The DOI matches its Crossref record; 27 of the 28 references it cites were found in a registry or an index.
Direct imaging of single-walled carbon nanotubes in cells
Abstract
The development of single-walled carbon nanotubes for various biomedical applications is an area of great promise. However, the contradictory data on the toxic effects of single-walled carbon nanotubes highlight the need for alternative ways to study their uptake and cytotoxic effects in cells. Single-walled carbon nanotubes have been shown to be acutely toxic in a number of types of cells, but the direct observation of cellular uptake of single-walled carbon nanotubes has not been demonstrated previously due to difficulties in discriminating carbon-based nanotubes from carbon-rich cell structures. Here we use transmission electron microscopy and confocal microscopy to image the translocation of single-walled carbon nanotubes into cells in both stained and unstained human cells. The nanotubes were seen to enter the cytoplasm and localize within the cell nucleus, causing cell mortality in a dose-dependent manner.
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“For example, MWCNTs have high toxicity and hydrophobicity [562]; therefore CNT/SMP have to be subjected to stringent evaluation before biomedical applications.”
From A review of stimuli-responsive shape memory polymer composites (2013) · cites this paper as [562]
In the citing paper
“For example, MWCNTs have high toxicity and hydrophobicity [562]; therefore CNT/SMP have to be subjected to stringent evaluation before biomedical applications.”
From A review of stimuli-responsive shape memory polymer composites (2013) · cites this paper as [562]
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Cited by
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Metadata
- Authors
- Porter, A. E., Gass, M., Muller, K., Skepper, J. N., Midgley, P. A., & Welland, M.
- Affiliations
- University of Cambridge (GB)
- Daresbury Laboratory (GB)
- Corresponding author
- Porter, Alexandra E.
- Venue
- Nature Nanotechnology
- Published
- 2007-10-28
- Volume
- 2
- Issue
- 11
- Pages
- 713-717
- ISSN
- 1748-3387 (ISSN-L)
- 1748-3395
- OpenAlex
- W2037122021
- Cited by
- 564
- References
- 28
- Citations by year
- 2026: 1 · 2025: 5 · 2024: 14 · 2023: 10 · 2022: 22 · 2021: 8 · 2020: 14 · 2019: 19 · 2018: 65 · 2017: 28 · 2016: 34 · 2015: 31 · 2014: 44 · 2013: 47 · 2012: 54
- FWCI
- 28.92
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Nature Nanotechnology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Wellcome Trust
- Leverhulme Trust
- Engineering and Physical Sciences Research Council (EP/D040396/1)
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › Graphene and Nanomaterials Applications
- Physical Sciences › Materials Science › Materials Chemistry › Nanoparticles: synthesis and applications
- Physical Sciences › Materials Science › General Materials Science › Nanotechnology research and applications
- Keywords
- single-walled carbon nanotubes, cellular uptake, cytotoxicity, nuclear localization, dose-dependent cell death, nanotoxicology
- MeSH terms
- Cells, Cultured, Humans, Macrophages, Phagocytosis, Microscopy, Confocal, Nanotubes, Carbon, Microscopy, Electron, Transmission
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