Basement membrane complexes with biological activity
Abstract
We have studied the reconstitution of basement membrane molecules from extracts prepared from the basement membrane of the EHS tumor. Under physiological conditions and in the presence of added type IV collagen and heparan sulfate proteoglycan, gellike structures form whose ultrastructure appears as interconnected thin sheets resembling the lamina dense zone of basement membrane. The major components of the reconstituted structures include laminin, type IV collagen, heparan sulfate proteoglycan, entactin, and nidogen. These components polymerize in constant proportions on reconstitution, suggesting that they interact in defined proportions. Molecular sieve studies on the soluble extract demonstrate that laminin, entactin, and nidogen are associated in large but dissociable complexes which may be a necessary intermediate in the deposition of basement membrane. The reconstituted matrix was biologically active and stimulated the growth and differentiation of certain cells.
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Matrigel, a murine Engelbreth-Holm-Swarm (EHS) sarcoma extract, supports iPSC pluripotency maintenance, neural induction, and multi-lineage differentiation through synergistic laminin, collagen IV, entactin, perlecan, and growth factor blends.
“Matrigel, a murine Engelbreth-Holm-Swarm (EHS) sarcoma extract, supports iPSC pluripotency maintenance, neural induction, and multi-linage differentiation through synergistic laminin, collagen IV, entactin, perlecan, and growth factor blends (Kleinman et al., 1986; Kleinman and Martin, 2005).”
From Challenges and solutions in transitioning to animal-free standards: a comprehensive analysis of components in human cell-based developmental neurotoxicity assays (2026) · cites this paper as Kleinman et al., 1986
Matrigel's multi-component bioactivity facilitates neurite outgrowth, synapse formation, and network maturation, key DNT endpoints.
“This multi-component bioactivity facilitates neurite outgrowth, synapse formation, and network maturation, key DNT endpoints, explaining its widespread adoption despite known batch-to-batch variability (Kleinman et al., 1986; Flanagan et al., 2006; Ma et al., 2008; Hughes et al., 2010; Li et al., 2014; Long and Huttner, 2019).”
From Challenges and solutions in transitioning to animal-free standards: a comprehensive analysis of components in human cell-based developmental neurotoxicity assays (2026) · cites this paper as Kleinman et al., 1986
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- Authors
- Kleinman, H. K., McGarvey, M. L., Hassell, J. R., Star, V. L., Cannon, F. B., Laurie, G. W., & Martin, G. R.
- Venue
- Biochemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 1986-01-28
- Volume
- 25
- Issue
- 2
- Pages
- 312-318
- ISSN
- 0006-2960
- 1520-4995
- Cited by
- 1,164
- References
- 1
- Language
- en
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