The DOI matches its Crossref record; 69 of the 69 references it cites were found in a registry or an index.
Second harmonic generation microscopy for quantitative analysis of collagen fibrillar structure
Open accessAbstract
Second-harmonic generation (SHG) microscopy has emerged as a powerful modality for imaging fibrillar collagen in a diverse range of tissues. Because of its underlying physical origin, it is highly sensitive to the collagen fibril/fiber structure, and, importantly, to changes that occur in diseases such as cancer, fibrosis and connective tissue disorders. We discuss how SHG can be used to obtain more structural information on the assembly of collagen in tissues than is possible by other microscopy techniques. We first provide an overview of the state of the art and the physical background of SHG microscopy, and then describe the optical modifications that need to be made to a laser-scanning microscope to enable the measurements. Crucial aspects for biomedical applications are the capabilities and limitations of the different experimental configurations. We estimate that the setup and calibration of the SHG instrument from its component parts will require 2-4 weeks, depending on the level of the user's experience.
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“second harmonic generation (SHG) microscopy has emerged as a label-free imaging modality capable of visualizing collagen-rich structures within intact spheroids [259,260].”
From Modeling Tumor Microenvironment Complexity In Vitro: Spheroids as Physiologically Relevant Tumor Models and Strategies for Their Analysis (2025) · cites this paper as [259]
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Cites
The Principles of Nonlinear Optics
Shen, Y. R. (1984) · Cited by 5,211
Label-Free Biomedical Imaging with High Sensitivity by Stimulated Raman Scattering Microscopy
Freudiger, C. W., Min, W., Saar, B. G., Lü, S., Holtom, G. R., He, C., Tsai, J. C.-S., Kang, J. X., & Xie, X. S. (2008) · Science · doi:10.1126/science.1165758 · Cited by 2,244
Collagen reorganization at the tumor-stromal interface facilitates local invasion
Provenzano, P. P., Eliceiri, K. W., Campbell, J. M., Inman, D. R., White, J. G., & Keely, P. J. (2006) · BMC Medicine · doi:10.1186/1741-7015-4-38 · Cited by 1,804
Zipfel, W. R., Williams, R. M., Christie, R. H., Nikitin, A. Y., Hyman, B. T., & Webb, W. W. (2003) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0832308100 · Cited by 1,712
Campagnola, P. J., & Loew, L. M. (2003) · Nature Biotechnology · doi:10.1038/nbt894 · Cited by 1,326
Self-citationAligned Collagen Is a Prognostic Signature for Survival in Human Breast Carcinoma
Conklin, M. W., Eickhoff, J. C., Riching, K. M., Pehlke, C. A., Eliceiri, K. W., Provenzano, P. P., Friedl, A., & Keely, P. J. (2011) · American Journal Of Pathology · doi:10.1016/j.ajpath.2010.11.076 · Cited by 1,321
Campagnola, P. J., Millard, A. C., Terasaki, M., Hoppe, P. E., Malone, C. J., & Mohler, W. A. (2002) · Biophysical Journal · doi:10.1016/s0006-3495(02)75414-3 · Cited by 922
Self-citationZoumi, A., Yeh, A. T., & Tromberg, B. J. (2002) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.172368799 · Cited by 872
Interpreting Second-Harmonic Generation Images of Collagen I Fibrils
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Building Collagen Molecules, Fibrils, and Suprafibrillar Structures
HULMES, D. J. S. (2002) · Journal of Structural Biology · doi:10.1006/jsbi.2002.4450 · Cited by 591
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Datta, R., Heaster, T. M., Sharick, J. T., Gillette, A. A., & Skala, M. C. (2020) · Journal of Biomedical Optics · doi:10.1117/1.jbo.25.7.071203 · Cited by 886
Bredfeldt, J. S., Liu, Y., Pehlke, C. A., Conklin, M. W., Szulczewski, J. M., Inman, D. R., Keely, P. J., Nowak, R. D., Mackie, T., & Eliceiri, K. W. (2014) · Journal of Biomedical Optics · doi:10.1117/1.jbo.19.1.016007 · Cited by 413
Collot, M., Fam, K. T., Ashokkumar, P., Faklaris, O., Galli, T., Danglot, L., & Klymchenko, A. S. (2018) · Journal of the American Chemical Society · doi:10.1021/jacs.7b12817 · Cited by 409
Marangoni, R. G., Korman, B. D., Wei, J., Wood, T. A., Graham, L. V., Whitfield, M. L., Scherer, P. E., Tourtellotte, W. G., & Varga, J. (2015) · Arthritis & Rheumatology · doi:10.1002/art.38990 · Cited by 337
Collagen: quantification, biomechanics and role of minor subtypes in cartilage
Bielajew, B. J., Hu, J. C. Y., & Athanasiou, K. A. (2020) · Nature Reviews Materials · doi:10.1038/s41578-020-0213-1 · Cited by 325
Metadata
- Authors
- Chen, X., Nadiarynkh, O., Plotnikov, S., & Campagnola, P. J.
- Affiliations
- University of Wisconsin–Madison (US)
- National Institutes of Health (US)
- Corresponding author
- Campagnola, Paul J
- Venue
- Nature Protocols
- Published
- 2012-03-08
- Volume
- 7
- Issue
- 4
- Pages
- 654-669
- ISSN
- 1750-2799 (ISSN-L)
- 1754-2189
- PMC
- PMC4337962
- OpenAlex
- W1996841581
- Cited by
- 985
- References
- 69
- Citations by year
- 2026: 67 · 2025: 81 · 2024: 91 · 2023: 81 · 2022: 80 · 2021: 93 · 2020: 101 · 2019: 73 · 2018: 82 · 2017: 71 · 2016: 51 · 2015: 44 · 2014: 38 · 2013: 23 · 2012: 7
- FWCI
- 16.39
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Nature Protocols (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Cancer Institute (R01CA136590, CA136590)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Advanced Fluorescence Microscopy Techniques
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Spectroscopy Techniques in Biomedical and Chemical Research
- Physical Sciences › Materials Science › Biomaterials › Collagen: Extraction and Characterization
- Keywords
- second harmonic generation microscopy, collagen fibrils, collagen fibril structure, collagen self-assembly, instrument calibration, connective tissue diseases, fibrosis, cancer, nonlinear optical imaging
- MeSH terms
- Calibration, Collagen, Microscopy, Optics and Photonics
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