Validation of a Self-Excising Marker in the Human Pathogen Aspergillus fumigatus by Employing the β-Rec/ six Site-Specific Recombination System
Open accessAbstract
Recyclable markers based on site-specific recombination allow repetitive gene targeting in filamentous fungi. Here we describe for the first time functionality of the bacterial recombination system employing β serine recombinase acting on six recognition sequences (β-rec/ six ) in a fungal host, the human pathogen Aspergillus fumigatus , and its use in establishing a self-excising resistance marker cassette for serial gene replacement.
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The low number of selection markers can be compensated for by the use of recycling systems such as the β-Rec six system.
“This low number of selection markers can be compensated for by the use of recycling systems such as the β-Rec six system, developed by Krappmann’s group, in which the use of the recombinase allows the insertion of one six copy inside the gene deleted and the possibility to reuse the same hygromycin marker (157, 158).”
From Aspergillus fumigatus and Aspergillosis in 2019 (2019) · cites this paper as [157]
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Self-citation
- Authors
- Hartmann, T., Dümig, M., Jaber, B. M., Szewczyk, E., Olbermann, P., Morschhäuser, J., & Krappmann, S.
- Affiliations
- Research Center for Infectious Diseases
- University of Jordan (JO)
- Institute for Molecular Infection Biology, Julius-Maximilians-University Würzburg, Würzburg, Germany
- Venue
- Applied and Environmental Microbiology
- Publisher
- American Society for Microbiology
- Published
- 2010-09-15
- Volume
- 76
- Issue
- 18
- Pages
- 6313-6317
- ISSN
- 0099-2240 (ISSN-L)
- 1098-5336
- PMC
- PMC2937505
- OpenAlex
- W2124874710
- Cited by
- 120
- References
- 46
- Citations by year
- 2026: 6 · 2025: 8 · 2024: 4 · 2023: 7 · 2022: 8 · 2021: 12 · 2020: 10 · 2019: 14 · 2018: 8 · 2017: 5 · 2016: 10 · 2015: 5 · 2014: 10 · 2013: 5 · 2012: 5
- FWCI
- 1.69
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Applied and Environmental Microbiology (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Keywords
- Aspergillus fumigatus, site-specific recombination, filamentous fungi
- MeSH terms
- Aspergillus fumigatus, DNA Nucleotidyltransferases, Genetic Engineering, Genetic Markers, Oligonucleotides, Recombination, Genetic, Gene Targeting
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