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Three-dimensional tissue culture based on magnetic cell levitation
Open accessAbstract
Cell culture is an essential tool for drug discovery, tissue engineering, and stem cell research. Conventional tissue culture produces two-dimensional (2D) cell growth with gene expression, signaling, and morphology that can differ from those in vivo and thus compromise clinical relevancy1–5. Here we report a three-dimensional (3D) culture of cells based on magnetic levitation in the presence of hydrogels containing gold and magnetic iron oxide (MIO) nanoparticles plus filamentous bacteriophage. This methodology allows for control of cell mass geometry and guided, multicellular clustering of different cell types in co-culture through spatial variance of the magnetic field. Moreover, magnetic levitation of human glioblastoma cells demonstrates similar protein expression profiles to those observed in human tumor xenografts. Taken together, these results suggest levitated 3D culture with magnetized phage-based hydrogels more closely recapitulates in vivo protein expression and allows for long-term multi-cellular studies.
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Designing materials for biology and medicine
Langer, R., & Tirrell, D. A. (2004) · Nature · doi:10.1038/nature02388 · Cited by 3,198
Taking Cell-Matrix Adhesions to the Third Dimension
Cukierman, E., Pankov, R., Stevens, D. R., & Yamada, K. M. (2001) · Science · doi:10.1126/science.1064829 · Cited by 3,027
The third dimension bridges the gap between cell culture and live tissue
Pampaloni, F., Reynaud, E. G., & Stelzer, E. H. K. (2007) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm2236 · Cited by 2,817
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Is it time to start transitioning from 2D to 3D cell culture?
Jensen, C., & Teng, Y. (2020) · Frontiers in Molecular Biosciences · doi:10.3389/fmolb.2020.00033 · Cited by 1,640
Three-dimensional in vitro cell culture models in drug discovery and drug repositioning
Langhans, S. A. (2018) · Frontiers in Pharmacology · doi:10.3389/fphar.2018.00006 · Cited by 1,522
Nanotechnological strategies for engineering complex tissues
Dvir, T., Timko, B. P., Kohane, D. S., & Langer, R. (2011) · Nature Nanotechnology · doi:10.1038/nnano.2010.246 · Cited by 1,367
3D tumor spheroids: an overview on the tools and techniques used for their analysis
Costa, E. C., Moreira, A. F., de Melo-Diogo, D., Gaspar, V. M., Carvalho, M. P., & Correia, I. J. (2016) · Biotechnology Advances · doi:10.1016/j.biotechadv.2016.11.002 · Cited by 929
Three-dimensional culture systems in cancer research: Focus on tumor spheroid model
Nath, S., & Devi, G. R. (2016) · Pharmacology & Therapeutics · doi:10.1016/j.pharmthera.2016.03.013 · Cited by 919
Metadata
- Authors
- Souza, G. R., Molina, J. R., Raphael, R. M., Ozawa, M. G., Stark, D. J., Levin, C. S., Bronk, L. F., Ananta, J. S., Mandelin, J., Georgescu, M.-M., Bankson, J. A., Gelovani, J. G., Killian, T. C., Arap, W., & Pasqualini, R.
- Venue
- Nature Nanotechnology
- Published
- 2010-03-14
- Volume
- 5
- Issue
- 4
- Pages
- 291-296
- ISSN
- 1748-3387 (ISSN-L)
- 1748-3395
- OpenAlex
- W2122920174
- Cited by
- 662
- References
- 3
- FWCI
- 16.38
- Type
- journal article
- Language
- en
- Open access
- green
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- Souza, G. R., Molina, J. R., Raphael, R. M., Ozawa, M. G., Stark, D. J., Levin, C. S., Bronk, L. F., Ananta, J. S., Mandelin, J., Georgescu, M.-M., Bankson, J. A., Gelovani, J. G., Killian, T. C., Arap, W., & Pasqualini, R. (2010). Three-dimensional tissue culture based on magnetic cell levitation. Nature Nanotechnology. https://doi.org/10.1038/nnano.2010.23
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