A protocol for the culture and differentiation of highly polarized human retinal pigment epithelial cells

Identification of ZO-1: a high molecular weight polypeptide associated with the tight junction (zonula occludens) in a variety of epithelia.

Stevenson, B. R., Siliciano, J. D., Mooseker, M. S., & Goodenough, D. A. (1986) · The Journal of Cell Biology · doi:10.1083/jcb.103.3.755 · Cited by 1,640 · see citing works

ARPE-19, A Human Retinal Pigment Epithelial Cell Line with Differentiated Properties

Dunn, K. C., Aotaki-Keen, A. E., Putkey, F. R., & Hjelmeland, L. M. (1996) · Experimental Eye Research · doi:10.1006/exer.1996.0020 · Cited by 1,334 · see citing works

Morphogenesis of the Polarized Epithelial Cell Phenotype

Rodriguez-Boulan, E. J., & Nelson, W. J. (1989) · Science · doi:10.1126/science.2672330 · Cited by 1,085 · see citing works

Tight junctions and the molecular basis for regulation of paracellular permeability

Anderson, J. M., & Itallie, C. M. V. (1995) · American Journal of Physiology-Gastrointestinal and Liver Physiology · doi:10.1152/ajpgi.1995.269.4.g467 · Cited by 844 · see citing works

Polarized Vascular Endothelial Growth Factor Secretion by Human Retinal Pigment Epithelium and Localization of Vascular Endothelial Growth Factor Receptors on the Inner Choriocapillaris

Blaauwgeers, H. G. T., Holtkamp, G. M., Rutten, H., Witmer, A. N., Koolwijk, P., Partanen, T. A., Alitalo, K. K., Kroon, M. E., Kijlstra, A., van Hinsbergh, V. W. M., et al. (1999) · American Journal Of Pathology · doi:10.1016/s0002-9440(10)65138-3 · Cited by 520 · see citing works

Confluent monolayers of cultured human fetal retinal pigment epithelium exhibit morphology and physiology of native tissue

Maminishkis, A., Chen, S., Jalickee, S., Banzon, T., Shi, G., Wang, F. E., Ehalt, T., Hammer, J. A., & Miller, S. S. (2006) · Investigative Opthalmology & Visual Science · doi:10.1167/iovs.05-1622 · Cited by 401 · see citing works

Novel mechanism for age‐related macular degeneration: An equilibrium shift between the angiogenesis factors VEGF and PEDF

Ohno‐Matsui, K., Morita, I., Tombran‐Tink, J., Mrazek, D., Onodera, M., Uetama, T., Hayano, M., Murota, S., & Mochizuki, M. (2001) · Journal of Cellular Physiology · doi:10.1002/jcp.10026 · Cited by 261 · see citing works

Role of the retinal pigment epithelium in macular disease.

Hogan, M. J. (1972) · PubMed · Cited by 248 · see citing works

A cell culture medium that supports the differentiation of human retinal pigment epithelium into functionally polarized monolayers.

Hu, J. G., & Bok, D. (2001) · PubMed · Cited by 165 · see citing works

Polarized Expression of Monocarboxylate Transporters in Human Retinal Pigment Epithelium and ARPE-19 Cells

Philp, N. J., Wang, D., Yoon, H., & Hjelmeland, L. M. (2003) · Investigative Ophthalmology & Visual Science · doi:10.1167/iovs.02-0287 · Cited by 163 · see citing works

The effect of metabolic inhibitors on retinal adhesion and subretinal fluid resorption.

Marmor, M. F., Abdul-Rahim, A. S., & Cohen, D. S. (1980) · PubMed · Cited by 147 · see citing works

Polarized secretion of IL-6 and IL-8 by human retinal pigment epithelial cells

Holtkamp, G. M., van Rossem, M., de Vos, A. F., Willekens, B., Peek, R., & Kijlstra, A. (1998) · Clinical & Experimental Immunology · doi:10.1046/j.1365-2249.1998.00560.x · Cited by 146 · see citing works

Polarity and the development of the outer blood-retinal barrier.

Rizzolo, L. J. (1997) · PubMed · Cited by 139 · see citing works

Ion transport mechanisms in native human retinal pigment epithelium.

Quinn, R. H., & Miller, S. S. (1992) · PubMed · Cited by 138 · see citing works

Stimulation of apical and basolateral VEGF-A and VEGF-C secretion by oxidative stress in polarized retinal pigment epithelial cells.

Kannan, R., Zhang, N., Sreekumar, P. G., Spee, C. K., Rodriguez, A., Barrón, E., & Hinton, D. R. (2006) · PubMed · Cited by 121 · see citing works

A developmentally regulated microsomal protein specific for the pigment epithelium of the vertebrate retina

Hamel, C., Tsilou, E. T., Harris, E., Pfeffer, B. A., Hooks, J. J., Detrick, B., & Redmond, T. M. (1993) · Journal of Neuroscience Research · doi:10.1002/jnr.490340406 · Cited by 117 · see citing works

Fluid transport across retinal pigment epithelium is inhibited by cyclic AMP.

Miller, S. S., Hughes, B. A., & Machen, T. E. (1982) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.79.6.2111 · Cited by 106 · see citing works

Control of Chemokine Gradients by the Retinal Pigment Epithelium

Shi, G., Maminishkis, A., Banzon, T., Jalickee, S., Li, R., Hammer, J., & Miller, S. S. (2008) · Investigative Opthalmology & Visual Science · doi:10.1167/iovs.08-1816 · Cited by 102 · see citing works

Regulation of RPE intercellular junction integrity and function by hepatocyte growth factor.

Jin, M., Barrón, E., He, S., Ryan, S. J., & Hinton, D. R. (2002) · PubMed · Cited by 88 · see citing works

Improved uranyl acetate staining for electron microscopy

Tandler, B. (1990) · Journal of Electron Microscopy Technique · doi:10.1002/jemt.1060160110 · Cited by 80 · see citing works

Characterization of Barrier Properties and Inducible VEGF Expression of Several Types of Retinal Pigment Epithelium in Medium-Term Culture

Geisen, P., McColm, J. R., King, B., & Hartnett, M. E. (2006) · Current Eye Research · doi:10.1080/02713680600837408 · Cited by 74 · see citing works

Beta adrenergic receptors on cultured human retinal pigment epithelium.

Frambach, D. A., Fain, G., Farber, D. B., & Bok, D. (1990) · PubMed · Cited by 67 · see citing works

A culture model of development reveals multiple properties of RPE tight junctions.

Ban, Y., & Rizzolo, L. J. (1997) · PubMed · Cited by 60 · see citing works

Immunocytochemical localization of Na+,K(+)-ATPase in rat retinal pigment epithelial cells.

Okami, T., Yamamoto, A., Omori, K., Takada, T., Uyama, M., & Tashiro, Y. (1990) · Journal of Histochemistry & Cytochemistry · doi:10.1177/38.9.2167328 · Cited by 56 · see citing works

Characterization of Brimonidine Transport in Retinal Pigment Epithelium

Zhang, N., Kannan, R., Okamoto, C. T., Ryan, S. J., Lee, V. H. L., & Hinton, D. R. (2005) · Investigative Ophthalmology & Visual Science · doi:10.1167/iovs.05-0189 · Cited by 46 · see citing works

Effects of RPE-cell factors secreted from permselective fibers on retinal cells in vitro

Sheedlo, H. J., Li, L., & Turner, J. E. (1992) · Brain Research · doi:10.1016/0006-8993(92)91015-7 · Cited by 42 · see citing works

Retinal pigment epithelial cells promote spatial reorganization and differentiation of retina photoreceptors

German, O. L., Buzzi, E. D., Rotstein, N. P., Rodríguez‐Boulan, E., & Politi, L. E. (2008) · Journal of Neuroscience Research · doi:10.1002/jnr.21813 · Cited by 40 · see citing works

Cell Biology of the Retinal Pigment Epithelium

Thumann, G., Hoffmann, S., & Hinton, D. R. (2006) · Elsevier eBooks · doi:10.1016/b978-0-323-02598-0.50013-6 · Cited by 39 · see citing works

N -(4-hydroxyphenyl) Retinamide Augments Laser-Induced Choroidal Neovascularization in Mice

Sreekumar, P. G., Zhou, J., Sohn, J., Spee, C. K., Ryan, S. J., Maurer, B. J., Kannan, R., & Hinton, D. R. (2008) · Investigative Ophthalmology & Visual Science · doi:10.1167/iovs.07-0667 · Cited by 27 · see citing works

Glycosaminoglycan synthesis and secretion by the retinal pigment epithelium: polarized delivery of hyaluronan from the apical surface

Senanayake, P. D., Calabro, A. R., Nishiyama, K., Hu, J. G., Bok, D., & Hollyfield, J. G. (2001) · Journal of Cell Science · doi:10.1242/jcs.114.1.199 · Cited by 26 · see citing works

Selective Increase in Basolateral Secretion of VEGF Upon BMP-4 Treatment of Highly Polarized Human RPE Cells

Sonoda, S., Sreekumar, P. G., Spee, C., Ryan, S. J., Kannan, R., & Hinton, D. R. (2008) · Investigative Ophthalmology & Visual Science · Cited by 1 · see citing works

Derivation of functional retinal pigmented epithelium from induced pluripotent stem cells

Buchholz, D. E., Hikita, S. T., Rowland, T. J., Friedrich, A. M., Hinman, C. R., Johnson, L. V., & Clegg, D. O. (2009) · Stem Cells · doi:10.1002/stem.189 · Cited by 417 · see citing works

The cell biology of the retinal pigment epithelium

Lakkaraju, A., Umapathy, A., Tan, L. X., Daniele, L., Philp, N. J., Boesze-Battaglia, K., & Williams, D. S. (2020) · Progress in Retinal and Eye Research · doi:10.1016/j.preteyeres.2020.100846 · Cited by 422 · see citing works

The oil spill in ageing Bruch membrane

Curcio, C. A., Johnson, M. F., Rudolf, M., & Huang, J.-D. (2011) · British Journal of Ophthalmology · doi:10.1136/bjophthalmol-2011-300344 · Cited by 392 · see citing works

Merging organoid and organ-on-a-chip technology to generate complex multi-layer tissue models in a human retina-on-a-chip platform

Achberger, K., Probst, C., Haderspeck, J., Bolz, S., Rogal, J., Chuchuy, J., Nikolova, M., Cora, V., Antkowiak, L., Haq, W., et al. (2019) · eLife · doi:10.7554/elife.46188 · Cited by 380 · see citing works

Biochemical adaptations of the retina and retinal pigment epithelium support a metabolic ecosystem in the vertebrate eye

Kanow, M. A., Giarmarco, M. M., Jankowski, C. S., Tsantilas, K., Engel, A. L., Du, J., Linton, J. D., Farnsworth, C. C., Sloat, S. R., Rountree, A., et al. (2017) · eLife · doi:10.7554/elife.28899 · Cited by 377 · see citing works

Soft Drusen in Age-Related Macular Degeneration: Biology and Targeting Via the Oil Spill Strategies

Curcio, C. A. (2018) · Investigative Ophthalmology & Visual Science · doi:10.1167/iovs.18-24882 · Cited by 319 · see citing works

EMT and EndMT: Emerging Roles in Age-Related Macular Degeneration

Shu, D. Y., Butcher, E. R., & Saint‐Geniez, M. (2020) · International Journal of Molecular Sciences · doi:10.3390/ijms21124271 · Cited by 293 · see citing works

Cholesterol in the retina: The best is yet to come

Pikuleva, I. A., & Curcio, C. A. (2014) · Progress in Retinal and Eye Research · doi:10.1016/j.preteyeres.2014.03.002 · Cited by 282 · see citing works

Understanding photoreceptor outer segment phagocytosis: use and utility of RPE cells in culture

Mazzoni, F., Safa, H., & Finnemann, S. C. (2014) · Experimental Eye Research · doi:10.1016/j.exer.2014.01.010 · Cited by 221 · see citing works

iPS cell modeling of Best disease: insights into the pathophysiology of an inherited macular degeneration.

Singh, R., Shen, W., Kuai, D., Martin, J., Guo, X., Smith, M. A., Perez, E. T., Phillips, M., Simonett, J. M., Wallace, K., et al. (2013) · Human Molecular Genetics · doi:10.1093/hmg/dds469 · Cited by 221 · see citing works

αB Crystallin Is Apically Secreted within Exosomes by Polarized Human Retinal Pigment Epithelium and Provides Neuroprotection to Adjacent Cells

Sreekumar, P. G., Kannan, R., Kitamura, M., Spee, C. K., Barrón, E., Ryan, S. J., & Hinton, D. R. (2010) · PLoS ONE · doi:10.1371/journal.pone.0012578 · Cited by 207 · see citing works

Stem cell based therapies for age-related macular degeneration: The promises and the challenges

Nazari, H. K., Zhang, L., Zhu, D., Chader, G. J., Falabella, P., Stefanini, F. R., Rowland, T. J., Clegg, D., Kashani, A. H., Hinton, D. R., et al. (2015) · Progress in Retinal and Eye Research · doi:10.1016/j.preteyeres.2015.06.004 · Cited by 207 · see citing works

Altered bioenergetics and enhanced resistance to oxidative stress in human retinal pigment epithelial cells from donors with age-related macular degeneration

Ferrington, D. A., Ebeling, M. C., Kapphahn, R. J., Terluk, M. R., Fisher, C. R., Polanco, J. R., Roehrich, H., Leary, M. M., Geng, Z., Dutton, J. R., et al. (2017) · Redox Biology · doi:10.1016/j.redox.2017.05.015 · Cited by 182 · see citing works

The mitochondrial-Derived peptide humanin protects RPE cells from oxidative stress, senescence, and mitochondrial dysfunction

Sreekumar, P. G., Ishikawa, K., Spee, C., Mehta, H. H., Wan, J., Yen, K., Cohen, P., Kannan, R., & Hinton, D. R. (2016) · Investigative Opthalmology & Visual Science · doi:10.1167/iovs.15-17053 · Cited by 179 · see citing works

Role of epithelial–mesenchymal transition in proliferative vitreoretinopathy

Tamiya, S., & Kaplan, H. J. (2015) · Experimental Eye Research · doi:10.1016/j.exer.2015.02.008 · Cited by 160 · see citing works

Toward the defined and xeno-free differentiation of functional human pluripotent stem cell-derived retinal pigment epithelial cells.

Vaajasaari, H., Ilmarinen, T., Juuti‐Uusitalo, K., Rajala, K. M., Onnela, N., Narkilahti, S., Suuronen, R., Hyttinen, J., Uusitalo, H. M. T., & Skottman, H. (2011) · PubMed Central · Cited by 152 · see citing works

A novel Bruch's membrane-mimetic electrospun substrate scaffold for human retinal pigment epithelium cells

Xiang, P., Wu, K., Zhu, Y., Xiang, L., Li, C., Chen, D.-L., Chen, F., Xu, G., Wang, A., Li, M., et al. (2014) · Biomaterials · doi:10.1016/j.biomaterials.2014.08.040 · Cited by 143 · see citing works

Functional Analysis of Serially Expanded Human iPS Cell-Derived RPE Cultures

Singh, R., Phillips, M. J., Kuai, D., Meyer, J., Martin, J. M., Smith, M. A., Perez, E. T., Shen, W., Wallace, K. A., Capowski, E. E., et al. (2013) · Investigative Ophthalmology & Visual Science · doi:10.1167/iovs.13-11943 · Cited by 136 · see citing works

Repressed SIRT1/PGC-1α pathway and mitochondrial disintegration in iPSC-derived RPE disease model of age-related macular degeneration

Golestaneh, N., Chu, Y., Cheng, S. K., Cao, H., Poliakov, E., & Berinstein, D. M. (2016) · Journal of Translational Medicine · doi:10.1186/s12967-016-1101-8 · Cited by 127 · see citing works

Plasma membrane protein polarity and trafficking in RPE cells: past, present and future

Lehmann, G. L., Benedicto, I., Philp, N. J., & Rodriguez-Boulan, E. (2014) · Experimental Eye Research · doi:10.1016/j.exer.2014.04.021 · Cited by 122 · see citing works

Polarized Secretion of PEDF from Human Embryonic Stem Cell–Derived RPE Promotes Retinal Progenitor Cell Survival

Zhu, D., Deng, X., Spee, C. K., Sonoda, S., Hsieh, C., Barrón, E., Pera, M. F., & Hinton, D. R. (2010) · Investigative Ophthalmology & Visual Science · doi:10.1167/iovs.10-6413 · Cited by 119 · see citing works

Implications of melanin binding in ocular drug delivery

Rimpelä, A., Reinisalo, M., Hellinen, L., Grazhdankin, E., Kidron, H., Urtti, A. O., & del Amo, E. M. (2017) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2017.12.008 · Cited by 119 · see citing works

Reductive carboxylation is a major metabolic pathway in the retinal pigment epithelium

Du, J., Yanagida, A., Knight, K., Engel, A. L., Vo, A. H., Jankowski, C. S. R., Sadı́lek, M., Tran, V., Manson, M. A., Ramakrishnan, A., et al. (2016) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1604572113 · Cited by 113 · see citing works

Isolation, culture and characterization of primary mouse RPE cells

Fernández‐Godino, R., Garland, D. L., & Pierce, E. A. (2016) · Nature Protocols · doi:10.1038/nprot.2016.065 · Cited by 106 · see citing works

Succinate Can Shuttle Reducing Power from the Hypoxic Retina to the O2-Rich Pigment Epithelium

Bisbach, C. M., Hass, D. T., Robbings, B. M., Rountree, A. M., Sadı́lek, M., Sweet, I. R., & Hurley, J. B. (2020) · Cell Reports · doi:10.1016/j.celrep.2020.107606 · Cited by 103 · see citing works

In Pursuit of Authenticity: Induced Pluripotent Stem Cell-Derived Retinal Pigment Epithelium for Clinical Applications

Miyagishima, K. J., Wan, Q., Corneo, B., Sharma, R., Lotfi, M., Boles, N. C., Hua, F., Maminishkis, A., Zhang, C., Blenkinsop, T. A., et al. (2016) · Stem Cells Translational Medicine · doi:10.5966/sctm.2016-0037 · Cited by 102 · see citing works

Glutathione Metabolism and the Novel Role of Mitochondrial GSH in Retinal Degeneration

Sreekumar, P. G., Ferrington, D. A., & Kannan, R. (2021) · Antioxidants · doi:10.3390/antiox10050661 · Cited by 103 · see citing works

Induction of differentiation by pyruvate and DMEM in the human retinal pigment epithelium cell line ARPE-19

Ahmado, A., Carr, A.-J., Vugler, A. A., Semo, M., Gias, C., Lawrence, J. M., Chen, L. L., Chen, F. K., Turowski, P., da Cruz, L., et al. (2011) · Investigative Opthalmology & Visual Science · doi:10.1167/iovs.10-6374 · Cited by 100 · see citing works

Differential Metabolic Reprogramming by Zika Virus Promotes Cell Death in Human versus Mosquito Cells

Thaker, S. K., Chapa, T. J., Garcia, G., Gong, D., Schmid, E. W., Arumugaswami, V., Sun, R., & Christofk, H. R. (2019) · Cell Metabolism · doi:10.1016/j.cmet.2019.01.024 · Cited by 97 · see citing works

The culture and maintenance of functional retinal pigment epithelial monolayers from adult human eye

Blenkinsop, T. A., Salero, E., Stern, J. H., & Temple, S. (2012) · Methods in molecular biology · doi:10.1007/978-1-62703-125-7_4 · Cited by 96 · see citing works

A Role for βA3/A1-Crystallin in Type 2 EMT of RPE Cells Occurring in Dry Age-Related Macular Degeneration

Ghosh, S., Shang, P., Terasaki, H., Stepicheva, N. A., Hose, S., Yazdankhah, M., Weiss, J., Sakamoto, T., Bhutto, I. A., Xia, S., et al. (2018) · Investigative Ophthalmology & Visual Science · doi:10.1167/iovs.18-24132 · Cited by 96 · see citing works

Human retinal pigment epithelial cells prefer proline as a nutrient and transport metabolic intermediates to the retinal side

Chao, J. R., Knight, K., Engel, A. L., Jankowski, C. S. R., Wang, Y., Manson, M. A., Gu, H., Djukovic, D., Raftery, D., Hurley, J. B., et al. (2017) · Journal of Biological Chemistry · doi:10.1074/jbc.m117.788422 · Cited by 93 · see citing works

Cell culture models to study retinal pigment epithelium-related pathogenesis in age-related macular degeneration

Bharti, K., den Hollander, A. I., Lakkaraju, A., Sinha, D., Williams, D. S., Finnemann, S. C., Rickman, C. B., Malek, G., & D'Amore, P. A. (2022) · Experimental Eye Research · doi:10.1016/j.exer.2022.109170 · Cited by 92 · see citing works

Attainment of polarity promotes growth factor secretion by retinal pigment epithelial cells: Relevance to age-related macular degeneration

Sonoda, S., Sreekumar, P. G., Kase, S., Spee, C. K., Ryan, S. J., Kannan, R., & Hinton, D. R. (2009) · Aging · doi:10.18632/aging.100111 · Cited by 92 · see citing works

Conditional knockdown of DNA methyltransferase 1 reveals a key role of retinal pigment epithelium integrity in photoreceptor outer segment morphogenesis

Nasonkin, I. O., Merbs, S. L., Lazo, K., Oliver, V. F., Brooks, M., Patel, K., Enke, R. A., Nellissery, J. K., Jamrich, M., Le, Y. Z., et al. (2013) · Development · doi:10.1242/dev.086603 · Cited by 90 · see citing works

Extracellular vesicles released by human retinal pigment epithelium mediate increased polarised secretion of drusen proteins in response to AMD stressors

Flores‐Bellver, M., Mighty, J., Aparicio‐Domingo, S., Li, K. V., Shi, C., Zhou, J., Cobb, H., McGrath, P. S., Michelis, G., Lenhart, P., et al. (2021) · Journal of Extracellular Vesicles · doi:10.1002/jev2.12165 · Cited by 87 · see citing works

The cultivation of human retinal pigment epithelial cells on Bombyx mori silk fibroin

Shadforth, A. M. A., George, K. A., Kwan, A., Chirilă, T. V., & Harkin, D. G. (2012) · Biomaterials · doi:10.1016/j.biomaterials.2012.02.040 · Cited by 90 · see citing works

Proline mediates metabolic communication between retinal pigment epithelial cells and the retina

Yam, M., Engel, A. L., Wang, Y., Zhu, S., Hauer, A., Zhang, R., Lohner, D., Huang, J., Dinterman, M., Zhao, C., et al. (2019) · Journal of Biological Chemistry · doi:10.1074/jbc.ra119.007983 · Cited by 89 · see citing works

Human Adult Retinal Pigment Epithelial Stem Cell–Derived RPE Monolayers Exhibit Key Physiological Characteristics of Native Tissue

Blenkinsop, T. A., Saini, J. S., Maminishkis, A., Bharti, K., Wan, Q., Banzon, T. M., Lotfi, M., Davis, J. A., Singh, D., Rizzolo, L. J., et al. (2015) · Investigative Ophthalmology & Visual Science · doi:10.1167/iovs.14-16246 · Cited by 87 · see citing works

Gene Therapy in Patient-specific Stem Cell Lines and a Preclinical Model of Retinitis Pigmentosa With Membrane Frizzled-related Protein Defects

Li, Y., Wu, W.-H., Hsu, C.-W., Nguyen, H. V., Tsai, Y., Chan, L., Nagasaki, T., Maumenee, I. H., Yannuzzi, L. A., Hoang, Q. V., et al. (2014) · Molecular Therapy · doi:10.1038/mt.2014.100 · Cited by 84 · see citing works

A local complement response by RPE causes early-stage macular degeneration

Fernandez-Godino, R., Garland, D. L., & Pierce, E. A. (2015) · Human Molecular Genetics · doi:10.1093/hmg/ddv287 · Cited by 73 · see citing works

Directional protein secretion by the retinal pigment epithelium: roles in retinal health and the development of age‐related macular degeneration

Kay, P., Yang, Y. C., & Paraoan, L. (2013) · Journal of Cellular and Molecular Medicine · doi:10.1111/jcmm.12070 · Cited by 81 · see citing works

Methods for culturing retinal pigment epithelial cells: a review of current protocols and future recommendations

Fronk, A., & Vargis, E. (2016) · Journal of Tissue Engineering · doi:10.1177/2041731416650838 · Cited by 81 · see citing works

Differentiation Therapy: Targeting Human Renal Cancer Stem Cells with Interleukin 15

Azzi, S., Bruno, S., Giron‐Michel, J., Clay, D., Devocelle, A., Croce, M., Ferrini, S., Chouaı̈b, S., Vázquez, A., Charpentier, B., et al. (2011) · JNCI Journal of the National Cancer Institute · doi:10.1093/jnci/djr451 · Cited by 79 · see citing works

Resveratrol inhibits epithelial-mesenchymal transition of retinal pigment epithelium and development of proliferative vitreoretinopathy

Ishikawa, K., He, S., Terasaki, H., Nazari, H. K., Zhang, H., Spee, C. K., Kannan, R., & Hinton, D. R. (2015) · Scientific Reports · doi:10.1038/srep16386 · Cited by 79 · see citing works

Malondialdehyde induces autophagy dysfunction and VEGF secretion in the retinal pigment epithelium in age-related macular degeneration

Ye, F., Kaneko, H., Hayashi, Y., Takayama, K., Hwang, S.-J., Nishizawa, Y., Kimoto, R., Nagasaka, Y., Tsunekawa, T., Matsuura, T., et al. (2016) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2016.02.027 · Cited by 78 · see citing works

Protection of Retina by αB Crystallin in Sodium Iodate Induced Retinal Degeneration

Zhou, P., Kannan, R., Spee, C. K., Sreekumar, P. G., Dou, G., & Hinton, D. R. (2014) · PLoS ONE · doi:10.1371/journal.pone.0098275 · Cited by 77 · see citing works

Cell Models to Study Regulation of Cell Transformation in Pathologies of Retinal Pigment Epithelium

Kuznetsova, A. V., Kurinov, A. M., & Aleksandrova, M. A. (2014) · Journal of Ophthalmology · doi:10.1155/2014/801787 · Cited by 77 · see citing works

Protective Mechanisms of the Mitochondrial‐Derived Peptide Humanin in Oxidative and Endoplasmic Reticulum Stress in RPE Cells

Minasyan, L., Sreekumar, P. G., Hinton, D. R., & Kannan, R. (2017) · Oxidative Medicine and Cellular Longevity · doi:10.1155/2017/1675230 · Cited by 77 · see citing works

A New Efficient Protocol for Directed Differentiation of Retinal Pigmented Epithelial Cells from Normal and Retinal Disease Induced Pluripotent Stem Cells

Zahabi, A., Shahbazi, E., Ahmadieh, H., Hassani, S., Totonchi, M., Taei, A., Masoudi, N., Ebrahimi, M., Aghdami, N., Seifinejad, A., et al. (2011) · Stem Cells and Development · doi:10.1089/scd.2011.0599 · Cited by 74 · see citing works

NF-κB Is the Transcription Factor for FGF-2 That Causes Endothelial Mesenchymal Transformation in Cornea

Lee, J. G., & Kay, E. P. (2012) · Investigative Ophthalmology & Visual Science · doi:10.1167/iovs.11-9102 · Cited by 74 · see citing works

A detailed three-step protocol for live imaging of intracellular traffic in polarized primary porcine RPE monolayers

Toops, K. A., Tan, L. X., & Lakkaraju, A. (2014) · Experimental Eye Research · doi:10.1016/j.exer.2014.05.003 · Cited by 73 · see citing works

Cultured primary human fetal retinal pigment epithelium (hfRPE) as a model for evaluating RPE metabolism

Adijanto, J., & Philp, N. J. (2014) · Experimental Eye Research · doi:10.1016/j.exer.2014.01.015 · Cited by 73 · see citing works

Using human induced pluripotent stem cells to treat retinal disease

Borooah, S., Phillips, M. J., Bilican, B., Wright, A. F., Wilmut, I., Chandran, S., Gamm, D. M., & Dhillon, B. (2013) · Progress in Retinal and Eye Research · doi:10.1016/j.preteyeres.2013.09.002 · Cited by 71 · see citing works

Changes in extracellular matrix cause RPE cells to make basal deposits and activate the alternative complement pathway

Fernández‐Godino, R., Bujakowska, K. M., & Pierce, E. A. (2017) · Human Molecular Genetics · doi:10.1093/hmg/ddx392 · Cited by 71 · see citing works

Microglia Contribution to the Regulation of the Retinal and Choroidal Vasculature in Age-Related Macular Degeneration

Alves, C. H., Fernandes, R., Santiago, A. R., & Ambrósio, A. F. (2020) · Cells · doi:10.3390/cells9051217 · Cited by 71 · see citing works

Cellular models and therapies for age-related macular degeneration

Forest, D. L., Johnson, L. V., & Clegg, D. (2015) · Disease Models & Mechanisms · doi:10.1242/dmm.017236 · Cited by 69 · see citing works

TNF-α disrupts morphologic and functional barrier properties of polarized retinal pigment epithelium

Shirasawa, M., Sonoda, S., Terasaki, H., Arimura, N., Otsuka, H., Yamashita, T., Uchino, E., Hisatomi, T., Ishibashi, T., & Sakamoto, T. (2013) · Experimental Eye Research · doi:10.1016/j.exer.2013.02.012 · Cited by 68 · see citing works

Differentiation of RPE cells from integration-free iPS cells and their cell biological characterization

Hazim, R. A., Karumbayaram, S., Jiang, M., Dimashkie, A., Lopes, V. S., Li, D., Burgess, B. L., Vijayaraj, P., Alva-Ornelas, J. A., Zack, J. A., et al. (2017) · Stem Cell Research & Therapy · doi:10.1186/s13287-017-0652-9 · Cited by 66 · see citing works

Toll-like Receptor 3 Is Required for Development of Retinopathy Caused by Impaired All-trans-retinal Clearance in Mice

Shiose, S., Chen, Y., Okano, K., Roy, S., Kohno, H., Tang, J. C. O., Pearlman, E. M., Maeda, T., Palczewski, K., & Maeda, A. (2011) · Journal of Biological Chemistry · doi:10.1074/jbc.m111.228551 · Cited by 64 · see citing works

Erythropoietin protects outer blood‐retinal barrier in experimental diabetic retinopathy by up‐regulating ZO‐1 and occludin

Zhang, C., Xie, H., Yang, Q., Yang, Y., Li, W., Tian, H., Lü, L., Wang, F., Xu, J., Gao, F., et al. (2019) · Clinical and Experimental Ophthalmology · doi:10.1111/ceo.13619 · Cited by 65 · see citing works

Polarized Human Embryonic Stem Cell-Derived Retinal Pigment Epithelial Cell Monolayers Have Higher Resistance to Oxidative Stress-Induced Cell Death Than Nonpolarized Cultures

Hsiung, J., Zhu, D., & Hinton, D. R. (2014) · Stem Cells Translational Medicine · doi:10.5966/sctm.2014-0205 · Cited by 61 · see citing works

αB-Crystallin Regulates Subretinal Fibrosis by Modulation of Epithelial-Mesenchymal Transition

Ishikawa, K., Sreekumar, P. G., Spee, C. K., Nazari, H. K., Zhu, D., Kannan, R., & Hinton, D. R. (2016) · American Journal Of Pathology · doi:10.1016/j.ajpath.2015.11.014 · Cited by 61 · see citing works

Abnormal mTORC1 signaling leads to retinal pigment epithelium degeneration

Huang, J., Gu, S., Chen, M., Zhang, S., Jiang, Z., Chen, X., Jiang, C., Liu, G., Radu, R. A., Sun, X., et al. (2019) · Theranostics · doi:10.7150/thno.26281 · Cited by 60 · see citing works

Polarity and epithelial-mesenchymal transition of retinal pigment epithelial cells in proliferative vitreoretinopathy

Zou, H., Shan, C., Ma, L., Liu, J., Yang, N., & Zhao, J. (2020) · PeerJ · doi:10.7717/peerj.10136 · Cited by 59 · see citing works

Over-expression of BMP4 inhibits experimental choroidal neovascularization by modulating VEGF and MMP-9

Xu, J., Zhu, D., Sonoda, S., He, S., Spee, C. K., Ryan, S. J., & Hinton, D. R. (2012) · Angiogenesis · doi:10.1007/s10456-012-9254-4 · Cited by 55 · see citing works

Patient-specific mutations impair BESTROPHIN1’s essential role in mediating Ca2+-dependent Cl- currents in human RPE

Li, Y., Zhang, Y., Xu, Y. H., Kittredge, A., Ward, N., Chen, S., Tsang, S. H., & Yang, T. (2017) · eLife · doi:10.7554/elife.29914 · Cited by 54 · see citing works

Mechanism of RPE Cell Death in α-Crystallin Deficient Mice: A Novel and Critical Role for MRP1-Mediated GSH Efflux

Sreekumar, P. G., Spee, C. K., Ryan, S. J., Cole, S. P. C., Kannan, R., & Hinton, D. R. (2012) · PLoS ONE · doi:10.1371/journal.pone.0033420 · Cited by 54 · see citing works

Retinal pigment epithelium (RPE) cytoskeleton in vivo and in vitro

Bonilha, V. L. (2013) · Experimental Eye Research · doi:10.1016/j.exer.2013.09.015 · Cited by 52 · see citing works

Protein polymer nanoparticles engineered as chaperones protect against apoptosis in human retinal pigment epithelial cells

Wang, W., Sreekumar, P. G., Valluripalli, V., Shi, P., Wang, J., Lin, Y., Cui, H., Kannan, R., Hinton, D. R., & MacKay, J. A. (2014) · Journal of Controlled Release · doi:10.1016/j.jconrel.2014.04.028 · Cited by 52 · see citing works

Intra-vitreal αB crystallin fused to elastin-like polypeptide provides neuroprotection in a mouse model of age-related macular degeneration

Sreekumar, P. G., Li, Z., Wang, W., Spee, C. K., Hinton, D. R., Kannan, R., & MacKay, J. A. (2018) · Journal of Controlled Release · doi:10.1016/j.jconrel.2018.05.014 · Cited by 52 · see citing works

Inhibition of PVR with a Tyrosine Kinase Inhibitor, Dasatinib, in the Swine

Umazume, K., Liu, L., Scott, P. A., de Castro, J. P. F., McDonald, K., Kaplan, H. J., & Tamiya, S. (2013) · Investigative Ophthalmology & Visual Science · doi:10.1167/iovs.12-10418 · Cited by 51 · see citing works

Rapid, Directed Differentiation of Retinal Pigment Epithelial Cells from Human Embryonic or Induced Pluripotent Stem Cells

Foltz, L. P., & Clegg, D. (2017) · Journal of Visualized Experiments · doi:10.3791/56274 · Cited by 51 · see citing works

TNF-α Decreases VEGF Secretion in Highly Polarized RPE Cells but Increases It in Non-Polarized RPE Cells Related to Crosstalk between JNK and NF-κB Pathways

Terasaki, H., Kase, S., Shirasawa, M., Otsuka, H., Hisatomi, T., Sonoda, S., Ishida, S., Ishibashi, T., & Sakamoto, T. (2013) · PLoS ONE · doi:10.1371/journal.pone.0069994 · Cited by 50 · see citing works

Acetylated bacterial cellulose coated with urinary bladder matrix as a substrate for retinal pigment epithelium

Gonçalves, S., Rodrigues, I. P., Padrão, J., Silva, J. P., Sencadas, V., Lanceros-Méndez, S., Girão, H., Gama, M., Dourado, F. O. Q., & Rodrigues, L. R. (2015) · Colloids and Surfaces B Biointerfaces · doi:10.1016/j.colsurfb.2015.11.051 · Cited by 50 · see citing works

LC–MS/MS Based Quantitation of ABC and SLC Transporter Proteins in Plasma Membranes of Cultured Primary Human Retinal Pigment Epithelium Cells and Immortalized ARPE19 Cell Line

Pelkonen, L., Sato, K., Reinisalo, M., Kidron, H., Tachikawa, M., Watanabe, M., Uchida, Y., Urtti, A. O., & Terasaki, T. (2017) · Molecular Pharmaceutics · doi:10.1021/acs.molpharmaceut.6b00782 · Cited by 50 · see citing works

Replication ofMycobacterium Tuberculosisin Retinal Pigment Epithelium

Nazari, H. K., Karakousis, P. C., & Rao, N. A. (2014) · JAMA Ophthalmology · doi:10.1001/jamaophthalmol.2014.270 · Cited by 49 · see citing works

Fenofibric acid prevents retinal pigment epithelium disruption induced by interleukin-1β by suppressing AMP-activated protein kinase (AMPK) activation

Villarroel, M., García‐Ramírez, M., Corraliza, L., Hernández, C., & Simó, R. (2011) · Diabetologia · doi:10.1007/s00125-011-2089-5 · Cited by 47 · see citing works

Docosahexaenoic acid aggravates photooxidative damage in retinal pigment epithelial cells via lipid peroxidation

Liu, Y., Zhang, D., Yong-pei, W., & Ji, B. (2014) · Journal of Photochemistry and Photobiology B Biology · doi:10.1016/j.jphotobiol.2014.07.016 · Cited by 47 · see citing works

A TGF-β receptor 1 inhibitor for prevention of proliferative vitreoretinopathy

Nassar, K., Grisanti, S., Tura, A., Lüke, J., Lüke, M., Soliman, M. M., & Grisanti, S. (2014) · Experimental Eye Research · doi:10.1016/j.exer.2014.04.006 · Cited by 45 · see citing works

Decorin Prevents Retinal Pigment Epithelial Barrier Breakdown Under Diabetic Conditions by Suppressing p38 MAPK Activation

Wang, S., Du, S., Wu, Q., Hu, J., & Li, T. (2015) · Investigative Opthalmology & Visual Science · doi:10.1167/iovs.14-15874 · Cited by 44 · see citing works

A Bruch's membrane substitute fabricated from silk fibroin supports the function of retinal pigment epithelial cells in vitro

Shadforth, A. M. A., Suzuki, S., Theodoropoulos, C., Richardson, N. A., Chirilă, T. V., & Harkin, D. G. (2015) · Journal of Tissue Engineering and Regenerative Medicine · doi:10.1002/term.2089 · Cited by 44 · see citing works

TGF-β2 secretion from RPE decreases with polarization and becomes apically oriented

Hirsch, L., Nazari, H. K., Sreekumar, P. G., Kannan, R., Dustin, L., Zhu, D., Barrón, E., & Hinton, D. R. (2014) · Cytokine · doi:10.1016/j.cyto.2014.11.014 · Cited by 43 · see citing works

N-Terminomics identifies HtrA1 cleavage of thrombospondin-1 with generation of a proangiogenic fragment in the polarized retinal pigment epithelial cell model of age-related macular degeneration

Chen, C.-Y., Melo, E., Jakob, P., Friedlein, A., Elsässer, B., Goettig, P., Küppers, V., Delobel, F., Stucki, C., Dunkley, T. P. J., et al. (2018) · Matrix Biology · doi:10.1016/j.matbio.2018.03.013 · Cited by 43 · see citing works

Expression and regulation of enzymes in the ceramide metabolic pathway in human retinal pigment epithelial cells and their relevance to retinal degeneration

Zhu, D., Sreekumar, P. G., Hinton, D. R., & Kannan, R. (2009) · Vision Research · doi:10.1016/j.visres.2009.09.002 · Cited by 41 · see citing works

Proliferative Vitreoretinopathy in the Swine–A New Model

Umazume, K., Barak, Y., McDonald, K., Liu, L., Kaplan, H. J., & Tamiya, S. (2012) · Investigative Ophthalmology & Visual Science · doi:10.1167/iovs.12-9768 · Cited by 42 · see citing works

A Multi-Omics Approach Identifies Key Regulatory Pathways Induced by Long-Term Zinc Supplementation in Human Primary Retinal Pigment Epithelium

Emri, E., Körtvely, E., Dammeier, S., Klose, F., Simpson, D. A. C., den Hollander, A. I., Ueffing, M., & Lengyel, I. (2020) · Nutrients · doi:10.3390/nu12103051 · Cited by 41 · see citing works

Mechanisms of RPE senescence and potential role of αB crystallin peptide as a senolytic agent in experimental AMD

Sreekumar, P. G., Reddy, S. T., Hinton, D. R., & Kannan, R. (2022) · Experimental Eye Research · doi:10.1016/j.exer.2021.108918 · Cited by 41 · see citing works

Sonoporation Enhances Chemotherapeutic Efficacy in Retinoblastoma Cells In Vitro

Lee, N. G., Berry, J. L., Lee, T. C., Wang, A. T., Honowitz, S., Murphree, A. L., Varshney, N., Hinton, D. R., & Fawzi, A. A. (2011) · Investigative Ophthalmology & Visual Science · doi:10.1167/iovs.10-6501 · Cited by 40 · see citing works

Recent advances and future prospects: Current status and challenges of the intraocular injection of drugs for vitreoretinal diseases

Hashida, N., & Nishida, K. (2023) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2023.114870 · Cited by 37 · see citing works

Advances in retinal pigment epithelial cell transplantation for retinal degenerative diseases

Liu, H., Huang, S. S., Gopal, L., Kai, D., Su, X., & Liu, Z. (2024) · Stem Cell Research & Therapy · doi:10.1186/s13287-024-04007-5 · Cited by 38 · see citing works

Soluble EphB4 Inhibition of PDGF-Induced RPE Migration In Vitro

He, S., Kumar, S. R., Zhou, P., Krasnoperov, V. G., Ryan, S. J., Gill, P. S., & Hinton, D. R. (2010) · Investigative Ophthalmology & Visual Science · doi:10.1167/iovs.09-3475 · Cited by 38 · see citing works

Role of Retinal Pigment Epithelial Cell β-Catenin Signaling in Experimental Proliferative Vitreoretinopathy

Umazume, K., Tsukahara, R., Liu, L., de Castro, J. P. F., McDonald, K., Kaplan, H. J., & Tamiya, S. (2014) · American Journal Of Pathology · doi:10.1016/j.ajpath.2014.01.022 · Cited by 37 · see citing works

Advances in Retinal Tissue Engineering

Trese, M. G. J., Regatieri, C. V., & Young, M. J. (2012) · Materials · doi:10.3390/ma5010108 · Cited by 37 · see citing works

Lipid Nanoparticle-Mediated Delivery of mRNA Into the Mouse and Human Retina and Other Ocular Tissues

Chambers, C., Soo, G. L., Engel, A. L., Glass, I. A., Frassetto, A., Martini, P. G. V., & Cherry, T. J. (2024) · Translational Vision Science & Technology · doi:10.1167/tvst.13.7.7 · Cited by 36 · see citing works

Human embryonic stem cell applications for retinal degenerations

Reynolds, J. C., & Lamba, D. A. (2013) · Experimental Eye Research · doi:10.1016/j.exer.2013.07.010 · Cited by 36 · see citing works

Recent advances in drug and nutrient transport across the blood-retinal barrier

Kubo, Y., Akanuma, S., & Hosoya, K. (2018) · Expert Opinion on Drug Metabolism & Toxicology · doi:10.1080/17425255.2018.1472764 · Cited by 36 · see citing works

In Vitro Epiretinal Membrane Model and Antibody Permeability: Relationship With Anti-VEGF Resistance in Diabetic Macular Edema

Namba, R., Kaneko, H., Suzumura, A., Shimizu, H., Kataoka, K., Takayama, K., Yamada, K., Funahashi, Y., Ito, S., Nonobe, N., et al. (2019) · Investigative Ophthalmology & Visual Science · doi:10.1167/iovs.19-26788 · Cited by 36 · see citing works

Reproducible production and image-based quality evaluation of retinal pigment epithelium sheets from human induced pluripotent stem cells

Ye, K., Takemoto, Y., Ito, A., Onda, M., Morimoto, N., Mandai, M., Takahashi, M., Kato, R., & Osakada, F. (2020) · Scientific Reports · doi:10.1038/s41598-020-70979-y · Cited by 36 · see citing works

Modulation of MCT3 Expression during Wound Healing of the Retinal Pigment Epithelium

Gallagher‐Colombo, S. M., Maminishkis, A., Tate, S., Grunwald, G. B., & Philp, N. J. (2010) · Investigative Ophthalmology & Visual Science · doi:10.1167/iovs.09-5028 · Cited by 36 · see citing works

A cell culture medium that supports the differentiation of human retinal pigment epithelium into functionally polarized monolayers.

Hu, J. G., & Bok, D. (2001) · PubMed · Cited by 165 · see citing works

Primary Culture of Porcine Retinal Pigment Epithelial Cells

Wen, F., Wang, Y., He, D., Liao, C., Ouyang, W., Liu, Z., Li, W. S., & Liao, Y. (2022) · Journal of Visualized Experiments · doi:10.3791/64244

A detailed three-step protocol for live imaging of intracellular traffic in polarized primary porcine RPE monolayers

Toops, K. A., Tan, L. X., & Lakkaraju, A. (2014) · Experimental Eye Research · doi:10.1016/j.exer.2014.05.003 · Cited by 73 · see citing works

Patch culturing and transfer of human fetal retinal epithelium

Gouras, P., Cao, H., 盛耀华, Tanabe, T., Efremova, Y., & Kjeldbye, H. M. (1994) · Graefe s Archive for Clinical and Experimental Ophthalmology · doi:10.1007/bf00193120 · Cited by 29 · see citing works

Isolation, culture and characterization of primary mouse RPE cells

Fernández‐Godino, R., Garland, D. L., & Pierce, E. A. (2016) · Nature Protocols · doi:10.1038/nprot.2016.065 · Cited by 106 · see citing works