The DOI matches its Crossref record; 7 of the 7 references it cites were found in a registry or an index.
Comprehensive qPCR profiling of gene expression in single neuronal cells
Open accessAbstract
A major challenge in neuronal stem cell biology lies in characterization of lineage-specific reprogrammed human neuronal cells, a process that necessitates the use of an assay sensitive to the single-cell level. Single-cell gene profiling can provide definitive evidence regarding the conversion of one cell type into another at a high level of resolution. The protocol we describe uses Fluidigm Biomark dynamic arrays for high-throughput expression profiling from single neuronal cells, assaying up to 96 independent samples with up to 96 quantitative PCR (qPCR) probes (equivalent to 9,216 reactions) in a single experiment, which can be completed within 2–3 d. The protocol enables simple and cost-effective profiling of several hundred transcripts from a single cell, and it could have numerous utilities.
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The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments
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Projection-specific modulation of dopamine neuron synapses by aversive and rewarding stimuli
Lammel, S., Ion, D. I., Roeper, J., & Malenka, R. C. (2011) · Neuron · doi:10.1016/j.neuron.2011.03.025 · Cited by 766
Self-citationNeuroplasticity Mediated by Altered Gene Expression
McClung, C. A., & Nestler, E. J. (2008) · Neuropsychopharmacology · doi:10.1038/sj.npp.1301544 · Cited by 347
Single-Cell Transcriptional Analysis of Neuronal Progenitors
Tietjen, I., Rihel, J. M., Cao, Y., Koentges, G., Zakhary, L., & Dulac, C. (2003) · Neuron · doi:10.1016/s0896-6273(03)00229-0 · Cited by 260
Ginsberg, S. D., Elarova, I., Ruben, M., Tan, F., Counts, S. E., Eberwine, J. H., Trojanowski, J. Q., Hemby, S. E., Mufson, E. J., & Che, S. (2004) · Neurochemical Research · doi:10.1023/b:nere.0000023593.77052.f7 · Cited by 97
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Cited by
Zhang, Y., Chen, K., Sloan, S. A., Bennett, M. L., Scholze, A. R., O'Keeffe, S., Phatnani, H. P., Guarnieri, P., Caneda, C., Ruderisch, N., et al. (2014) · The Journal of Neuroscience · doi:10.1523/jneurosci.1860-14.2014 · Cited by 4,875
“We performed qRT-PCR with the Fluidigm BioMark system using methods modified from a previously described protocol (Citri et al., 2012).”
MethodBuettner, F., Natarajan, K. N., Casale, F. P., Proserpio, V., Scialdone, A., Theis, F. J., Teichmann, S. A., Marioni, J. C., & Stegle, O. (2015) · Nature Biotechnology · doi:10.1038/nbt.3102 · Cited by 1,136
Single-cell RNA-seq: advances and future challenges
Saliba, A.-E., Westermann, A. J., Gorski, S. A., & Vogel, J. (2014) · Nucleic Acids Research · doi:10.1093/nar/gku555 · Cited by 847
Using single nuclei for RNA-seq to capture the transcriptome of postmortem neurons
Krishnaswami, S. R., Grindberg, R. V., Novotny, M., Venepally, P., Lacar, B., Bhutani, K., Linker, S. B., Pham, S., Erwin, J. A., Miller, J. A., et al. (2016) · Nature Protocols · doi:10.1038/nprot.2016.015 · Cited by 445
iPS cells: a game changer for future medicine
Inoue, H., Nagata, N., Kurokawa, H., & Yamanaka, S. (2014) · The EMBO Journal · doi:10.1002/embj.201387098 · Cited by 405
Metadata
- Authors
- Citri, A., Pang, Z. P., Südhof, T. C., Wernig, M., & Malenka, R. C.
- Venue
- Nature Protocols
- Published
- 2011-12-22
- Volume
- 7
- Issue
- 1
- Pages
- 118-127
- ISSN
- 1750-2799 (ISSN-L)
- 1754-2189
- OpenAlex
- W2071104959
- Cited by
- 171
- References
- 7
- FWCI
- 6.70
- Type
- journal article
- Language
- en
- Open access
- green
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- Citri, A., Pang, Z. P., Südhof, T. C., Wernig, M., & Malenka, R. C. (2012). Comprehensive qPCR profiling of gene expression in single neuronal cells. Nature Protocols. https://doi.org/10.1038/nprot.2011.430
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