The DOI matches its Crossref record; 7 of the 7 references it cites were found in a registry or an index.
Synergistic binding of transcription factors to cell-specific enhancers programs motor neuron identity
Open accessAbstract
Efficient transcriptional programming promises to open new frontiers in regenerative medicine. However, mechanisms by which programming factors transform cell fate are unknown, preventing more rational selection of factors to generate desirable cell types. Three transcription factors, Ngn2, Isl1 and Lhx3, were sufficient to program rapidly and efficiently spinal motor neuron identity when expressed in differentiating mouse embryonic stem cells. Replacement of Lhx3 by Phox2a led to specification of cranial, rather than spinal, motor neurons. Chromatin immunoprecipitation–sequencing analysis of Isl1, Lhx3 and Phox2a binding sites revealed that the two cell fates were programmed by the recruitment of Isl1-Lhx3 and Isl1-Phox2a complexes to distinct genomic locations characterized by a unique grammar of homeodomain binding motifs. Our findings suggest that synergistic interactions among transcription factors determine the specificity of their recruitment to cell type–specific binding sites and illustrate how a single transcription factor can be repurposed to program different cell types.
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Cites
Direct conversion of fibroblasts to functional neurons by defined factors
Vierbuchen, T., Ostermeier, A., Pang, Z. P., Kokubu, Y., Südhof, T. C., & Wernig, M. (2010) · Nature · doi:10.1038/nature08797 · Cited by 3,017
Neuronal specification in the spinal cord: inductive signals and transcriptional codes
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A map of the cis-regulatory sequences in the mouse genome
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Direct conversion of mouse fibroblasts to hepatocyte-like cells by defined factors
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Variation in Homeodomain DNA Binding Revealed by High-Resolution Analysis of Sequence Preferences
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Cited by
CTCF establishes discrete functional chromatin domains at the Hox clusters during differentiation
Narendra, V., Rocha, P. P., An, D., Raviram, R., Skok, J. A., Mazzoni, E. O., & Reinberg, D. (2015) · Science · doi:10.1126/science.1262088 · Cited by 535
Self-citationTranscription factor-mediated differentiation of human iPSCs into neurons
Fernandopulle, M. S., Prestil, R., Grunseich, C., Wang, C., Gan, L., & Ward, M. E. (2018) · Current Protocols in Cell Biology · doi:10.1002/cpcb.51 · Cited by 461
Absence of a simple code: how transcription factors read the genome
Slattery, M., Zhou, T., Yang, L., Dantas Machado, A. C., Gordân, R., & Rohs, R. (2014) · Trends in Biochemical Sciences · doi:10.1016/j.tibs.2014.07.002 · Cited by 537
Establishing neuronal diversity in the spinal cord: a time and a place
Sagner, A., & Briscoe, J. (2019) · Development · doi:10.1242/dev.182154 · Cited by 281
Loss of motoneuron-specific microRNA-218 causes systemic neuromuscular failure
Amin, N. D., Bai, G., Klug, J. R., Bonanomi, D., Pankratz, M. T., Gifford, W. D., Hinckley, C. A., Sternfeld, M. J., Driscoll, S. P., Dominguez, B., et al. (2015) · Science · doi:10.1126/science.aad2509 · Cited by 154
Metadata
- Authors
- Mazzoni, E. O., Mahony, S., Closser, M., Morrison, C. A., Nedelec, S., Williams, D. J., An, D., Gifford, D. K., & Wichterle, H.
- Venue
- Nature Neuroscience
- Published
- 2013-07-21
- Volume
- 16
- Issue
- 9
- Pages
- 1219-1227
- ISSN
- 1097-6256 (ISSN-L)
- 1546-1726
- OpenAlex
- W1561734908
- Cited by
- 229
- References
- 7
- FWCI
- 7.93
- Type
- journal article
- Language
- en
- Open access
- green
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- Mazzoni, E. O., Mahony, S., Closser, M., Morrison, C. A., Nedelec, S., Williams, D. J., An, D., Gifford, D. K., & Wichterle, H. (2013). Synergistic binding of transcription factors to cell-specific enhancers programs motor neuron identity. Nature Neuroscience. https://doi.org/10.1038/nn.3467
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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