The DOI matches its Crossref record; 59 of the 60 references it cites were found in a registry or an index.
Anxiolytic Effects in Mice of a Dual Blocker of Fatty Acid Amide Hydrolase and Transient Receptor Potential Vanilloid Type-1 Channels
Open accessAbstract
The endocannabinoid-inactivating enzyme, fatty acid amide hydrolase (FAAH), and the transient receptor potential vanilloid type-1 (TRPV1) channel are new targets for the development of anxiolytic drugs. We studied the effect on anxiety-like behavior in the elevated plus maze of a dual FAAH/TRPV1 blocker, N-arachidonoyl-serotonin (AA-5-HT). In male C57BL/6J mice, acute intraperitoneal administration of AA-5-HT (0.1-2.5 mg/kg) increased both the time spent and the number of entries in the open arm, while being inactive at the highest dose tested (5 mg/kg). AA-5-HT was more potent than selective blockers of FAAH or TRPV1 (URB597 and SB366791, respectively). In male Swiss mice, AA-5-HT had to be administered chronically to observe an anxiolytic effect at an intermediate dose (2.5 mg/kg), the highest dose (5 mg/kg) being anxiogenic, and 1 mg/kg being ineffective. In both strains, the anxiolytic effects of AA-5-HT were paralleled by elevation of brain endocannabinoid levels and were reversed by per se inactive doses of the cannabinoid receptors of type-1 (CB(1)) receptor antagonist AM251, or the TRPV1 agonist, olvanil. Immunohistochemical localization of CB(1) and TRPV1 receptors was observed in mouse prefrontal cortex, nucleus accumbens, amygdala, and hippocampus. Simultaneous 'indirect' activation of CB(1) receptors following FAAH inhibition, and antagonism at TRPV1 receptors might represent a new therapeutic strategy against anxiety.
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“the anxiolytic agent, diazepam, increases AEA in the amygdala, and bromazepam and buspirone have diminished anxiolytic effects in CB1knockout mice (Micale et al, 2009;Uriguen et al, 2004).”
From Acute stress increases circulating anandamide and other N-acylethanolamines in healthy humans (2012) · cites this paper as Micale et al, 2009
In the citing paper
“the anxiolytic agent, diazepam, increases AEA in the amygdala, and bromazepam and buspirone have diminished anxiolytic effects in CB1knockout mice (Micale et al, 2009;Uriguen et al, 2004).”
From Acute stress increases circulating anandamide and other N-acylethanolamines in healthy humans (2012) · cites this paper as Micale et al, 2009
Citations
Cites
Isolation and Structure of a Brain Constituent That Binds to the Cannabinoid Receptor
Devane, W. A., Hanuš, L., Breuer, A., Pertwee, R. G., Stevenson, L. A., Griffin, G., Gibson, D., Mandelbaum, A., Etinger, A., & Mechoulam, R. (1992) · Science · doi:10.1126/science.1470919 · Cited by 5,633
Mechoulam, R., Ben-Shabat, S., Hanus, L., Ligumsky, M., Kaminski, N. E., Schatz, A. R., Gopher, A., Almog, S., Martin, B. R., Compton, D. R., et al. (1995) · Biochemical Pharmacology · doi:10.1016/0006-2952(95)00109-d · Cited by 2,913
2-Arachidonoylgylcerol: A Possible Endogenous Cannabinoid Receptor Ligand in Brain
Sugiura, T., Kondo, S., Sukagawa, A., Nakane, S., Shinoda, A., Itoh, K., Yamashita, A., & Waku, K. (1995) · Biochemical and Biophysical Research Communications · doi:10.1006/bbrc.1995.2437 · Cited by 2,196
Vanilloid receptors on sensory nerves mediate the vasodilator action of anandamide
Zygmunt, P. M., Petersson, J., Andersson, D. A., Chuang, H.-H., Sørgård, M., Di Marzo, V., Julius, D., & Högestätt, E. D. (1999) · Nature · doi:10.1038/22761 · Cited by 2,142
The endogenous cannabinoid system controls extinction of aversive memories
Marsicano, G., Wotjak, C. T., Azad, S. C., Bisogno, T., Rammes, G., Cascio, M. G., Hermann, H., Tang, J., Hofmann, C., Zieglgänsberger, W., et al. (2002) · Nature · doi:10.1038/nature00839 · Cited by 1,728
Role of Endogenous Cannabinoids in Synaptic Signaling
Freund, T. F., Katona, I., & Piomelli, D. (2003) · Physiological Reviews · doi:10.1152/physrev.00004.2003 · Cited by 1,616
Crawley, J. N., Belknap, J. K., Collins, A. C., Crabbe, J. C., Frankel, W. N., Henderson, N. D., HITZEMANN, R. J., Maxson, S. C., Miner, L. L., Silva, A. J., et al. (1997) · Psychopharmacology · doi:10.1007/s002130050327 · Cited by 1,372
Modulation of anxiety through blockade of anandamide hydrolysis
Kathuria, S., Gaetani, S., Fegley, D., Valiño, F., Duranti, A., Tontini, A., Mor, M., Tarzia, G., Rana, G. L., Calignano, A., et al. (2003) · Nature Medicine · doi:10.1038/nm803 · Cited by 1,293
A Comprehensive Profile of Brain Enzymes that Hydrolyze the Endocannabinoid 2-Arachidonoylglycerol
Blankman, J. L., Simon, G. M., & Cravatt, B. F. (2007) · Chemistry & Biology · doi:10.1016/j.chembiol.2007.11.006 · Cited by 1,177
Huang, S. M., Bisogno, T., Trevisani, M., Al‐Hayani, A., PETROCELLIS, L. D., Fezza, F., Tognetto, M., Petros, T. J., Krey, J. F., Chu, C. J., et al. (2002) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.122196999 · Cited by 962
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Cannabinoid Receptor 1: The Neural Gatekeeper of Health and Disease
Goyal, A., Kumari, A., Verma, A., Bhatiya, S., & Yadav, H. N. (2025) · Current Neurovascular Research · doi:10.2174/0115672026403497250910124233
Boury‐Esnault, N., Marschal, C., Probst, J.-M. K., & Barnathan, G. (2002) · INFM-OAR (INFN Catania) · doi:10.5281/zenodo.155630
Stress, Anxiety, and Depression
O’Brien, K., & Bosak, C. (2025) · Springer eBooks · doi:10.1007/978-3-032-01737-6_3 · Cited by 1
Castell, L. (2020) · theses.fr (ABES) · doi:10.70675/1b15015az415cz4441zb0bdz7ce3de06f632
SZALLASI, A. (2026) · Elsevier eBooks · doi:10.1016/b978-0-443-30202-2.00007-7
O’Brien, K., & Blair, P. (2026) · Springer eBooks · doi:10.1007/978-3-032-14521-5_5
BindingDB Entry 50044981: Inhibition of FAAH, TRPV1, and COX2 by NSAID-serotonin conjugates.
BindingDB (2016) · UC San Diego · doi:10.7270/q2zs2z5t
Metadata
- Authors
- Micale, V., Cristino, L., Tamburella, A., Petrosino, S., Leggio, G. M., Drago, F., & Di Marzo, V.
- Affiliations
- University of Catania (IT)
- Institute of Applied Science and Intelligent Systems (IT)
- Istituto di Chimica Biomolecolare (IT)
- Venue
- Neuropsychopharmacology
- Published
- 2008-06-25
- Volume
- 34
- Issue
- 3
- Pages
- 593-606
- ISSN
- 0893-133X (ISSN-L)
- 1740-634X
- OpenAlex
- W2024922848
- Cited by
- 207
- References
- 60
- Citations by year
- 2026: 3 · 2025: 7 · 2024: 5 · 2023: 3 · 2022: 10 · 2021: 4 · 2020: 12 · 2019: 11 · 2018: 10 · 2017: 12 · 2016: 19 · 2015: 14 · 2014: 23 · 2013: 15 · 2012: 18
- FWCI
- 9.61
- Type
- journal article
- Language
- en
- Open access
- Licence
- other-oa
- www.springer.com/tdm (tdm)
- Locations
- Neuropsychopharmacology (publishedVersion)
- PubMed (publishedVersion)
- INFM-OAR (INFN Catania) (submittedVersion, other-oa)
- Funders
- Università di Catania
- Topics
- Health Sciences › Medicine › Pharmacology (medical) › Cannabis and Cannabinoid Research
- Life Sciences › Neuroscience › Sensory Systems › Ion Channels and Receptors
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neurotransmitter Receptor Influence on Behavior
- Keywords
- anxiolytic effects, fatty acid amide hydrolase, TRPV1, endocannabinoids, CB1 receptor, anxiety-like behavior, elevated plus maze, olvanil, URB597, SB366791, prefrontal cortex, amygdala, nucleus accumbens, hippocampus, cannabinoid receptor antagonism
- MeSH terms
- Fatty Acid Amide Hydrolases, Amidohydrolases, Anilides, Animals, Anxiety, Arachidonic Acids, Benzamides, Brain, Capsaicin, Carbamates, Cinnamates, Diazepam, Exploratory Behavior, Male, Mice, Inbred C57BL, Piperidines, Pyrazoles, Serotonin, Anti-Anxiety Agents, Receptor, Cannabinoid, CB1, TRPV Cation Channels, Mice, Cannabinoid Receptor Modulators
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