The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.
A general method to fine-tune fluorophores for live-cell and in vivo imaging
Open accessAbstract
Pushing the frontier of fluorescence microscopy requires the design of enhanced fluorophores with finely tuned properties. We recently discovered that incorporation of four-membered azetidine rings into classic fluorophore structures elicits substantial increases in brightness and photostability, resulting in the Janelia Fluor (JF) series of dyes. We refined and extended this strategy, finding that incorporation of 3-substituted azetidine groups allows rational tuning of the spectral and chemical properties of rhodamine dyes with unprecedented precision. This strategy allowed us to establish principles for fine-tuning the properties of fluorophores and to develop a palette of new fluorescent and fluorogenic labels with excitation ranging from blue to the far-red. Our results demonstrate the versatility of these new dyes in cells, tissues and animals.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Fiji: an open-source platform for biological-image analysis
Schindelin, J., Arganda-Carreras, I., Frise, E., Kaynig, V., Longair, M., Pietzsch, T., Preibisch, S., Rueden, C., Saalfeld, S., Schmid, B., et al. (2012) · Nature Methods · doi:10.1038/nmeth.2019 · Cited by 66,890
Ultrasensitive fluorescent proteins for imaging neuronal activity
Chen, T.-W., Wardill, T. J., Sun, Y., Pulver, S. R., Renninger, S. L., Baohan, A., Schreiter, E. R., Kerr, R. A., Orger, M. B., Jayaraman, V., et al. (2013) · Nature · doi:10.1038/nature12354 · Cited by 6,474
A multilevel multimodal circuit enhances action selection in drosophila
Ohyama, T., Schneider-Mizell, C. M., Fetter, R. D., Aleman, J. V., Franconville, R., Rivera-Alba, M., Mensh, B. D., Branson, K. M., Simpson, J. H., Truman, J. W., et al. (2015) · Nature · doi:10.1038/nature14297 · Cited by 502
Cited by
Deep learning for cellular image analysis
Moen, E., Bannon, D., Kudo, T., Graf, W., Covert, M., & Van Valen, D. (2019) · Nature Methods · doi:10.1038/s41592-019-0403-1 · Cited by 1,225
Wang, C., Chi, W., Qiao, Q., Tan, D., Xu, Z., & Liu, X. (2021) · Chemical Society Reviews · doi:10.1039/d1cs00239b · Cited by 710
Bright and photostable chemigenetic indicators for extended in vivo voltage imaging
Abdelfattah, A. S., Kawashima, T., Singh, A., Novak, O., Liu, H., Shuai, Y., Huang, Y.-C., Campagnola, L., Seeman, S. C., Yu, J., et al. (2019) · Science · doi:10.1126/science.aav6416 · Cited by 455
Self-citationA general strategy to develop cell permeable and fluorogenic probes for multicolour nanoscopy
Wang, L., Tran, M., D’Este, E., Roberti, J., Koch, B., Xue, L., & Johnsson, K. (2020) · Nature Chemistry · doi:10.1038/s41557-019-0371-1 · Cited by 382
A Host-Produced Quorum-Sensing Autoinducer Controls a Phage Lysis-Lysogeny Decision
Silpe, J. E., & Bassler, B. L. (2019) · Cell · doi:10.1016/j.cell.2018.10.059 · Cited by 363
Metadata
- Authors
- Grimm, J. B., Muthusamy, A. K., Liang, Y., Brown, T. A., Lemon, W. C., Patel, R., Lu, R., Macklin, J. J., Keller, P. J., Ji, N., & Lavis, L. D.
- Venue
- Nature Methods
- Published
- 2017-09-04
- Volume
- 14
- Issue
- 10
- Pages
- 987-994
- ISSN
- 1548-7091 (ISSN-L)
- 1548-7105
- OpenAlex
- W2605776475
- Cited by
- 665
- References
- 3
- FWCI
- 31.47
- Type
- journal article
- Language
- en
- Open access
- green
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: A general method to fine-tune fluorophores for live-cell and in vivo imaging. https://app.cochat.ai/papers/10.1038/nmeth.4403
- The paper
- Grimm, J. B., Muthusamy, A. K., Liang, Y., Brown, T. A., Lemon, W. C., Patel, R., Lu, R., Macklin, J. J., Keller, P. J., Ji, N., & Lavis, L. D. (2017). A general method to fine-tune fluorophores for live-cell and in vivo imaging. Nature Methods. https://doi.org/10.1038/nmeth.4403
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
