The DOI matches its Crossref record; 179 of the 179 references it cites were found in a registry or an index.
Generation of human muscle fibers and satellite-like cells from human pluripotent stem cells in vitro
Open accessAbstract
Progress toward finding a cure for muscle diseases has been slow because of the absence of relevant cellular models and the lack of a reliable source of muscle progenitors for biomedical investigation. Here we report an optimized serum-free differentiation protocol to efficiently produce striated, millimeter-long muscle fibers together with satellite-like cells from human pluripotent stem cells (hPSCs) in vitro. By mimicking key signaling events leading to muscle formation in the embryo, in particular the dual modulation of Wnt and bone morphogenetic protein (BMP) pathway signaling, this directed differentiation protocol avoids the requirement for genetic modifications or cell sorting. Robust myogenesis can be achieved in vitro within 1 month by personnel experienced in hPSC culture. The differentiating culture can be subcultured to produce large amounts of myogenic progenitors amenable to numerous downstream applications. Beyond the study of myogenesis, this differentiation method offers an attractive platform for the development of relevant in vitro models of muscle dystrophies and drug screening strategies, as well as providing a source of cells for tissue engineering and cell therapy approaches.
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iMyoblasts for ex vivo and in vivo investigations of human myogenesis and disease modeling
Guo, D., Daman, K., Chen, J. J., Shi, M.-J., Yan, J., Matijasevic, Z., Rickard, A. M., Bennett, M. H., Kiselyov, A., Zhou, H., et al. (2022) · eLife · doi:10.7554/elife.70341 · Cited by 26
Metadata
- Authors
- Chal, J., Al Tanoury, Z., Hestin, M., Gobert, B., Aivio, S., Hick, A., Cherrier, T., Nesmith, A. P., Parker, K. K., & Pourquié, O.
- Venue
- Nature Protocols
- Published
- 2016-09-01
- Volume
- 11
- Issue
- 10
- Pages
- 1833-1850
- ISSN
- 1750-2799 (ISSN-L)
- 1754-2189
- OpenAlex
- W2517568305
- Cited by
- 299
- References
- 179
- FWCI
- 10.08
- Type
- journal article
- Language
- en
- Open access
- green
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