Cited by 985Advanced Fluorescence Microscopy TechniquesSpectroscopy Techniques in Biomedical and Chemical ResearchCollagen: Extraction and Characterization

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Second harmonic generation microscopy for quantitative analysis of collagen fibrillar structure

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Chen, Xiyi, Nadiarynkh, Oleg, Plotnikov, Sergey, Campagnola, Paul J·2012·Nature Protocols

DOI 10.1038/nprot.2012.009PMC PMC4337962

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Abstract

Second-harmonic generation (SHG) microscopy has emerged as a powerful modality for imaging fibrillar collagen in a diverse range of tissues. Because of its underlying physical origin, it is highly sensitive to the collagen fibril/fiber structure, and, importantly, to changes that occur in diseases such as cancer, fibrosis and connective tissue disorders. We discuss how SHG can be used to obtain more structural information on the assembly of collagen in tissues than is possible by other microscopy techniques. We first provide an overview of the state of the art and the physical background of SHG microscopy, and then describe the optical modifications that need to be made to a laser-scanning microscope to enable the measurements. Crucial aspects for biomedical applications are the capabilities and limitations of the different experimental configurations. We estimate that the setup and calibration of the SHG instrument from its component parts will require 2-4 weeks, depending on the level of the user's experience.

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Authors: Chen, X., Nadiarynkh, O., Plotnikov, S., & Campagnola, P. J.
Affiliations: University of Wisconsin–Madison (US)
National Institutes of Health (US)
Corresponding author: Campagnola, Paul J
ORCID: Chen, Xiyi
Plotnikov, Sergey
Campagnola, Paul J
Venue: Nature Protocols
Publisher: Springer Science and Business Media LLC
Published: 2012-03-08
Volume: 7
Issue: 4
Pages: 654-669
ISSN: 1750-2799 (ISSN-L)
1754-2189
DOI: 10.1038/nprot.2012.009
PMC: PMC4337962
OpenAlex: W1996841581
Cited by: 985
References: 69
Citations by year: 2026: 67 · 2025: 81 · 2024: 91 · 2023: 81 · 2022: 80 · 2021: 93 · 2020: 101 · 2019: 73 · 2018: 82 · 2017: 71 · 2016: 51 · 2015: 44 · 2014: 38 · 2013: 23 · 2012: 7
FWCI: 16.39
Type: journal article
Language: en
Open access: green
www.ncbi.nlm.nih.gov
Licence: www.springer.com/tdm (tdm)
Locations: Nature Protocols (publishedVersion)
PubMed (publishedVersion)
Europe PMC (PubMed Central) (acceptedVersion)
PubMed Central (acceptedVersion)
Funders: National Cancer Institute (R01CA136590, CA136590)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Advanced Fluorescence Microscopy Techniques
Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Spectroscopy Techniques in Biomedical and Chemical Research
Physical Sciences › Materials Science › Biomaterials › Collagen: Extraction and Characterization
Keywords: second harmonic generation microscopy, collagen fibrils, collagen fibril structure, collagen self-assembly, instrument calibration, connective tissue diseases, fibrosis, cancer, nonlinear optical imaging
MeSH terms: Calibration, Collagen, Microscopy, Optics and Photonics

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Second harmonic generation microscopy for quantitative analysis of collagen fibrillar structure

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