Characterizing the mechanism of action of double-stranded RNA activity against western corn rootworm (Diabrotica virgifera virgifera LeConte)
Open accessAbstract
RNA interference (RNAi) has previously been shown to be effective in western corn rootworm (WCR, Diabrotica virgifera virgifera LeConte) larvae via oral delivery of synthetic double-stranded RNA (dsRNA) in an artificial diet bioassay, as well as by ingestion of transgenic corn plant tissues engineered to express dsRNA. Although the RNAi machinery components appear to be conserved in Coleopteran insects, the key steps in this process have not been reported for WCR. Here we characterized the sequence of events that result in mortality after ingestion of a dsRNA designed against WCR larvae. We selected the Snf7 ortholog (DvSnf7) as the target mRNA, which encodes an essential protein involved in intracellular trafficking. Our results showed that dsRNAs greater than or equal to approximately 60 base-pairs (bp) are required for biological activity in artificial diet bioassays. Additionally, 240 bp dsRNAs containing a single 21 bp match to the target sequence were also efficacious, whereas 21 bp short interfering (si) RNAs matching the target sequence were not. This result was further investigated in WCR midgut tissues: uptake of 240 bp dsRNA was evident in WCR midgut cells while a 21 bp siRNA was not, supporting the size-activity relationship established in diet bioassays. DvSnf7 suppression was observed in a time-dependent manner with suppression at the mRNA level preceding suppression at the protein level when a 240 bp dsRNA was fed to WCR larvae. DvSnf7 suppression was shown to spread to tissues beyond the midgut within 24 h after dsRNA ingestion. These events (dsRNA uptake, target mRNA and protein suppression, systemic spreading, growth inhibition and eventual mortality) comprise the overall mechanism of action by which DvSnf7 dsRNA affects WCR via oral delivery and provides insights as to how targeted dsRNAs in general are active against insects.
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Suppression of Snf7 causes increased D. v. virgifera larval mortality leading to reduced root damage.
“Suppression of Snf7 has been reported to cause increased D. v. virgifera larval mortality leading to reduced root damage (Bolognesi et al., 2012).”
From Genetically engineered crops help support conservation biological control (2019) · cites this paper as Bolognesi et al., 2012
The GE maize event MON87411 produces a dsRNA targeting the Snf7 protein in D. v. virgifera, which is crucial for the transport of transmembrane proteins.
“This GE maize event (MON87411) produces a dsRNA targeting the Snf7 protein in D. v. virgifera, which is crucial for the transport of transmembrane proteins (Bachman et al., 2013;Bolognesi et al., 2012).”
From Genetically engineered crops help support conservation biological control (2019) · cites this paper as Bolognesi et al., 2012
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Cites
Proceedings of the National Academy of Sciences of the United States of America
Dowling, J. E., & Wald, G. (2009) · Nutrition Reviews · doi:10.1111/j.1753-4887.1981.tb06752.x · Cited by 17,937 · see citing works
Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans
Fire, A., Xu, S., Montgomery, M. K., Kostas, S. A., Driver, S. E., & Mello, C. C. (1998) · Nature · doi:10.1038/35888 · Cited by 15,429 · see citing works
Control of coleopteran insect pests through RNA interference
Baum, J. A., Bogaert, T., Clinton, W., Heck, G. R., Feldmann, P., Ilagan, O., Johnson, S., Plaetinck, G., Munyikwa, T., Pleau, M., et al. (2007) · Nature Biotechnology · doi:10.1038/nbt1359 · Cited by 1,789 · see citing works
Mao, Y.-B., Cai, W.-J., Wang, J.-W., Hong, G.-J., Tao, X.-Y., Wang, L.-J., Huang, Y.-P., & Chen, X.-Y. (2007) · Nature Biotechnology · doi:10.1038/nbt1352 · Cited by 1,358 · cited 1× · see citing works
“economically important pests, including western corn rootworm (WCR, Diabrotica virgifera virgifera), southern corn rootworm (SCR, Diabrotica undecimpunctata howardii), Colorado potato beetle (Leptinotarsa decemlineata) [7], cotton bollworm (Helicoverpa armigera) [9], beet armyworm (Spodoptera exigua) [10] and brown pl…”
BackgroundGene silencing as an adaptive defence against viruses
Waterhouse, P. M., Wang, M., & Lough, T. J. (2001) · Nature · doi:10.1038/35081168 · Cited by 986 · see citing works
Mechanisms of dsRNA uptake in insects and potential of RNAi for pest control: A review
Huvenne, H., & Smagghe, G. J. (2009) · Journal of Insect Physiology · doi:10.1016/j.jinsphys.2009.10.004 · Cited by 966 · see citing works
Terenius, O., Papanicolaou, A., Garbutt, J. S., Eleftherianos, I., Huvenne, H., Kanginakudru, S., Albrechtsen, M., An, C., Aymeric, J. L., Barthel, A., et al. (2010) · Journal of Insect Physiology · doi:10.1016/j.jinsphys.2010.11.006 · Cited by 869 · see citing works
Palauqui, J., Elmayan, T., Pollien, J., & Vaucheret, H. (1997) · The EMBO Journal · doi:10.1093/emboj/16.15.4738 · Cited by 780 · see citing works
RNA Interference Directs Innate Immunity Against Viruses in Adult Drosophila
Wang, X.-H., Aliyari, R., Li, W.-X., Li, H.-W., Kim, K., Carthew, R. W., Atkinson, P. W., & Ding, S. (2006) · Science · doi:10.1126/science.1125694 · Cited by 726 · see citing works
Ingested double-stranded RNAs can act as species-specific insecticides
Whyard, S., Singh, A. D., & Wong, S. (2009) · Insect Biochemistry and Molecular Biology · doi:10.1016/j.ibmb.2009.09.007 · Cited by 654 · see citing works
Systemic signalling in gene silencing
Voinnet, O., & Baulcombe, D. C. (1997) · Nature · doi:10.1038/39215 · Cited by 625 · see citing works
The endocytic pathway mediates cell entry of dsRNA to induce RNAi silencing
Saleh, M., van Rij, R. P., Hekele, A., Gillis, A. N., Foley, E., O’Farrell, P. H., & Andino, R. (2006) · Nature Cell Biology · doi:10.1038/ncb1439 · Cited by 561 · see citing works
Beyond Drosophila: RNAi In Vivo and Functional Genomics in Insects
Bellés, X. (2009) · Annual Review of Entomology · doi:10.1146/annurev-ento-112408-085301 · Cited by 447 · see citing works
Larval RNAi in Tribolium (Coleoptera) for analyzing adult development
Tomoyasu, Y., & Denell, R. E. (2004) · Development Genes and Evolution · doi:10.1007/s00427-004-0434-0 · Cited by 420 · see citing works
Tian, H., Peng, H., Yao, Q., Chen, H., Xie, Q., Tang, B., & Zhang, W. (2009) · PLoS ONE · doi:10.1371/journal.pone.0006225 · Cited by 338 · see citing works
Turner, C. T., Davy, M. W., MacDiarmid, R. M., Plummer, K. M., Birch, N. P., & Newcomb, R. D. (2006) · Insect Molecular Biology · doi:10.1111/j.1365-2583.2006.00656.x · Cited by 336 · see citing works
Zha, W., Peng, X., Chen, R., Du, B., Zhu, L., & He, G. (2011) · PLoS ONE · doi:10.1371/journal.pone.0020504 · Cited by 328 · see citing works
Amdam, G. V., Simões, Z. L. P., Guidugli, K. R., Norberg, K., & Omholt, S. W. (2003) · BMC Biotechnology · doi:10.1186/1472-6750-3-1 · Cited by 316 · see citing works
Caenorhabditis elegans SID-2 is required for environmental RNA interference
Winston, W. M., Sutherlin, M. E., Wright, A. J., Feinberg, E. H., & Hunter, C. P. (2007) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0611282104 · Cited by 309 · see citing works
Ulvila, J., Parikka, M., Kleino, A., Sormunen, R. T., Ezekowitz, R. A. B., Kocks, C., & Rämet, M. (2006) · Journal of Biological Chemistry · doi:10.1074/jbc.m513868200 · Cited by 289 · see citing works
Environmental RNA interference
Whangbo, J., & Hunter, C. P. (2008) · Trends in Genetics · doi:10.1016/j.tig.2008.03.007 · Cited by 257 · see citing works
RNA interference for the control of whiteflies (Bemisia tabaci) by oral route
Upadhyay, S. K., Chandrashekar, K., Thakur, N., Verma, P. C., Borgio, J. F., Singh, P. K., & Tuli, R. (2011) · Journal of Biosciences · doi:10.1007/s12038-011-9009-1 · Cited by 249 · see citing works
RNA interference in the termite Reticulitermes flavipes through ingestion of double-stranded RNA
Zhou, X. J., Wheeler, M. M., Oi, F. M., & Scharf, M. E. (2008) · Insect Biochemistry and Molecular Biology · doi:10.1016/j.ibmb.2008.05.005 · Cited by 230 · see citing works
Zhu, J., Liu, S., Ma, Y., Zhang, J., Qi, H.-S., Wei, Z., Yao, Q., Zhang, W., & Li, S. (2012) · PLoS ONE · doi:10.1371/journal.pone.0038572 · Cited by 171 · see citing works
Kumar, M., Gupta, G. P., & Rajam, M. V. (2009) · Journal of Insect Physiology · doi:10.1016/j.jinsphys.2008.12.005 · Cited by 169 · see citing works
Brown, S. J., Mahaffey, J. P., Lorenzen, M. D., Denell, R. E., & Mahaffey, J. W. (1999) · Evolution & Development · doi:10.1046/j.1525-142x.1999.99013.x · Cited by 163 · see citing works
Vaccari, T., Rusten, T. E., Menut, L., Nezis, I. P., Brech, A., Stenmark, H., & Bilder, D. H. (2009) · Journal of Cell Science · doi:10.1242/jcs.046391 · Cited by 153 · see citing works
Kumar, P., V, Pandit, S. S., & Baldwin, I. T. (2012) · PLoS ONE · doi:10.1371/journal.pone.0031347 · Cited by 152 · see citing works
Crop improvement using small RNAs: applications and predictive ecological risk assessments
Auer, C. A., & Frederick, R. (2009) · Trends in biotechnology · doi:10.1016/j.tibtech.2009.08.005 · Cited by 127 · see citing works
Prolonged gene knockdown in the tsetse fly Glossina by feeding double stranded RNA
Walshe, D., Lehane, S. M., Lehane, M. J., & Haines, L. R. (2008) · Insect Molecular Biology · doi:10.1111/j.1365-2583.2008.00839.x · Cited by 125 · see citing works
The Coiled-Coil Protein Shrub Controls Neuronal Morphogenesis in Drosophila
Sweeney, N. T., Brenman, J. E., Jan, Y. N., & Gao, F. (2006) · Current Biology · doi:10.1016/j.cub.2006.03.067 · Cited by 118 · see citing works
Rangasamy, M., & Siegfried, B. D. (2011) · Pest Management Science · doi:10.1002/ps.2301 · Cited by 109 · see citing works
Siegfried, B. D., Vaughn, T. T., & Spencer, T. A. (2005) · Journal of Economic Entomology · doi:10.1603/0022-0493-98.4.1320 · Cited by 108 · see citing works
siRNAs Induce Efficient RNAi Response in Bombyx mori Embryos
Yamaguchi, J., Mizoguchi, T., & Fujiwara, H. (2011) · PLoS ONE · doi:10.1371/journal.pone.0025469 · Cited by 83 · see citing works
Gene silencing in adult Aedes aegypti mosquitoes through oral delivery of double‐stranded RNA
Coy, M., Sanscrainte, N., Chalaire, K. C., Inberg, A., Maayan, I., Glick, E., Paldi, N., & Becnel, J. (2012) · Journal of Applied Entomology · doi:10.1111/j.1439-0418.2012.01713.x · Cited by 64 · see citing works
Alves, A. P., Lorenzen, M. D., Beeman, R. W., Foster, J. E., & Siegfried, B. D. (2010) · Journal of Insect Science · doi:10.1673/031.010.14122 · Cited by 58 · see citing works
Use of RNAi for Control of Insect Crop Pests
Swevers, L., & Smagghe, G. J. (2012) · Arthropod-Plant Interactions · doi:10.1007/978-94-007-3873-7_7 · Cited by 41 · see citing works
A Phagocytic Route for Uptake of Double-Stranded RNA in RNAi
Rocha, J. J. E., Korolchuk, V. I., Robinson, I. M., & O’Kane, C. J. (2011) · PLoS ONE · doi:10.1371/journal.pone.0019087 · Cited by 30 · see citing works
Suazo, A., Gore, C., & Schal, C. (2009) · Insect Molecular Biology · doi:10.1111/j.1365-2583.2009.00912.x · Cited by 25 · see citing works
Differential Physiological Roles of ESCRT Complexes in Caenorhabditis elegans
Kim, D., Sung, H., Shin, D. H., Shen, H., Ahnn, J., Lee, S., & Lee, S. (2011) · Molecules and Cells · doi:10.1007/s10059-011-1045-z · Cited by 24 · see citing works
RNAi mediated gene knockdown in sucking and chewing insect pests.
Borgio, J. F. (2010) · Journal of Biopesticides · Cited by 16 · see citing works
Flagged first, then by citations
1 of the 42 references couldn’t be matched to a known paper.
Research works printed but not linked: 2
- [23]Bucher G, Scholten J, Klinger M (2002) Parental RNAi in Tribolium (Coleoptera) Current Biology. 12: R85– R86. 11839285
- [41]Ramaseshadri P, Segers G, R F, Clinton W, Oliver I, et al.. (2012) Suppression of a component of the ESCRT-III pathway (Snf7) by oral RNAi disrupts endosomal-autophagic pathway in the western corn rootworm (Diabrotica virgifera virgifera) larvae Plos One. Under review.
Cited by
Sorted by citations
Bidirectional cross-kingdom RNAi and fungal uptake of external RNAs confer plant protection
Wang, M., Weiberg, A., Lin, F.-M., Thomma, B. P. H. J., Huang, H., & Jin, H. (2016) · Nature Plants · doi:10.1038/nplants.2016.151 · Cited by 805 · see citing works
Mechanisms, Applications, and Challenges of Insect RNA Interference
Zhu, K. Y., & Palli, S. R. (2019) · Annual Review of Entomology · doi:10.1146/annurev-ento-011019-025224 · Cited by 716 · see citing works
Surge in insect resistance to transgenic crops and prospects for sustainability
Tabashnik, B. E., & Carrière, Y. (2017) · Nature Biotechnology · doi:10.1038/nbt.3974 · Cited by 647 · see citing works
Joga, M. R., Zotti, M. J., Smagghe, G. J., & Christiaens, O. (2016) · Frontiers in Physiology · doi:10.3389/fphys.2016.00553 · Cited by 522 · see citing works
Towards the elements of successful insect RNAi
Scott, J. G., Michel, K., Bartholomay, L. C., Siegfried, B. D., Hunter, W. B., Smagghe, G. J., Zhu, K. Y., & Douglas, A. E. (2013) · Journal of Insect Physiology · doi:10.1016/j.jinsphys.2013.08.014 · Cited by 490 · see citing works
Full crop protection from an insect pest by expression of long double-stranded RNAs in plastids
Zhang, J., Khan, S. A., Hasse, C., Ruf, S., Heckel, D. G., & Bock, R. (2015) · Science · doi:10.1126/science.1261680 · Cited by 471 · see citing works
Next-generation insect-resistant plants: RNAi-mediated crop protection
Zhang, J., Khan, S. A., Heckel, D. G., & Bock, R. (2017) · Trends in Biotechnology · doi:10.1016/j.tibtech.2017.04.009 · Cited by 348 · see citing works
The next generation of insecticides: dsRNA is stable as a foliar‐applied insecticide
Miguel, K. S., & Scott, J. G. (2015) · Pest Management Science · doi:10.1002/ps.4056 · Cited by 348 · see citing works
Double-Stranded RNA Technology to Control Insect Pests: Current Status and Challenges
Christiaens, O., Whyard, S., Vélez, A. M., & Smagghe, G. J. (2020) · Frontiers in Plant Science · doi:10.3389/fpls.2020.00451 · Cited by 342 · see citing works
Genetic modification to improve disease resistance in crops
van Esse, H. P., Reuber, T., & der Does, D. V. (2019) · New Phytologist · doi:10.1111/nph.15967 · Cited by 251 · see citing works
Shukla, J. N., Kalsi, M., Sethi, A., Narva, K. E., Fishilevich, E., Singh, S., Mogilicherla, K., & Palli, S. R. (2016) · RNA Biology · doi:10.1080/15476286.2016.1191728 · Cited by 306 · see citing works
Yan, S., Ren, B., & Shen, J. (2020) · Insect Science · doi:10.1111/1744-7917.12822 · Cited by 307 · see citing works
Christiaens, O., Swevers, L., & Smagghe, G. J. (2014) · Peptides · doi:10.1016/j.peptides.2013.12.014 · Cited by 287 · see citing works
Head, G., Carroll, M. W., Evans, S. P., Rule, D. M., Willse, A., Clark, T. L., Storer, N. P., Flannagan, R. D., Samuel, L. W., & Meinke, L. J. (2017) · Pest Management Science · doi:10.1002/ps.4554 · Cited by 284 · see citing works
Yan, S., Qian, J., Cai, C., Ma, Z., Li, J., Yin, M., Ren, B., & Shen, J. (2019) · Journal of Pest Science · doi:10.1007/s10340-019-01157-x · Cited by 282 · see citing works
Management of Pest Insects and Plant Diseases by Non-Transformative RNAi
Cagliari, D., Dias, N. P., Galdeano, D. M., dos Santos, É. A., Smagghe, G. J., & Zotti, M. J. (2019) · Frontiers in Plant Science · doi:10.3389/fpls.2019.01319 · Cited by 280 · see citing works
Khajuria, C., Ivashuta, S., Wiggins, E., Flagel, L., Moar, W., Pleau, M., Miller, K., Zhang, Y., Ramaseshadri, P., Jiang, C., et al. (2018) · PLoS ONE · doi:10.1371/journal.pone.0197059 · Cited by 251 · see citing works
RNAi technology: a new platform for crop pest control
Mamta, B., & Rajam, M. V. (2017) · Physiology and Molecular Biology of Plants · doi:10.1007/s12298-017-0443-x · Cited by 246 · see citing works
Bachman, P. M., Bolognesi, R., Moar, W. J., Mueller, G. M., Paradise, M. S., Ramaseshadri, P., Tan, J., Uffman, J. P., Warren, J., Wiggins, B. E., et al. (2013) · Transgenic Research · doi:10.1007/s11248-013-9716-5 · Cited by 242 · see citing works
Genetically engineered crops help support conservation biological control
Romeis, J., Naranjo, S. E., Meissle, M., & Shelton, A. M. (2019) · Biological Control · doi:10.1016/j.biocontrol.2018.10.001 · Cited by 231 · see citing works
“virgifera, which is crucial for the transport of transmembrane proteins (Bachman et al., 2013; Bolognesi et al., 2012).”
BackgroundRNA-based technologies for insect control in plant production
Liu, S., Jaouannet, M., Dempsey, D. A., Imani, J., Coustau, C., & Kogel, K. (2019) · Biotechnology Advances · doi:10.1016/j.biotechadv.2019.107463 · Cited by 229 · see citing works
Increased RNAi Efficacy in Spodoptera exigua via the Formulation of dsRNA With Guanylated Polymers
Christiaens, O., Tardajos, M. G., Reyna, Z. M., Dash, M., Dubruel, P., & Smagghe, G. J. (2018) · Frontiers in Physiology · doi:10.3389/fphys.2018.00316 · Cited by 223 · see citing works
Cai, Q., He, B., Kogel, K., & Jin, H. (2018) · Current Opinion in Microbiology · doi:10.1016/j.mib.2018.02.003 · Cited by 223 · see citing works
Environmental Fate of Double-Stranded RNA in Agricultural Soils
Dubelman, S., Fischer, J. R., Zapata, F., Huizinga, K. M., Jiang, C., Uffman, J. P., Levine, S. L., & Carson, D. B. (2014) · PLoS ONE · doi:10.1371/journal.pone.0093155 · Cited by 219 · see citing works
RNA Interference in Insects: Protecting Beneficials and Controlling Pests
Vogel, E., Santos, D., Mingels, L., Verdonckt, T.-W., & Broeck, J. V. (2019) · Frontiers in Physiology · doi:10.3389/fphys.2018.01912 · Cited by 216 · see citing works
Parker, K. M., Barragán‐Borrero, V., van Leeuwen, D., Lever, M. A., Mateescu, B., & Sander, M. (2019) · Environmental Science & Technology · doi:10.1021/acs.est.8b05576 · Cited by 201 · see citing works
Prospects, challenges and current status of RNAi through insect feeding
Kunte, N., McGraw, E., Bell, S., Held, D. W., & Avila, L. A. (2019) · Pest Management Science · doi:10.1002/ps.5588 · Cited by 199 · see citing works
Palli, S. R. (2014) · Current Opinion in Insect Science · doi:10.1016/j.cois.2014.09.011 · Cited by 197 · see citing works
Cai, Q., He, B., Weiberg, A., Buck, A. H., & Jin, H. (2019) · PLoS Pathogens · doi:10.1371/journal.ppat.1008090 · Cited by 183 · see citing works
Yang, Z., Hu, Y., Yan, S., Zhou, H., Song, D., Yin, M., & Shen, J. (2019) · Pest Management Science · doi:10.1002/ps.5313 · Cited by 174 · see citing works
Plant and animal small RNA communications between cells and organisms
Chen, X., & Rechavi, O. (2021) · Nature Reviews Molecular Cell Biology · doi:10.1038/s41580-021-00425-y · Cited by 168 · see citing works
RNAi‐mediated plant protection against aphids
Yu, X., Liu, Z., Huang, S., Chen, Z., Sun, Y., Duan, P., Ma, Y., & Xia, L. (2016) · Pest Management Science · doi:10.1002/ps.4258 · Cited by 169 · see citing works
Progress Towards RNAi-Mediated Insect Pest Management
Baum, J. A., & Roberts, J. K. (2014) · Advances in insect physiology · doi:10.1016/b978-0-12-800197-4.00005-1 · Cited by 168 · see citing works
Off-target effects of RNAi correlate with the mismatch rate between dsRNA and non-target mRNA
Chen, J., Peng, Y., Zhang, H., Wang, K., Zhao, C., Zhu, G., Palli, S. R., & Han, Z. (2021) · RNA Biology · doi:10.1080/15476286.2020.1868680 · Cited by 165 · see citing works
RNAi for Insect Control: Current Perspective and Future Challenges
Katoch, R., Sethi, A., Thakur, N., & Murdock, L. L. (2013) · Applied Biochemistry and Biotechnology · doi:10.1007/s12010-013-0399-4 · Cited by 163 · see citing works
Improving RNAi Efficiency for Pest Control in Crop Species
Yan, S., Ren, B., Zeng, B., & Shen, J. (2020) · BioTechniques · doi:10.2144/btn-2019-0171 · Cited by 158 · see citing works
RNAi-Based Biocontrol Products: Market Status, Regulatory Aspects, and Risk Assessment
Schutter, K. D., Taning, C. N. T., Daele, L. V., Damme, E. J. M. V., Dubruel, P., & Smagghe, G. J. (2022) · Frontiers in Insect Science · doi:10.3389/finsc.2021.818037 · Cited by 158 · see citing works
Oral RNAi to control Drosophila suzukii: laboratory testing against larval and adult stages
Taning, C. N. T., Christiaens, O., Berkvens, N., Casteels, H., Maes, M., & Smagghe, G. J. (2016) · Journal of Pest Science · doi:10.1007/s10340-016-0736-9 · Cited by 157 · see citing works
The challenge of RNAi-mediated control of hemipterans
Christiaens, O., & Smagghe, G. J. (2014) · Current Opinion in Insect Science · doi:10.1016/j.cois.2014.09.012 · Cited by 151 · see citing works
Xiao, D., Gao, X., Xu, J., Xiao, L., Li, Q., Yao, J., & Zhu, K. Y. (2015) · Insect Biochemistry and Molecular Biology · doi:10.1016/j.ibmb.2015.03.009 · Cited by 139 · see citing works
RNAi as a Foliar Spray: Efficiency and Challenges to Field Applications
Hoang, L. B. T., Fletcher, S. J., Brosnan, C. A., Ghodke, A. B., Manzie, N., & Mitter, N. (2022) · International Journal of Molecular Sciences · doi:10.3390/ijms23126639 · Cited by 139 · see citing works
Knorr, E., Fishilevich, E., Tenbusch, L., Frey, M. L. F., Rangasamy, M., Billion, A., Worden, S. E., Gandra, P., Arora, K. S., Lo, W., et al. (2018) · Scientific Reports · doi:10.1038/s41598-018-20416-y · Cited by 139 · see citing works
Merzendorfer, H. (2014) · Advances in insect physiology · doi:10.1016/b978-0-12-417010-0.00001-x · Cited by 83 · see citing works
Peterson, J. A., Ode, P. J., Oliveira-Hofman, C., & Harwood, J. D. (2016) · Frontiers in Plant Science · doi:10.3389/fpls.2016.01794 · Cited by 126 · see citing works
Environmental RNAi in herbivorous insects
Ivashuta, S. I., Zhang, Y., Wiggins, B. E., Ramaseshadri, P., Segers, G. C., Johnson, S., Meyer, S. E., Kerstetter, R. A., McNulty, B. C., Bolognesi, R., et al. (2015) · RNA · doi:10.1261/rna.048116.114 · Cited by 126 · see citing works
Bachman, P. M., Huizinga, K. M., Jensen, P. D., Mueller, G., Tan, J., Uffman, J. P., & Levine, S. L. (2016) · Regulatory Toxicology and Pharmacology · doi:10.1016/j.yrtph.2016.08.001 · Cited by 123 · see citing works
Majumdar, R., Rajasekaran, K., & Cary, J. W. (2017) · Frontiers in Plant Science · doi:10.3389/fpls.2017.00200 · Cited by 120 · see citing works
RNAi-based pest control: Production, application and the fate of dsRNA
He, L., Huang, Y., & Tang, X. (2022) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2022.1080576 · Cited by 119 · see citing works
Cao, M., Gatehouse, J. A., & Fitches, E. (2018) · International Journal of Molecular Sciences · doi:10.3390/ijms19041079 · Cited by 117 · see citing works
The function of small RNAs in plant biotic stress response
Huang, J., Yang, M., & Zhang, X. (2016) · Journal of Integrative Plant Biology · doi:10.1111/jipb.12463 · Cited by 116 · see citing works
Ramaseshadri, P., Segers, G. C., Flannagan, R. S., Wiggins, E., Clinton, W. P., Ilagan, O. M., McNulty, B. C., Clark, T. L., & Bolognesi, R. (2013) · PLoS ONE · doi:10.1371/journal.pone.0054270 · Cited by 115 · see citing works
Insect pest management in the age of synthetic biology
Fernández, R. M., Petek, M., Gerasymenko, I. M., Juteršek, M., Baebler, Š., Kallam, K., Giménez, E. M., Gondolf, J., Nordmann, A., Gruden, K., et al. (2021) · Plant Biotechnology Journal · doi:10.1111/pbi.13685 · Cited by 112 · see citing works
Persistence and transgenerational effect of plant-mediated RNAi in aphids
Coleman, A. D., Wouters, R. H. M., Mugford, S. T., & Hogenhout, S. A. (2014) · Journal of Experimental Botany · doi:10.1093/jxb/eru450 · Cited by 109 · see citing works
Ma, Z., Zhang, Y., Li, M., Chao, Z., Du, X., Yan, S., & Shen, J. (2022) · Journal of Pest Science · doi:10.1007/s10340-022-01485-5 · Cited by 109 · see citing works
Höfle, L., Biedenkopf, D., Werner, B. T., Shrestha, A., Jelonek, L., & Koch, A. (2019) · RNA Biology · doi:10.1080/15476286.2019.1700033 · Cited by 106 · see citing works
Biosafety research for non-target organism risk assessment of RNAi-based GE plants
Roberts, A. F., Devos, Y., Lemgo, G., & Zhou, X. J. (2015) · Frontiers in Plant Science · doi:10.3389/fpls.2015.00958 · Cited by 104 · see citing works
Next Generation dsRNA-Based Insect Control: Success So Far and Challenges
Nitnavare, R. B., Bhattacharya, J., Singh, S., Kour, A., Hawkesford, M. J., & Arora, N. (2021) · Frontiers in Plant Science · doi:10.3389/fpls.2021.673576 · Cited by 103 · see citing works
Malik, H. J., Raza, A., Amin, I. U., Scheffler, J., Scheffler, B. E., Brown, J. K., & Mansoor, S. (2016) · Scientific Reports · doi:10.1038/srep38469 · Cited by 99 · see citing works
Christiaens, O., Sweet, J. B., Dzhambazova, T., Urru, I., Smagghe, G. J., Kostov, K. V., & Arpaia, S. (2021) · Journal of Pest Science · doi:10.1007/s10340-021-01439-3 · Cited by 95 · see citing works
Mehlhorn, S., Hunnekuhl, V. S., Geibel, S., Nauen, R., & Bucher, G. (2021) · Frontiers in Zoology · doi:10.1186/s12983-021-00444-7 · Cited by 95 · see citing works
Devos, Y., Aguilera, J., Diveki, Z., Gomes, A., Liu, Y., Paoletti, C., du Jardin, P., Herman, L., Perry, J. N., & Waigmann, E. (2014) · Transgenic Research · doi:10.1007/s11248-013-9741-4 · Cited by 95 · see citing works
Assessing the Risks of Topically Applied dsRNA-Based Products to Non-target Arthropods
Romeis, J., & Widmer, F. (2020) · Frontiers in Plant Science · doi:10.3389/fpls.2020.00679 · Cited by 95 · see citing works
Basu, S., Varsani, S., & Louis, J. (2017) · Molecular Plant-Microbe Interactions · doi:10.1094/mpmi-07-17-0183-fi · Cited by 93 · see citing works
Emerging strategies for RNA interference (RNAi) applications in insects
Nandety, R. S., Kuo, Y., Nouri, S., & Falk, B. W. (2014) · Bioengineered · doi:10.4161/21655979.2014.979701 · Cited by 92 · see citing works
In-Plant Protection against Helicoverpa armigera by Production of Long hpRNA in Chloroplasts
Bally, J., Mcintyre, G. J., Doran, R. L., Lee, K. S., Perez, A., Jung, H., Naim, F., Larrinua, I. M., Narva, K. E., & Waterhouse, P. M. (2016) · Frontiers in Plant Science · doi:10.3389/fpls.2016.01453 · Cited by 92 · see citing works
The mysteries of insect RNAi: A focus on dsRNA uptake and transport
Vélez, A. M., & Fishilevich, E. (2018) · Pesticide Biochemistry and Physiology · doi:10.1016/j.pestbp.2018.08.005 · Cited by 92 · see citing works
Aquatic fate of a double-stranded RNA in a sediment–water system following an over-water application
Fischer, J. R., Zapata, F., Dubelman, S., Mueller, G. M., Uffman, J. P., Jiang, C., Jensen, P. D., & Levine, S. L. (2016) · Environmental Toxicology and Chemistry · doi:10.1002/etc.3585 · Cited by 89 · see citing works
Ganbaatar, O., Cao, B., Zhang, Y., Bao, D., Bao, W., & Wuriyanghan, H. (2017) · BMC Biotechnology · doi:10.1186/s12896-017-0328-7 · Cited by 89 · see citing works
DsRNA-based pesticides: Considerations for efficiency and risk assessment
Dalakouras, A., Koidou, V., & Papadopoulou, K. Κ. (2024) · Chemosphere · doi:10.1016/j.chemosphere.2024.141530 · Cited by 89 · see citing works
RNAi as a management tool for the western corn rootworm, Diabrotica virgifera virgifera
Fishilevich, E., Vélez, A. M., Storer, N. P., Li, H., Bowling, A. J., Rangasamy, M., Worden, S. E., Narva, K. E., & Siegfried, B. D. (2016) · Pest Management Science · doi:10.1002/ps.4324 · Cited by 88 · see citing works
Rodrigues, T. B., & Petrick, J. S. (2020) · Frontiers in Plant Science · doi:10.3389/fpls.2020.00407 · Cited by 90 · see citing works
RNA interference tools for the western flower thrips, Frankliniella occidentalis
Badillo-Vargas, I. E., Rotenberg, D., Schneweis, B. A., & Whitfield, A. E. (2015) · Journal of Insect Physiology · doi:10.1016/j.jinsphys.2015.03.009 · Cited by 88 · see citing works
Gillet, F., Garcia, R. A., Macedo, L. L. P., Albuquerque, É. V. S., Silva, M. C. M., & Grossi‐de‐Sá, M. F. (2017) · Frontiers in Physiology · doi:10.3389/fphys.2017.00256 · Cited by 87 · see citing works
Responses of two ladybird beetle species (Coleoptera: Coccinellidae) to dietary RNAi
Haller, S., Widmer, F., Siegfried, B. D., Zhuo, X., & Romeis, J. (2019) · Pest Management Science · doi:10.1002/ps.5370 · Cited by 87 · see citing works
Gong, L., Chen, Y., Hu, Z., & Hu, M. (2013) · PLoS ONE · doi:10.1371/journal.pone.0062990 · Cited by 86 · see citing works
RNA Interference – Natural Gene-Based Technology for Highly Specific Pest Control (HiSPeC)
de Andrade, E. C., & Hunter, W. B. (2016) · InTech eBooks · doi:10.5772/61612 · Cited by 86 · see citing works
Biotic stress-associated microRNA families in plants
Šečić, E., Kogel, K., & Ladera-Carmona, M. J. (2021) · Journal of Plant Physiology · doi:10.1016/j.jplph.2021.153451 · Cited by 85 · see citing works
Miyata, K., Ramaseshadri, P., Zhang, Y., Segers, G. C., Bolognesi, R., & Tomoyasu, Y. (2014) · PLoS ONE · doi:10.1371/journal.pone.0101661 · Cited by 85 · see citing works
Gassmann, A. J. (2021) · Insects · doi:10.3390/insects12020136 · Cited by 83 · see citing works
Strategies for the production of dsRNA biocontrols as alternatives to chemical pesticides
Hough, J. A., Howard, J. D., Brown, S., Portwood, D. E., Kilby, P. M., & Dickman, M. J. (2022) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2022.980592 · Cited by 82 · see citing works
Long ds RNA but not si RNA initiates RNA i in western corn rootworm larvae and adults
Li, H., Khajuria, C., Rangasamy, M., Gandra, P., Fitter, M., Geng, C., Woosely, A., Hasler, J., Schulenberg, G., Worden, S. E., et al. (2015) · Journal of Applied Entomology · doi:10.1111/jen.12224 · Cited by 82 · see citing works
Extracellular Vesicles: Emerging Players in Plant Defense Against Pathogens
Liu, G., Kang, G., Wang, S., Huang, Y., & Cai, Q. (2021) · Frontiers in Plant Science · doi:10.3389/fpls.2021.757925 · Cited by 83 · see citing works
Galdeano, D. M., Breton, M. C., Lopes, J. R. S., Falk, B. W., & Machado, M. A. (2017) · PLoS ONE · doi:10.1371/journal.pone.0171847 · Cited by 80 · see citing works
A Transmissible RNA Pathway in Honey Bees
Maori, E., Garbian, Y., Kunik, V., Mozes-Koch, R., Malka, O., Kalev, H., Sabath, N., Sela, I., & Shafir, S. (2019) · Cell Reports · doi:10.1016/j.celrep.2019.04.073 · Cited by 80 · see citing works
He, W., Xu, W., Xu, L., Fu, K., Guo, W., Bock, R., & Zhang, J. (2020) · Journal of Experimental Botany · doi:10.1093/jxb/eraa001 · Cited by 80 · see citing works
Oberemok, V. V., Laikova, K. V., Repetskaya, A., Kenyo, I., Владимирович, Г. М., Касич, И. Н., Krasnodubets, A. M., Gal’chinsky, N. V., Фомочкина, И. И., Zaitsev, A. S., et al. (2018) · Molecules · doi:10.3390/molecules23061302 · Cited by 77 · see citing works
Efficient control of western flower thrips by plastid-mediated RNA interference
Wu, M., Dong, Y., Zhang, Q., Li, S., Chang, L., Loiacono, F. V., Ruf, S., Zhang, J., & Bock, R. (2022) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2120081119 · Cited by 77 · see citing works
Swevers, L., Broeck, J. J. M. V., & Smagghe, G. (2013) · Frontiers in Physiology · doi:10.3389/fphys.2013.00319 · Cited by 70 · see citing works
Nucleases as a barrier to gene silencing in the cotton boll weevil, Anthonomus grandis
Garcia, R. A., Macedo, L. L. P., Cabral, D., Gillet, F., Pinto, C. E. M., Faheem, M. Q., Basso, A. M. M., Silva, M. C. M., & Grossi‐de‐Sá, M. F. (2017) · PLoS ONE · doi:10.1371/journal.pone.0189600 · Cited by 77 · see citing works
Insect Resistance to Insecticides: Causes, Mechanisms, and Exploring Potential Solutions
Liang, J., Xiao, F., Ojo, J. A., Chao, W. H., Ahmad, B., Alam, A., Abbas, S., Abdelhafez, M. M., Rahman, N., Khan, K. A., et al. (2025) · Archives of Insect Biochemistry and Physiology · doi:10.1002/arch.70045 · Cited by 84 · see citing works
Chitosan nanocarriers mediated dsRNA delivery in gene silencing for Helicoverpa armigera biocontrol
Kolge, H., Kadam, K., & Ghormade, V. (2022) · Pesticide Biochemistry and Physiology · doi:10.1016/j.pestbp.2022.105292 · Cited by 75 · see citing works
Niu, X., Kassa, A., Hu, X., Robeson, J., McMahon, M., Richtman, N. M., Steimel, J. P., Kernodle, B. M., Crane, V. C., Sandahl, G. A., et al. (2017) · Scientific Reports · doi:10.1038/s41598-017-12638-3 · Cited by 74 · see citing works
Kyre, B. R., Rodrigues, T. B., & Rieske, L. K. (2019) · Scientific Reports · doi:10.1038/s41598-019-42072-6 · Cited by 73 · see citing works
Rodrigues, T. B., Rieske, L. K., Duan, J. J., Mogilicherla, K., & Palli, S. R. (2017) · Scientific Reports · doi:10.1038/s41598-017-07605-x · Cited by 73 · see citing works
RNAi for Western Corn Rootworm Management: Lessons Learned, Challenges, and Future Directions
Darlington, M., Reinders, J. D., Sethi, A., Lu, A. L., Ramaseshadri, P., Fischer, J. R., Boeckman, C. J., Petrick, J. S., Roper, J. M., Narva, K. E., et al. (2022) · Insects · doi:10.3390/insects13010057 · Cited by 72 · see citing works
Endocytic pathway mediates refractoriness of insect Bactrocera dorsalis to RNA interference
Li, X., Dong, X., Zou, C. M., & Zhang, H. (2015) · Scientific Reports · doi:10.1038/srep08700 · Cited by 71 · see citing works
Developing an in vivo toxicity assay for RNAi risk assessment in honey bees, Apis mellifera L
Vélez, A. M., Jurzenski, J., Matz, N., Zhou, X. J., Wang, H., Ellis, M. D., & Siegfried, B. D. (2015) · Chemosphere · doi:10.1016/j.chemosphere.2015.09.068 · Cited by 68 · see citing works
Plin‐amiR, a pre‐microRNA‐based technology for controlling herbivorous insect pests
Bally, J., Fishilevich, E., Doran, R. L., Lee, K. S., de Campos, S. B., German, M. A., Narva, K. E., & Waterhouse, P. M. (2020) · Plant Biotechnology Journal · doi:10.1111/pbi.13352 · Cited by 67 · see citing works
Mehlhorn, S., Geibel, S., Bucher, G., & Nauen, R. (2020) · Pesticide Biochemistry and Physiology · doi:10.1016/j.pestbp.2020.104569 · Cited by 67 · see citing works
Efficiency of RNA interference is improved by knockdown of dsRNA nucleases in tephritid fruit flies
Tayler, A., Heschuk, D., Giesbrecht, D., Park, J. Y., & Whyard, S. (2019) · Open Biology · doi:10.1098/rsob.190198 · Cited by 65 · see citing works
- Authors
- Bolognesi, R., Ramaseshadri, P., Anderson, J., Bachman, P., Clinton, W., Flannagan, R., Ilagan, O., Lawrence, C., Levine, S., Moar, W., Mueller, G., Tan, J., Uffman, J., Wiggins, E., Heck, G., & Segers, G.
- Affiliations
- Monsanto (United States) (US)
- Corresponding author
- Ramaseshadri, Parthasarathy
- Venue
- PLoS ONE
- Publisher
- Public Library of Science (PLoS)
- Published
- 2012-10-11
- Volume
- 7
- Issue
- 10
- Pages
- e47534
- ISSN
- 1932-6203 (ISSN-L)
- PMC
- PMC3469495
- OpenAlex
- W2021552468
- Cited by
- 473
- References
- 42
- Citations by year
- 2026: 24 · 2025: 49 · 2024: 29 · 2023: 31 · 2022: 52 · 2021: 44 · 2020: 43 · 2019: 50 · 2018: 39 · 2017: 28 · 2016: 33 · 2015: 24 · 2014: 14 · 2013: 13
- FWCI
- 6.52
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- PLoS ONE (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Figshare (submittedVersion, cc-by)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Insect Resistance and Genetics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Insect Pest Control Strategies
- Keywords
- western corn rootworm, RNA interference, double-stranded RNA, siRNA, oral dsRNA delivery, systemic RNAi, insect mortality, transgenic maize, insect pest control
- MeSH terms
- Analysis of Variance, Animals, Base Sequence, Coleoptera, Biological Assay, Enzyme-Linked Immunosorbent Assay, Insect Control, Larva, Lethal Dose 50, Molecular Sequence Data, RNA, Double-Stranded, Blotting, Western, Reverse Transcriptase Polymerase Chain Reaction, RNA Interference, Gastrointestinal Tract, Endosomal Sorting Complexes Required for Transport, Real-Time Polymerase Chain Reaction
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