The DOI matches its Crossref record; 47 of the 47 references it cites were found in a registry or an index.
MethylC-seq library preparation for base-resolution whole-genome bisulfite sequencing
Open accessAbstract
Current high-throughput DNA sequencing technologies enable acquisition of billions of data points through which myriad biological processes can be interrogated, including genetic variation, chromatin structure, gene expression patterns, small RNAs and protein–DNA interactions. Here we describe the MethylC-sequencing (MethylC-seq) library preparation method, a 2-d protocol that enables the genome-wide identification of cytosine DNA methylation states at single-base resolution. The technique involves fragmentation of genomic DNA followed by adapter ligation, bisulfite conversion and limited amplification using adapter-specific PCR primers in preparation for sequencing. To date, this protocol has been successfully applied to genomic DNA isolated from primary cell culture, sorted cells and fresh tissue from over a thousand plant and animal samples.
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Cites
Human DNA methylomes at base resolution show widespread epigenomic differences
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Self-citationEstablishing, maintaining and modifying DNA methylation patterns in plants and animals
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Highly Integrated Single-Base Resolution Maps of the Epigenome in Arabidopsis
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Self-citationShotgun bisulphite sequencing of the Arabidopsis genome reveals DNA methylation patterning
Cokus, S. J., Feng, S., Zhang, X., Chen, Z., Merriman, B., Haudenschild, C. D., Pradhan, S., Nelson, S. F., Pellegrini, M., & Jacobsen, S. E. (2008) · Nature · doi:10.1038/nature06745 · Cited by 2,256
High sensitivity mapping of methylated cytosines
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Global epigenomic reconfiguration during mammalian brain development
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Self-citationGenome-wide High-Resolution Mapping and Functional Analysis of DNA Methylation in Arabidopsis
Zhang, X., Yazaki, J., Sundaresan, A., Cokus, S., Chan, S. W.-L., Chen, H., Henderson, I. R., Shinn, P., Pellegrini, M., Jacobsen, S. E., et al. (2006) · Cell · doi:10.1016/j.cell.2006.08.003 · Cited by 1,815
Self-citationHigh density DNA methylation array with single CpG site resolution
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Cited by
Widespread natural variation of DNA methylation within angiosperms
Niederhuth, C. E., Bewick, A. J., Ji, L., Alabady, M. S., Kim, K. D., Li, Q., Rohr, N. A., Rambani, A., Burke, J. M., Udall, J. A., et al. (2016) · Genome Biology · doi:10.1186/s13059-016-1059-0 · Cited by 566
Self-citationSingle-cell methylomes identify neuronal subtypes and regulatory elements in mammalian cortex
Luo, C., Keown, C. L., Kurihara, L., Zhou, J., He, Y., Li, J., Castanon, R., Lucero, J., Nery, J. R., Sandoval, J. P., et al. (2017) · Science · doi:10.1126/science.aan3351 · Cited by 545
Self-citationCerebral Organoids Recapitulate Epigenomic Signatures of the Human Fetal Brain
Luo, C., Lancaster, M. A., Castanon, R., Nery, J. R., Knoblich, J. A., & Ecker, J. R. (2016) · Cell Reports · doi:10.1016/j.celrep.2016.12.001 · Cited by 370
Self-citationProfiling genome-wide DNA methylation
Yong, W.-S., Hsu, F.-M., & Chen, P.-Y. (2016) · Epigenetics & Chromatin · doi:10.1186/s13072-016-0075-3 · Cited by 374
Evolution of DNA Methylation across Insects
Bewick, A. J., Vogel, K. J., Moore, A. J., & Schmitz, R. J. (2016) · Molecular Biology and Evolution · doi:10.1093/molbev/msw264 · Cited by 353
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Metadata
- Authors
- Urich, M. A., Nery, J. R., Lister, R., Schmitz, R. J., & Ecker, J. R.
- Venue
- Nature Protocols
- Published
- 2015-02-18
- Volume
- 10
- Issue
- 3
- Pages
- 475-483
- ISSN
- 1750-2799 (ISSN-L)
- 1754-2189
- OpenAlex
- W1987293097
- Cited by
- 273
- References
- 47
- FWCI
- 13.25
- Type
- journal article
- Language
- en
- Open access
- green
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