Cited by 405Neuroscience and Neuropharmacology ResearchCellular transport and secretionIon channel regulation and function

The DOI matches its Crossref record; 56 of the 56 references it cites were found in a registry or an index.

Release probability of hippocampal glutamatergic terminals scales with the size of the active zone

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Holderith, Noemi, Lorincz, Andrea, Katona, Gergely, Rózsa, Balázs, Kulik, Akos, Watanabe, Masahiko, Nusser, Zoltan·2012·Nature Neuroscience

Abstract

Cortical synapses have structural, molecular and functional heterogeneity; our knowledge regarding the relationship between their ultrastructural and functional parameters is still fragmented. Here we asked how the neurotransmitter release probability and presynaptic [Ca(2+)] transients relate to the ultrastructure of rat hippocampal glutamatergic axon terminals. Two-photon Ca(2+) imaging-derived optical quantal analysis and correlated electron microscopic reconstructions revealed a tight correlation between the release probability and the active-zone area. Peak amplitude of [Ca(2+)] transients in single boutons also positively correlated with the active-zone area. Freeze-fracture immunogold labeling revealed that the voltage-gated calcium channel subunit Cav2.1 and the presynaptic protein Rim1/2 are confined to the active zone and their numbers scale linearly with the active-zone area. Gold particles labeling Cav2.1 were nonrandomly distributed in the active zones. Our results demonstrate that the numbers of several active-zone proteins, including presynaptic calcium channels, as well as the number of docked vesicles and the release probability, scale linearly with the active-zone area.

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Metadata

Authors
Holderith, N., Lorincz, A., Katona, G., Rózsa, B., Kulik, A., Watanabe, M., & Nusser, Z.
Affiliations
  • HUN-REN Institute of Experimental Medicine (HU)
  • Hokkaido University (JP)
Venue
Nature Neuroscience
Published
2012-06-10
Volume
15
Issue
7
Pages
988-997
ISSN
  • 1097-6256 (ISSN-L)
  • 1546-1726
OpenAlex
W2093514926
Cited by
405
References
56
Citations by year
2026: 3 · 2025: 15 · 2024: 29 · 2023: 29 · 2022: 30 · 2021: 32 · 2020: 38 · 2019: 28 · 2018: 39 · 2017: 24 · 2016: 30 · 2015: 36 · 2014: 34 · 2013: 32 · 2012: 6
FWCI
14.24
Type
journal article
Language
en
Update notices
erratum (2015-12-29)
Open access
Locations
  • Nature Neuroscience (publishedVersion)
  • PubMed (publishedVersion)
  • Repository of the Academy's Library (Library of the Hungarian Academy of Sciences) (acceptedVersion)
  • Europe PMC (PubMed Central) (acceptedVersion)
  • PubMed Central (acceptedVersion)
Funders
Wellcome Trust (083484, 090197, 094513, 083484/Z/07/Z, 090197/Z/09/Z, 094513/Z/10/Z)
Topics
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ion channel regulation and function
Keywords
release probability, active zone, glutamatergic synapses, CA3 pyramidal cells, voltage-gated calcium channels, Cav2.1, docked vesicles, synaptic ultrastructure, two-photon calcium imaging
MeSH terms
Action Potentials, Animals, Hippocampus, Male, Random Allocation, Synaptic Vesicles, Rats, Wistar, Presynaptic Terminals, Calcium Channels, N-Type, Rats

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