Sequence-Specific Detection of Femtomolar DNA via a Chronocoulometric DNA Sensor (CDS): Effects of Nanoparticle-Mediated Amplification and Nanoscale Control of DNA Assembly at Electrodes
Abstract
We herein report a novel nanoparticle-based electrochemical DNA detection approach. This DNA sensor is based on a "sandwich" detection strategy, which involves capture probe DNA immobilized on gold electrodes and reporter probe DNA labeled with gold nanoparticles that flank the target DNA sequence. Electrochemical signals are generated by chronocoulometric interrogation of [Ru(NH(3))(6)](3+) that quantitatively binds to surface-confined capture probe DNA via electrostatic interactions. We demonstrated that the incorporation of a gold nanoparticle in this sensor design significantly enhanced the sensitivity and the selectivity. Nanoscale control of the self-assembly process of DNA probes at gold electrodes further increased the sensor performance. As a result of these two combined effects, this DNA sensor could detect as low as femtomolar (zeptomoles) DNA targets and exhibited excellent selectivity against even a single-base mismatch. In addition, this novel DNA sensor showed fairly good reproducibility, stability, and reusability.
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After cross-linking Au NPs on the electrode, surface density increased to 4.062 × 10^13 molecules cm−2, higher than conventional surface-immobilized DNA (~6 × 10^12 molecules cm−2).
“After cross-linking Au NPs on the electrode to increase the surface area, the surface density increased to 4.062 × 1013 molecules cm−2, as shown in fig. S4A, which was higher than that reported for conventional surface-immobilized DNA (~6 × 1012 molecules cm−2) (38, 43).”
From Electrochemical DNA synthesis and sequencing on a single electrode with scalability for integrated data storage (2021) · cites this paper as [38]
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- Authors
- Zhang, J., Song, S., Zhang, L., Wang, L., Wu, H., Pan, D., & Fan, C.
- Affiliations
- Chinese Academy of Sciences (CN)
- Venue
- Journal of the American Chemical Society
- Publisher
- American Chemical Society (ACS)
- Published
- 2006-06-08
- Volume
- 128
- Issue
- 26
- Pages
- 8575-8580
- ISSN
- 0002-7863 (ISSN-L)
- 1520-5126
- OpenAlex
- W2040570597
- Cited by
- 424
- References
- 32
- Citations by year
- 2026: 2 · 2025: 6 · 2024: 4 · 2023: 5 · 2022: 7 · 2021: 7 · 2020: 11 · 2019: 11 · 2018: 16 · 2017: 20 · 2016: 29 · 2015: 24 · 2014: 34 · 2013: 37 · 2012: 50
- FWCI
- 10.81
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of the American Chemical Society (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Advanced biosensing and bioanalysis techniques
- Physical Sciences › Engineering › Electrical and Electronic Engineering › Electrochemical sensors and biosensors
- Physical Sciences › Chemistry › Electrochemistry › Electrochemical Analysis and Applications
- Keywords
- DNA biosensor, chronocoulometry, gold nanoparticles, electrochemical DNA detection, sandwich immunoassay, hexaammineruthenium(III)
- MeSH terms
- DNA, Electrochemistry, Electrodes, Gold, Sensitivity and Specificity, Surface Properties, DNA Probes, Ruthenium Compounds, Nanotechnology, Nanoparticles
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