Cited by 120Fungal and yeast genetics researchCRISPR and Genetic EngineeringBacterial Genetics and Biotechnology

Validation of a Self-Excising Marker in the Human Pathogen Aspergillus fumigatus by Employing the β-Rec/ six Site-Specific Recombination System

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Hartmann, Thomas, Dümig, Michaela, Jaber, Basem M., Szewczyk, Edyta, Olbermann, Patrick, Morschhäuser, Joachim, Krappmann, Sven·2010·Applied and Environmental Microbiology

Abstract

Recyclable markers based on site-specific recombination allow repetitive gene targeting in filamentous fungi. Here we describe for the first time functionality of the bacterial recombination system employing β serine recombinase acting on six recognition sequences (β-rec/ six ) in a fungal host, the human pathogen Aspergillus fumigatus , and its use in establishing a self-excising resistance marker cassette for serial gene replacement.

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Crossref · 10.1128/aem.00882-10

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  • The low number of selection markers can be compensated for by the use of recycling systems such as the β-Rec six system.

    “This low number of selection markers can be compensated for by the use of recycling systems such as the β-Rec six system, developed by Krappmann’s group, in which the use of the recombinase allows the insertion of one six copy inside the gene deleted and the possibility to reuse the same hygromycin marker (157, 158).”

    From Aspergillus fumigatus and Aspergillosis in 2019 (2019) · cites this paper as [157]

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Authors
Hartmann, T., Dümig, M., Jaber, B. M., Szewczyk, E., Olbermann, P., Morschhäuser, J., & Krappmann, S.
Affiliations
  • Research Center for Infectious Diseases
  • University of Jordan (JO)
  • Institute for Molecular Infection Biology, Julius-Maximilians-University Würzburg, Würzburg, Germany
Venue
Applied and Environmental Microbiology
Published
2010-09-15
Volume
76
Issue
18
Pages
6313-6317
ISSN
  • 0099-2240 (ISSN-L)
  • 1098-5336
OpenAlex
W2124874710
Cited by
120
References
46
Citations by year
2026: 6 · 2025: 8 · 2024: 4 · 2023: 7 · 2022: 8 · 2021: 12 · 2020: 10 · 2019: 14 · 2018: 8 · 2017: 5 · 2016: 10 · 2015: 5 · 2014: 10 · 2013: 5 · 2012: 5
FWCI
1.69
Type
journal article
Language
en
Open access
Locations
  • Applied and Environmental Microbiology (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
Keywords
Aspergillus fumigatus, site-specific recombination, filamentous fungi
MeSH terms
Aspergillus fumigatus, DNA Nucleotidyltransferases, Genetic Engineering, Genetic Markers, Oligonucleotides, Recombination, Genetic, Gene Targeting

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