The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.
Bidirectional cross-kingdom RNAi and fungal uptake of external RNAs confer plant protection
Open accessAbstract
Aggressive fungal pathogens such as Botrytis and Verticillium spp. cause severe crop losses worldwide. We recently discovered that Botrytis cinerea delivers small RNAs (Bc–sRNAs) into plant cells to silence host immunity genes. Such sRNA effectors are mostly produced by Botrytis cinerea Dicer-like protein 1 (Bc-DCL1) and Bc-DCL2. Here we show that expressing sRNAs that target Bc-DCL1 and Bc-DCL2 in Arabidopsis and tomato silences Bc-DCL genes and attenuates fungal pathogenicity and growth, exemplifying bidirectional cross-kingdom RNAi and sRNA trafficking between plants and fungi. This strategy can be adapted to simultaneously control multiple fungal diseases. We also show that Botrytis can take up external sRNAs and double-stranded RNAs (dsRNAs). Applying sRNAs or dsRNAs that target Botrytis DCL1 and DCL2 genes on the surface of fruits, vegetables and flowers significantly inhibits grey mould disease. Such pathogen gene-targeting RNAs represent a new generation of environmentally friendly fungicides.
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Mao, Y.-B., Cai, W.-J., Wang, J.-W., Hong, G.-J., Tao, X.-Y., Wang, L.-J., Huang, Y.-P., & Chen, X.-Y. (2007) · Nature Biotechnology · doi:10.1038/nbt1352 · Cited by 1,358
Buck, A. H., Coakley, G., Simbari, F., McSorley, H. J., Quintana, J. F., Le Bihan, T., Kumar, S., Abreu-Goodger, C., Lear, M., Harcus, Y., et al. (2014) · Nature Communications · doi:10.1038/ncomms6488 · Cited by 770
Bolognesi, R., Ramaseshadri, P., Anderson, J., Bachman, P., Clinton, W., Flannagan, R., Ilagan, O., Lawrence, C., Levine, S., Moar, W., et al. (2012) · PLoS ONE · doi:10.1371/journal.pone.0047534 · Cited by 473
Cited by
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Fisher, M. C., Hawkins, N. J., Sanglard, D., & Gurr, S. J. (2018) · Science · doi:10.1126/science.aap7999 · Cited by 1,555
Tackling the emerging threat of antifungal resistance to human health
Fisher, M. C., Alastruey-Izquierdo, A., Berman, J., Bicanic, T., Bignell, E. M., Bowyer, P., Bromley, M., Brüggemann, R., Garber, G., Cornely, O. A., et al. (2022) · Nature Reviews Microbiology · doi:10.1038/s41579-022-00720-1 · Cited by 1,179
Control of postharvest fungal diseases in fruits using external application of RNAi
de Oliveira Filho, J. G., Silva, G. D. C., Cipriano, L., Gomes, M., & Egea, M. B. (2021) · Journal of Food Science · doi:10.1111/1750-3841.15816 · Cited by 37
Melet, L., Canan, J., Villalobos, P., Vidal-Veuthey, B., González-Toro, F., Orellana, I., Rivas-Pardo, J. A., Cárdenas, J. P., Moraga, C., Castro-Fernández, V., et al. (2025) · Computational and Structural Biotechnology Journal · doi:10.1016/j.csbj.2025.11.009 · Cited by 1
Metadata
- Authors
- Wang, M., Weiberg, A., Lin, F.-M., Thomma, B. P. H. J., Huang, H.-D., & Jin, H.
- Venue
- Nature Plants
- Published
- 2016-09-19
- Volume
- 2
- Issue
- 10
- Pages
- 16151-16151
- ISSN
- 2055-026X (ISSN-L)
- 2055-0278
- OpenAlex
- W2521150917
- Cited by
- 801
- References
- 3
- FWCI
- 49.52
- Type
- journal article
- Language
- en
- Open access
- green
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- Wang, M., Weiberg, A., Lin, F.-M., Thomma, B. P. H. J., Huang, H.-D., & Jin, H. (2016). Bidirectional cross-kingdom RNAi and fungal uptake of external RNAs confer plant protection. Nature Plants. https://doi.org/10.1038/nplants.2016.151
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