Cited by 207Microfluidic and Capillary Electrophoresis ApplicationsAdvanced Proteomics Techniques and ApplicationsAnalytical Chemistry and Chromatography

Protein separation by capillary gel electrophoresis: A review

Open access
Zhu, Zaifang, Lu, Joann J., Liu, Shaorong·2012·Analytica Chimica Acta

Abstract

Capillary gel electrophoresis (CGE) has been used for protein separation for more than two decades. Due to the technology advancement, current CGE methods are becoming more and more robust and reliable for protein analysis, and some of the methods have been routinely used for the analysis of protein-based pharmaceuticals and quality controls. In light of this progress, we survey 147 papers related to CGE separations of proteins and present an overview of this technology. We first introduce briefly the early development of CGE. We then review the methodology, in which we specifically describe the matrices, coatings, and detection strategies used in CGE. CGE using microfabricated channels and incorporation of CGE with two-dimensional protein separations are also discussed in this section. We finally present a few representative applications of CGE for separating proteins in real-world samples.

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Crossref · 10.1016/j.aca.2011.10.022

Cites

149 of 149 references verified0 citation errors · 0 not checkable

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2 of the 151 references couldn’t be matched to a known paper.

Research works printed but not linked: 2

  1. Muramoto, 1977, Agric. Biol. Chem., 41, 2059
  2. Debaugnies, 2011, Acta Clin. Belg., 66, 165

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Authors
Zhu, Z., Lu, J. J., & Liu, S.
Affiliations
University of Oklahoma (US)
Corresponding author
Liu, Shaorong
Venue
Analytica Chimica Acta
Publisher
Elsevier BV
Published
2012-01
Volume
709
Pages
21-31
ISSN
0003-2670 (ISSN-L)
OpenAlex
W2039108689
Cited by
202
References
151
Citations by year
2026: 7 · 2025: 14 · 2024: 9 · 2023: 11 · 2022: 20 · 2021: 9 · 2020: 23 · 2019: 16 · 2018: 14 · 2017: 20 · 2016: 21 · 2015: 16 · 2014: 8 · 2013: 6 · 2012: 8
FWCI
0.57
Type
journal article
Language
en
Open access
Locations
  • Analytica Chimica Acta (publishedVersion)
  • PubMed (publishedVersion)
  • Europe PMC (PubMed Central) (acceptedVersion)
  • PubMed Central (acceptedVersion)
Funders
  • National Science Foundation (CHE 1011957)
  • National Institutes of Health (RO1 GM078592)
  • Oklahoma Center for the Advancement of Science and Technology
  • U.S. Department of Energy (SC0006351)
Topics
  • Physical Sciences › Engineering › Biomedical Engineering › Microfluidic and Capillary Electrophoresis Applications
  • Physical Sciences › Chemistry › Spectroscopy › Advanced Proteomics Techniques and Applications
  • Physical Sciences › Chemistry › Spectroscopy › Analytical Chemistry and Chromatography
Keywords
capillary gel electrophoresis, protein separation, capillary coating, detection methods, microfabricated channels, quality control
MeSH terms
Proteins, Spectrophotometry, Ultraviolet, Immunoblotting, Electrophoresis, Gel, Two-Dimensional, Electrophoresis, Capillary

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