Inexpensive, rapid prototyping of microfluidic devices using overhead transparencies and a laser print, cut and laminate fabrication method
Abstract
We describe a technique for fabricating microfluidic devices with complex multilayer architectures using a laser printer, a CO2 laser cutter, an office laminator and common overhead transparencies as a printable substrate via a laser print, cut and laminate (PCL) methodology. The printer toner serves three functions: (i) it defines the microfluidic architecture, which is printed on the overhead transparencies; (ii) it acts as the adhesive agent for the bonding of multiple transparency layers; and (iii) it provides, in its unmodified state, printable, hydrophobic 'valves' for fluidic flow control. By using common graphics software, e.g., CorelDRAW or AutoCAD, the protocol produces microfluidic devices with a design-to-device time of ∼40 min. Devices of any shape can be generated for an array of multistep assays, with colorimetric detection of molecular species ranging from small molecules to proteins. Channels with varying depths can be formed using multiple transparency layers in which a CO2 laser is used to remove the polyester from the channel sections of the internal layers. The simplicity of the protocol, availability of the equipment and substrate and cost-effective nature of the process make microfluidic devices available to those who might benefit most from expedited, microscale chemistry.
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Laser ablation allows for rapid materials patterning at small scales and is useful for engraving microfluidic channels into flexible plastic substrates.
“Laser ablation allows for rapid materials patterning at small scales and is useful for engraving microfluidic channels into flexible plastic substrates (39, 40).”
From Regional and correlative sweat analysis using high-throughput microfluidic sensing patches toward decoding sweat (2019) · cites this paper as [39]
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Self-citation
- Authors
- Thompson, B. L., Ouyang, Y., Duarte, G. R. M., Carrilho, E., Krauss, S. T., & Landers, J. P.
- Affiliations
- University of Virginia (US)
- Universidade Federal de Goiás (BR)
- Universidade de São Paulo (BR)
- Corresponding author
- Landers, James P
- Venue
- Nature Protocols
- Published
- 2015-05-14
- Volume
- 10
- Issue
- 6
- Pages
- 875-886
- ISSN
- 1750-2799 (ISSN-L)
- 1754-2189
- OpenAlex
- W1529713568
- Cited by
- 149
- References
- 41
- Citations by year
- 2026: 6 · 2025: 8 · 2024: 12 · 2023: 21 · 2022: 18 · 2021: 11 · 2020: 10 · 2019: 11 · 2018: 10 · 2017: 17 · 2016: 21 · 2015: 3 · 2014: 1
- FWCI
- 8.46
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Nature Protocols (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institute of Biomedical Imaging and Bioengineering (R01EB011591)
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › Microfluidic and Capillary Electrophoresis Applications
- Physical Sciences › Engineering › Biomedical Engineering › Biosensors and Analytical Detection
- Physical Sciences › Engineering › Biomedical Engineering › Innovative Microfluidic and Catalytic Techniques Innovation
- Keywords
- microfluidic fabrication, overhead transparencies, CO2 laser cutting, colorimetric sensing, low-cost prototyping
- MeSH terms
- Printing, Lasers, Gas, Lab-On-A-Chip Devices
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