The DOI matches its Crossref record; 15 of the 15 references it cites were found in a registry or an index.
Generating human intestinal tissue from pluripotent stem cells in vitro
Open accessAbstract
Here we describe a protocol for generating 3D human intestinal tissues (called organoids) in vitro from human pluripotent stem cells (hPSCs). To generate intestinal organoids, pluripotent stem cells are first differentiated into FOXA2+SOX17+ endoderm by treating the cells with activin A for 3 d. After endoderm induction, the pluripotent stem cells are patterned into CDX2+ mid- and hindgut tissue using FGF4 and WNT3a. During this patterning step, 3D mid- or hindgut spheroids bud from the monolayer epithelium attached to the tissue culture dish. The 3D spheroids are further cultured in Matrigel along with prointestinal growth factors, and they proliferate and expand over 1–3 months to give rise to intestinal tissue, complete with intestinal mesenchyme and epithelium comprising all of the major intestinal cell types. To date, this is the only method for efficiently directing the differentiation of hPSCs into 3D human intestinal tissue in vitro.
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Single Lgr5 stem cells build crypt-villus structures in vitro without a mesenchymal niche
Sato, T., Vries, R. G., Snippert, H. J., van de Wetering, M., Barker, N., Stange, D. E., van Es, J. H., Abo, A., Kujala, P., Peters, P. J., et al. (2009) · Nature · doi:10.1038/nature07935 · Cited by 7,204
Directed differentiation of human pluripotent stem cells into intestinal tissue in vitro
Spence, J. R., Mayhew, C. N., Rankin, S. A., Kuhar, M. F., Vallance, J. E., Tolle, K., Hoskins, E. E., Kalinichenko, V. V., Wells, S. I., Zorn, A. M., et al. (2011) · Nature · doi:10.1038/nature09691 · Cited by 1,922
Self-citationStem Cells, Self-Renewal, and Differentiation in the Intestinal Epithelium
van der Flier, L. G., & Clevers, H. (2009) · Annual Review of Physiology · doi:10.1146/annurev.physiol.010908.163145 · Cited by 1,871
McLin, V. A., Rankin, S. A., & Zorn, A. M. (2007) · Development · doi:10.1242/dev.001230 · Cited by 368
Early mouse endoderm is patterned by soluble factors from adjacent germ layers
Wells, J. M., & Melton, D. A. (2000) · Development · doi:10.1242/dev.127.8.1563 · Cited by 366
Self-citationThe oligopeptide transporter (Pept-1) in human intestine: Biology and function
Adibi, S. (1997) · Gastroenterology · doi:10.1016/s0016-5085(97)70112-4 · Cited by 351
Peptide Transporters in the Intestine and the Kidney
Leibach, F. H., & Ganapathy, V. (1996) · Annual Review of Nutrition · doi:10.1146/annurev.nu.16.070196.000531 · Cited by 315
Yamada, T., Yoshikawa, M., Kanda, S., Kato, Y., Nakajima, Y., Ishizaka, S., & Tsunoda, Y. (2002) · STEM CELLS · doi:10.1634/stemcells.20-2-146 · Cited by 287
Vertebrate intestinal endoderm development
Spence, J. R., Lauf, R., & Shroyer, N. F. (2011) · Developmental Dynamics · doi:10.1002/dvdy.22540 · Cited by 219
Self-citationDessimoz, J., Opoka, R., Kordich, J. J., Grapin-Botton, A., & Wells, J. M. (2006) · Mechanisms of Development · doi:10.1016/j.mod.2005.10.001 · Cited by 189
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Organoids as an in vitro model of human development and disease
Fatehullah, A., Tan, S. H., & Barker, N. (2016) · Nature Cell Biology · doi:10.1038/ncb3312 · Cited by 1,508
Modelling human development and disease in pluripotent stem-cell-derived gastric organoids
McCracken, K. W., Catá, E. M., Crawford, C. M., Sinagoga, K. L., Schumacher, M., Rockich, B. E., Tsai, Y.-H., Mayhew, C. N., Spence, J. R., Zavros, Y., et al. (2014) · Nature · doi:10.1038/nature13863 · Cited by 980
Self-citationBroutier, L., Andersson-Rolf, A., Hindley, C. J., Boj, S. F., Clevers, H., Koo, B.-K., & Huch, M. (2016) · Nature Protocols · doi:10.1038/nprot.2016.097 · Cited by 834
In vitro generation of human pluripotent stem cell derived lung organoids
Dye, B. R., Hill, D. R., Ferguson, M. A., Tsai, Y.-H., Nagy, M. S., Dyal, R., Wells, J. M., Mayhew, C. N., Nattiv, R., Klein, O. D., et al. (2015) · eLife · doi:10.7554/elife.05098 · Cited by 819
Self-citationSynthetic hydrogels for human intestinal organoid generation and colonic wound repair
Cruz-Acuña, R., Quirós, M., Farkas, A. E., Dedhia, P. H., Huang, S., Siuda, D., García-Hernández, V., Miller, A. J., Spence, J. R., Nusrat, A., et al. (2017) · Nature Cell Biology · doi:10.1038/ncb3632 · Cited by 558
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Metadata
- Authors
- McCracken, K. W., Howell, J. C., Wells, J. M., & Spence, J. R.
- Venue
- Nature Protocols
- Published
- 2011-11-10
- Volume
- 6
- Issue
- 12
- Pages
- 1920-1928
- ISSN
- 1750-2799 (ISSN-L)
- 1754-2189
- OpenAlex
- W2055860761
- Cited by
- 437
- References
- 15
- FWCI
- 3.76
- Type
- journal article
- Language
- en
- Open access
- green
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