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Assessment of mitochondrial respiratory chain enzymatic activities on tissues and cultured cells

Spinazzi, Marco, Casarin, Alberto, Pertegato, Vanessa, Salviati, Leonardo, Angelini, Corrado·2012·Nature Protocols

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Citations

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Research works printed but not linked: 29

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  4. Miro, O. et al. Mitochondrial DNA depletion and respiratory chain enzyme deficiencies are present in peripheral blood mononuclear cells of HIV-infected patients with HAART-related lipodystrophy. Antivir. Ther. 8, 333–338 (2003). · doi:10.1177/135965350300800410
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  7. Reisch, A.S. & Elpeleg, O. Biochemical assays for mitochondrial activity: assays of TCA cycle enzymes and PDHc. Methods Cell Biol. 80, 199–222 (2007). · DOI
  8. Villani, G. & Attardi, G. Polarographic assays of respiratory chain complex activity. Methods Cell Biol. 80, 121–133 (2007). · DOI
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  17. Salviati, L. et al. Copper supplementation restores cytochrome c oxidase activity in cultured cells from patients with SCO2 mutations. Biochem. J. 363, 321–327 (2002). · doi:10.1042/bj3630321
  18. Angelini, C. et al. Childhood encephalomyopathy with cytochrome c oxidase deficiency, ataxia, muscle wasting, and mental impairment. Neurology 36, 1048–1052 (1986). · doi:10.1212/wnl.36.8.1048
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  25. Janssen, A.J. et al. Spectrophotometric assay for complex I of the respiratory chain in tissue samples and cultured fibroblasts. Clin. Chem. 53, 729–734 (2007). · DOI · Paper page
  26. Moghaddas, S., Distler, A.M., Hoppel, C.L. & Lesnefsky, E.J. Quinol type compound in cytochrome c preparations leads to non-enzymatic reduction of cytochrome c during the measurement of complex III activity. Mitochondrion 8, 155–163 (2008). · DOI
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  29. Kirby, D.M., Thorburn, D.R., Turnbull, D.M. & Taylor, R.W. Biochemical assays of respiratory chain complex activity. Methods Cell Biol. 80, 93–119 (2007). · DOI · Paper page

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Metadata

Authors
Spinazzi, M., Casarin, A., Pertegato, V., Salviati, L., & Angelini, C.
Venue
Nature Protocols
Published
2012-05-31
Volume
7
Issue
6
Pages
1235-1246
ISSN
  • 1750-2799 (ISSN-L)
  • 1754-2189
OpenAlex
W2051476652
Cited by
983
References
6
FWCI
7.55
Type
journal article
Language
en
Open access
closed
Licence

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CoChat. (2026). Citation check: Assessment of mitochondrial respiratory chain enzymatic activities on tissues and cultured cells. https://app.cochat.ai/papers/10.1038/nprot.2012.058
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Spinazzi, M., Casarin, A., Pertegato, V., Salviati, L., & Angelini, C. (2012). Assessment of mitochondrial respiratory chain enzymatic activities on tissues and cultured cells. Nature Protocols. https://doi.org/10.1038/nprot.2012.058

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