In situ detection of miRNAs in animal embryos using LNA-modified oligonucleotide probes
Abstract
MicroRNAs (miRNAs) are 20-23 nucleotide (nt) RNA molecules that regulate gene expression post-transcriptionally. A key step toward understanding the function of the hundreds of miRNAs identified in animals is to determine their expression during development. Here we performed a detailed analysis of conditions for in situ detection of miRNAs in the zebrafish embryo using locked nucleic acid (LNA)-modified DNA probes and report expression patterns for 15 miRNAs in the mouse embryo.
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LNA probes with two or more different internal nucleotides detect signal from a single miRNA and not others with similar sequence.
“In contrast, two mismatches in the miR-181a probe are sufficient to eliminate specific in situ hybridization signal, supporting the conclusion that probes with two or more different internal nucleotides detect signal from a single miRNA and not others with similar sequence [36].”
From MicroRNAs show a wide diversity of expression profiles in the developing and mature central nervous system (2007) · cites this paper as [36]
Cites
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LNA (Locked Nucleic Acid): High-Affinity Targeting of Complementary RNA and DNA
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A Mammalian microRNA Expression Atlas Based on Small RNA Library Sequencing
Landgraf, P., Rusu, M., Sheridan, R., Sewer, A., Iovino, N., Aravin, A., Pfeffer, S., Rice, A., Kamphorst, A. O., Landthaler, M., et al. (2007) · Cell · doi:10.1016/j.cell.2007.04.040 · Cited by 3,626 · see citing works
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MicroRNA expression in the adult mouse central nervous system
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Self-citationMicroRNA Expression Is Required for Pancreatic Islet Cell Genesis in the Mouse
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MicroRNA-183 family members regulate sensorineural fates in the inner ear
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- Authors
- Kloosterman, W. P., Wienholds, E., de Bruijn, E., Kauppinen, S., & Plasterk, R. H. A.
- Venue
- Nature Methods
- Published
- 2005-12-20
- Volume
- 3
- Issue
- 1
- Pages
- 27-29
- ISSN
- 1548-7091
- 1548-7105
- Cited by
- 540
- References
- 2
- Language
- en
- Licence
- www.springer.com/tdm (tdm)
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