V. R., Murphy, R. F., Pepperkok, R., Uhlén, M., Simpson, J. C., & Lundberg, E. K. (2013) · Nature Methods · doi:10.1038/nmeth.2377 · Cited by 263 · see citing works

Large-Scale Interlaboratory Study to Develop, Analytically Validate and Apply Highly Multiplexed, Quantitative Peptide Assays to Measure Cancer-Relevant Proteins in Plasma

Abbatiello, S. E., Schilling, B., Mani, D. R., Zimmerman, L. J., Hall, S. C., MacLean, B., Albertolle, M., Allen, S., Burgess, M., Cusack, M. P., et al. (2015) · Molecular & Cellular Proteomics · doi:10.1074/mcp.m114.047050 · Cited by 215 · see citing works

An open letter to our readers on the use of antibodies

Saper, C. B. (2005) · The Journal of Comparative Neurology · doi:10.1002/cne.20839 · Cited by 188 · see citing works

Cross-reactivity in antibody microarrays and multiplexed sandwich assays: shedding light on the dark side of multiplexing

Juncker, D., Bergeron, S., Laforte, V., & Li, H. (2013) · Current Opinion in Chemical Biology · doi:10.1016/j.cbpa.2013.11.012 · Cited by 145 · see citing works

Suitability of muscarinic acetylcholine receptor antibodies for immunohistochemistry evaluated on tissue sections of receptor gene-deficient mice

Jositsch, G., Papadakis, T., Haberberger, R. V., Wolff, M., Wess, J., & Kummer, W. (2008) · Naunyn-Schmiedeberg s Archives of Pharmacology · doi:10.1007/s00210-008-0365-9 · Cited by 143 · see citing works

Antibody anarchy: A call to order

Baker, M. (2015) · Nature · doi:10.1038/527545a · Cited by 140 · see citing works

Assessment of a method to characterize antibody selectivity and specificity for use in immunoprecipitation

Marcon, E., Jain, H., Bhattacharya, A., Guo, H., Phanse, S., Pu, S., Byram, G., Collins, B. C., Dowdell, E., Fenner, M., et al. (2015) · Nature Methods · doi:10.1038/nmeth.3472 · Cited by 127 · see citing works

Fine-Tuning Next-Generation Genome Editing Tools

Kanchiswamy, C. N., Maffei, M. E., Malnoy, M., Velasco, R., & Kim, J. (2016) · Trends in biotechnology · doi:10.1016/j.tibtech.2016.03.007 · Cited by 68 · see citing works

Exploration of high-density protein microarrays for antibody validation and autoimmunity profiling

Sjöberg, R., Mattsson, C., Andersson, E., Hellström, C., Uhlén, M., Schwenk, J. M., Ayoglu, B., & Nilsson, P. (2015) · New Biotechnology · doi:10.1016/j.nbt.2015.09.002 · Cited by 57 · see citing works

Minimum information about a protein affinity reagent (MIAPAR)

Bourbeillon, J., Orchard, S. E., Benhar, I., Borrebaeck, C. A. K., de Daruvar, A., Dübel, S., Frank, R., Gibson, F., Gloriam, D. E. I., Haslam, N. J., et al. (2010) · Nature Biotechnology · doi:10.1038/nbt0710-650 · Cited by 54 · see citing works

Comparative assessment of fluorescent transgene methods for quantitative imaging in human cells

Mahen, R., Koch, B., Wachsmuth, M., Politi, A. Z., Perez‐Gonzalez, A., Mergenthaler, J., Cai, Y., & Ellenberg, J. (2014) · Molecular Biology of the Cell · doi:10.1091/mbc.e14-06-1091 · Cited by 50 · see citing works

Systematic validation of antibody binding and protein subcellular localization using siRNA and confocal microscopy

Stadler, C., Hjelmare, M., Neumann, B., Jonasson, K., Pepperkok, R., Uhlén, M., & Lundberg, E. K. (2012) · Journal of Proteomics · doi:10.1016/j.jprot.2012.01.030 · Cited by 37 · see citing works

The Resource Identification Initiative: A Cultural Shift in Publishing

Initiative, R. R. I., Bandrowski, A. E., Brush, M., Grethe, J. S., Haendel, M. A., Kennedy, D. N., Hill, S., Hof, P. R., Martone, M. E., Pols, M. S., et al. (2015) · Neuroinformatics · doi:10.1007/s12021-015-9284-3 · Cited by 34 · see citing works

Parallel Protein Detection by Solid‐Phase Proximity Ligation Assay with Real‐Time PCR or Sequencing

Ebai, T., Kamali‐Moghaddam, M., & Landegren, U. D. (2015) · Current Protocols in Molecular Biology · doi:10.1002/0471142727.mb2010s109 · Cited by 28 · see citing works

siRNA knockdown validation 101: Incorporating negative controls in antibody research

Olds, W., & Li, J. (2016) · F1000Research · doi:10.12688/f1000research.8159.1 · Cited by 6 · see citing works

The human protein atlas: a spatial map of the human proteome

Thul, P. J., & Lindskog, C. (2018) · Protein Science · doi:10.1002/pro.3307 · Cited by 1,299 · see citing works

The protein expression profile of ACE2 in human tissues

Hikmet, F., Méar, L., Edvinsson, Å., Micke, P., Uhlén, M., & Lindskog, C. (2020) · Molecular Systems Biology · doi:10.15252/msb.20209610 · Cited by 1,016 · see citing works

Genetics meets proteomics: perspectives for large population-based studies

Suhre, K., McCarthy, M. I., & Schwenk, J. M. (2021) · Nature Reviews Genetics · doi:10.1038/s41576-020-0268-2 · Cited by 478 · see citing works

A high-stringency blueprint of the human proteome

Adhikari, S., Nice, E. C., Deutsch, E. W., Lane, L., Omenn, G. S., Pennington, S. R., Paik, Y.-K., Overall, C. M., Corrales, F. J., Cristea, I. M., et al. (2020) · Nature Communications · doi:10.1038/s41467-020-19045-9 · Cited by 210 · see citing works

Double-Cone Localization and Seasonal Expression Pattern Suggest a Role in Magnetoreception for European Robin Cryptochrome 4

Günther, A., Einwich, A., Sjulstok, E., Feederle, R., Bolte, P., Koch, K.-W., Solov’yov, I. A., & Mouritsen, H. (2018) · Current Biology · doi:10.1016/j.cub.2017.12.003 · Cited by 204 · see citing works

Implementation of an antibody characterization procedure and application to the major ALS/FTD disease gene C9ORF72

Laflamme, C., McKeever, P. M., Kumar, R., Schwartz, J., Kolahdouzan, M., Chen, C. X., You, Z., Benaliouad, F., Gileadi, O., McBride, H. M., et al. (2019) · eLife · doi:10.7554/elife.48363 · Cited by 155 · see citing works

A multiregional proteomic survey of the postnatal human brain

Carlyle, B. C., Kitchen, R. R., Kanyo, J. E., Voss, E. Z., Pletikos, M., Sousa, A. M. M., Lam, T. T., Gerstein, M. B., Sestan, N., & Nairn, A. C. (2017) · Nature Neuroscience · doi:10.1038/s41593-017-0011-2 · Cited by 143 · see citing works

Reproducibility2020: Progress and priorities

Freedman, L. P., Venugopalan, G., & Wisman, R. (2017) · F1000Research · doi:10.12688/f1000research.11334.1 · Cited by 102 · see citing works

Targeting RAS-driven human cancer cells with antibodies to upregulated and essential cell-surface proteins

Martinko, A. J., Truillet, C., Julien, O., Diaz, J. E., Horlbeck, M. A., Whiteley, G., Blonder, J., Weissman, J. S., Bandyopadhyay, S., Evans, M. J., et al. (2018) · eLife · doi:10.7554/elife.31098 · Cited by 91 · see citing works

Disrupted hippocampal growth hormone secretagogue receptor 1α interaction with dopamine receptor D1 plays a role in Alzheimer′s disease

Tian, J., Guo, L., Sui, S., Driskill, C., Phensy, A., Wang, Q., Gauba, E., Zigman, J. M., Swerdlow, R. H., Kroener, S., et al. (2019) · Science Translational Medicine · doi:10.1126/scitranslmed.aav6278 · Cited by 72 · see citing works

SALL4 controls cell fate in response to DNA base composition

Pantier, R., Chhatbar, K., Quante, T., Skourti-Stathaki, K., Cholewa-Waclaw, J., Alston, G., Alexander-Howden, B., Lee, H. Y., Cook, A. G., Spruijt, C. G., et al. (2021) · Molecular Cell · doi:10.1016/j.molcel.2020.11.046 · Cited by 45 · see citing works

Scaling of an antibody validation procedure enables quantification of antibody performance in major research applications

Ayoubi, R., Ryan, J., Biddle, M. S., Alshafie, W., Fotouhi, M., Bolivar, S. G., Ruiz Moleon, V., Eckmann, P., Worrall, D., McDowell, I., et al. (2023) · eLife · doi:10.7554/elife.91645.2 · Cited by 53 · see citing works

Enhancing reproducibility: Failures from Reproducibility Initiatives underline core challenges

Mullane, K., & Williams, M. (2017) · Biochemical Pharmacology · doi:10.1016/j.bcp.2017.04.008 · Cited by 28 · see citing works

Measuring expression heterogeneity of single-cell cytoskeletal protein complexes

Vlassakis, J., Hansen, L. L., Higuchi-Sanabria, R., Zhou, Y., Tsui, C. K., Dillin, A., Huang, H., & Herr, A. E. (2020) · doi:10.1101/2020.09.12.294801 · Cited by 16 · see citing works

Addressing selective reporting of experiments through predefined exclusion criteria

Neves, K., & Amaral, O. B. (2020) · eLife · doi:10.7554/elife.56626 · Cited by 15 · see citing works

The Role of Apoptosis and Autophagy in the Hypothalamic-Pituitary-Adrenal (HPA) Axis After Traumatic Brain Injury (TBI)

Taheri, S., Karaca, Z., Mehmetbeyoglu, E., Hamurcu, Z., Yılmaz, Z., Dal, F., Cınar, V., Ulutabanca, H., Tanriverdi, F., Unluhizarci, K., et al. (2022) · doi:10.21203/rs.3.rs-1159219/v1 · Cited by 26 · see citing works

DETERMINING QUALITY REQUIREMENTS AT THE UNIVERSITIES TO IMPROVE THE QUALITY OF EDUCATION

Mustafa, N. H., Kelesbayev, D., & Alibekova, Z. D. (2018) · The Journal of Economic Research & Business Administration · doi:10.26577/be-2018-2-2141

Susquehanna Chorale Spring Concert "Roots and Wings"

Hawkins, D. (2017)

Using DataGrid Control to Realize DataBase of Querying in VB6.0

Hu, S. (2000) · Modern Computer

Study and Two Types of Typical Usage of DataGrid Web Server Control

Hai-tao, L. (2005) · Modern Computer

Achieving Parameter of DBSCAN Based on Datagrid

Xu, Q. (2010)