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Fusion PCR and gene targeting in Aspergillus nidulans

Szewczyk, Edyta, Nayak, Tania, Oakley, C Elizabeth, Edgerton, Heather, Xiong, Yi, Taheri-Talesh, Naimeh, Osmani, Stephen A, Oakley, Berl R·2006·Nature Protocols

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Research works printed but not linked: 14

  1. Dunne, P.W. & Oakley, B.R. Mitotic gene conversion, reciprocal recombination and gene replacement at the benA, β-tubulin locus of Aspergillus nidulans. Mol. Gen. Genet. 213, 339–345 (1988). · doi:10.1007/bf00339600
  2. Toews, M.W. et al. Establishment of mRFP1 as a fluorescent marker in Aspergillus nidulans and construction of expression vectors for high-throughput protein tagging using recombination in vitro (Gateway). Curr. Genet. 45, 383–389 (2004). · DOI
  3. Kuwayama, H. et al. PCR-mediated generation of a gene disruption construct without the use of DNA ligase and plasmid vectors. Nucleic Acids Res. 30, E2 (2002). · DOI · Paper page
  4. Yang, L. et al. Rapid production of gene replacement constructs and generation of a green fluorescent protein-tagged centromeric marker in Aspergillus nidulans. Eukaryotic Cell 3, 1359–1362 (2004). · DOI · Paper page
  5. Yu, J.-H. et al. Double-joint PCR: a PCR-based molecular tool for gene manipulations in filamentous fungi. Fungal Genet. Biol. 41, 973–981 (2004). · DOI · Paper page
  6. Nayak, T. et al. A versatile and efficient gene-targeting system for Aspergillus nidulans. Genetics 172, 1557–1566 (2006). · DOI · Paper page
  7. Zarrin, M., Leeder, A.C. & Turner, G. A rapid method for promoter exchange in Aspergillus nidulans using recombinant PCR. Fungal Genet. Biol. 42, 1–8 (2005). · doi:10.1016/j.fgb.2004.10.002
  8. Galagan, J.E. et al. Sequencing of Aspergillus nidulans and comparative analysis with A. fumigatus and A. oryzae. Nature 438, 1105–1115 (2005). · DOI · Paper page
  9. Chaveroche, M.K., Ghigo, J.M. & d'Enfert, C. A rapid method for efficient gene replacement in the filamentous fungus Aspergillus nidulans. Nucleic Acids Res. 28, E97 (2000). · DOI · Paper page
  10. Oakley, B.R. et al. Cloning, mapping and molecular analysis of the pyrG (orotidine-5′-phosphate decarboxylase) gene of Aspergillus nidulans. Gene 61, 385–399 (1987). · doi:10.1016/0378-1119(87)90201-0
  11. Osmani, A.H., Oakley, B.R. & Osmani, S.A. Identification and analysis of essential genes using the heterokaryon rescue technique in Aspergillus nidulans. Nature Protocols (in the press). DOI: 10.1038/nprot.2006.406. · DOI
  12. Vishniac, W. & Santer, M. The Thiobacilli. Bacteriol. Rev. 21, 195–213 (1957). · DOI · Paper page
  13. Innis, M.A. & Gelfand, D.H. Optimization of PCRs. In PCR Protocols (eds. Innis, M.A., Gelfand, D.H., Sninsky, J.J. & White, T.J.) 3–12 (Academic Press, San Diego, 1990).
  14. Labib, K., Diffley, J.F. & Kearsey, S.E. G1-phase and B-type cyclins exclude the DNA replication factor Mcm4 from the nucleus. Nat. Cell Biol. 1, 415–422 (1999). · DOI · Paper page

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Metadata

Authors
Szewczyk, E., Nayak, T., Oakley, C. E., Edgerton, H., Xiong, Y., Taheri-Talesh, N., Osmani, S. A., & Oakley, B. R.
Venue
Nature Protocols
Published
2006-12
Volume
1
Issue
6
Pages
3111-3120
ISSN
  • 1750-2799 (ISSN-L)
  • 1754-2189
OpenAlex
W2060081344
Cited by
766
References
0
FWCI
4.54
Type
journal article
Language
en
Open access
closed
Licence
www.springer.com/tdm (tdm)

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CoChat. (2026). Citation check: Fusion PCR and gene targeting in Aspergillus nidulans. https://app.cochat.ai/papers/10.1038/nprot.2006.405
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Szewczyk, E., Nayak, T., Oakley, C. E., Edgerton, H., Xiong, Y., Taheri-Talesh, N., Osmani, S. A., & Oakley, B. R. (2006). Fusion PCR and gene targeting in Aspergillus nidulans. Nature Protocols. https://doi.org/10.1038/nprot.2006.405

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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.

Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.