Differentiation of cardiomyocytes and generation of human engineered heart tissue
Breckwoldt, Kaja, Letuffe-Brenière, David, Mannhardt, Ingra, Schulze, Thomas, Ulmer, Bärbel, Werner, Tessa, Benzin, Anika, Klampe, Birgit, Reinsch, Marina C, Laufer, Sandra, Shibamiya, Aya, Prondzynski, Maksymilian, Mearini, Giulia, Schade, Dennis, Fuchs, Sigrid, Neuber, Christiane, Krämer, Elisabeth, Saleem, Umber, Schulze, Mirja L, Rodriguez, Marita L, Eschenhagen, Thomas, Hansen, Arne·2017·Nature Protocols
Cited by (272)
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- Designing a Bioreactor to Improve Data Acquisition and Model the Throughput of Engineered Cardiac TissuesCamille Van Neste, Karlo A. Wiley, Serena W. Chang, Joseph A. Borrello, Irene C. Turnbull, Kevin D. Costa · 2023 · Journal of Visualized Experiments
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- Altered endoplasmic reticulum calcium loading in human PLN-R14del cardiomyopathyWillem Borbein, Lukas Dahmlos, Umber Saleem, Marina C Reinsch, Ingke Braren, Thomas Schulze, Birgit Klampe, Friederike Cuello, Justus Stenzig, Thomas Eschenhagen A et al. · 2025 · Frontiers in Cell and Developmental Biology
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- Human Induced Pluripotent Stem Cells as Platform for Functional Examination of Cardiovascular Genetics in a DishRalf J. Dirschinger, Tatjana Dorn, Alessandra Moretti · 2019 · Cardiac and vascular biology
- Analysing Genetic Programs of Cell Differentiation to Study Cardiac Cell DiversificationZhixuan Wu, Sophie Shen, Yuliangzi Sun, Tessa R. Werner, Stephen T. Bradford, Nathan J. Palpant · 2022 · Springer eBooks
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- Recombinant annexin A6 promotes membrane repair in a stem cell derived-cardiomyocyte model of dystrophic cardiomyopathyDominic E. Fullenkamp, Alexander B. Willis, Jodi L Curtin, Ansel Philip Amaral, Sloane I. Harris, Paul W. Burridge, Alexis R. Demonbreun, Elizabeth M. McNally · 2022 · bioRxiv (Cold Spring Harbor Laboratory)
- A critical role of retinoic acid concentration for the induction of a fully human-like atrial phenotype in hiPSC-CMCarl Schulz, Muhammed Ikbal Sönmez, Julia Krause, Edzard Schwedhelm, Bangfen Pan, Dzenefa Alihodzic, Arne Hansen, Thomas Eschenhagen A, Torsten Christ · 2023 · bioRxiv (Cold Spring Harbor Laboratory)
- Efficient and reproducible generation of human iPSC-derived cardiomyocytes using a stirred bioreactorMaksymilian Prondzynski, Raul Hernandes Bortolin, Paul Berkson, Michael A. Trembley, Kevin Shani, Mason Sweat, Joshua Mayourian, Albert M. Cordoves, Nnaemeka J. Anyanwu, Yashasvi Tharani et al. · 2024 · bioRxiv (Cold Spring Harbor Laboratory)
- Metabolic cardiomyopathies: untangling clinical heterogeneity with human stem-cell derived modelsAdriana S Passadouro, Berith M. Balfoort, Mirjam Langeveld, Clara van Karnebeek, Jolanda van der Velden, Riekelt H. Houtkooper, Signe Mosegaard · 2025 · EMBO Molecular Medicine
- Investigating the Anticancer Effects of Sulforaphane in an In Vitro Coculture Model of Prostate Cancer Cells with Engineered Heart TissueJane In den Birken, Laura Rathjens, Hannah Münch, Tina Rohlfing, Κωνσταντίνα Σταθοπούλου, Alexandra Rhoden, Gunhild v. Amsberg, Thomas Eschenhagen A, Sergey A. Dyshlovoy, Friederike Cuello · 2025 · ACS Pharmacology & Translational Science
- Short-term electrical stimulation promotes partial functional and morphological maturation of human-induced pluripotent stem cell-derived cardiomyocytes enabling cardiotoxicity risk mitigation at early-stage drug discovery for cardiac contractility modulationHayato Miyoshi, Kaoru Morimura, Reiko Hara, Ritsuko Hori, Eriko Watanabe, Nobuyuki Mochizuki, Ayako Kamei, Rika Yamazaki, Katsuyuki Kazusa · 2026 · Toxicological Sciences
- Biologics for cardiovascular diseases: from bench to bedsideXiaochi Sun, Xinmeng Wang, Ruikun Jia, Jie Tang, X Y Zeng, Fulei Zhao, Fanlian Zeng, Nongyu Huang, Jiong Li, Kaijun Cui · 2026 · Signal Transduction and Targeted Therapy
- Human iPSC-derived engineered heart tissue model of diastolic dysfunction in heart failure with preserved ejection fractionHidenori Tani, Kotaro Haga, Taijun Moriwaki, Uikyu Bang, Shohei Fujii, Daichi SEKIGUCHI, Yuki Yamamoto, Kuniko Momoi, Masatoshi Ohno, M Nakamura et al. · 2026 · Cell stem cell
- Optogenetic tachypacing facilitates burst-induced arrhythmia in stem cell-derived human atrial engineered heart tissueKarl-Felix Müller, Bangfen Pan, Julius Ridder, Jessica Schrapers, Julius Hansen, Tandy M. Meier, Carl Schulz, Tim Stüdemann, Marta Lemme, Julia Krause et al. · 2026 · EP Europace
- Targeting PKGIα Cys 42 attenuates cardiac dysfunction in heart failure with preserved ejection fractionJie Su, Yue Zhao, Pierre Coleman, Xiaoping Yang, Mark Holt, Janice Raabe, Friederike Cuello, Ajay M. Shah, Michael J. Shattock, Min Zhang et al. · 2026 · Science Advances
- Modeling the Mechanical Characteristics of the Regenerated Tissues of Cardiovascular System With the Use of Patches Based on Tissue Engineering ConstructionsYuliya A. Terentyeva, Alexander Yu. Gerasimenko, Vladislav V. Shunaev, Olga E. Glukhova · 2019 · IEEE Conference of Russian Young Researchers in Electrical and Electronic Engineering
- Contractile and Genetic Characterization of Cardiac Constructs Engineered from Human Induced Pluripotent Stem Cells: Modeling of Tuberous Sclerosis Complex and the Effects of RapamycinVeniamin Y. Sidorov, Tatiana Nikolaevna Sidorova, Philip C. Samson, Ronald S. Reiserer, Clayton M. Britt, M. Diana Neely, Kevin C. Ess, John P. Wikswo · 2024 · Bioengineering
- Generation of a biallelic NRAP-knockout mutant from a human iPSC lineJanice Raabe, Vanessa Lewandowski, Sigrid Fuchs, Alexandra Hammerschmidt, Angelika Piasecki, Ellen Orthey, Elisabeth Krämer, Elisabeth Ehler, Friederike Cuello · 2025 · Stem Cell Research
- Nanotechnology in Generation and Biomedical Application of Induced Pluripotent Stem CellsHailin Tang, Mengjie Rui, Chuang Yu, Tao Chu, Chao Li, Zhenzhen Zhan, Hao Cao, Hangwen Li, Zhongmin Liu, Haifa Shen · 2018 · Nano LIFE
- Ruxolitinib clears CRYAB p.Arg120Gly aggregates through the ubiquitin-proteasome systemErda Alizoti, Leonie Ewald, Simona Parretta, Moritz Meyer-Jens, Ellen Orthey, Christian Conze, Lucie Carrier, Jeffrey M. Robbins, Sonia R. Singh · 2024 · bioRxiv (Cold Spring Harbor Laboratory)
- High rate triggers increased atrial release of BMP10, a biomarker for atrial fibrillation and stroke, which affects ventricular cardiomyocytesLaura C. Sommerfeld, Jessica Schrapers, KF Müller, L Bravo, Bente Siebels, Aaltje Maria Stella Stoter, Bangfen Pan, G Höppner, Christopher O’Shea, Julius Ridder et al. · 2025 · medRxiv
- RBFOX2 Autoregulation Promotes Cardiomyocyte Transcriptome Maturation and DifferentiationMengmeng Huang, Feria A. Ladha, Yunxia Wang, Michael A. Trembley, Huimin Yin, Rongbin Zheng, Maksymilian Prondzynski, Yashasvi Tharani, Alexander A. Akerberg, Stefan Aigner et al. · 2026 · Circulation Research
- OBSOLETE: Myocardial RepairThomas Eschenhagen · 2017 · Elsevier eBooks
- Cardiac tissue modelsJoycelyn K. Yip, Megan Laura McCain · 2018 · Elsevier eBooks
- Investigation of an Electrospun Nanofibrous, Degradable Polar Hydrophobic Ionic Polyurethane Patch for Cardiac Tissue RegenerationJennifer P. Y. Chan · 2018 · TSpace (University of Toronto)
- The role of MAP4K4 in cardiac muscle cell deathMicaela Jenkins · 2018 · Spiral (Imperial College London)
- Análise genética de pacientes portadores de cardiomiopatia arritmogênica do ventrículo direito (CAVD) e caracterização funcional em cardiomiócitos diferenciados (hiPSC-CM)Fanny Wulkan · 2019 · Digital Library of Theses and Dissertations (Universidade de São Paulo)
- Development of a myocardial infarction model by the design of a miniaturized 3D culture platformTom Boonen · 2019
- Geração de um modelo de células-tronco pluripotentes humanas para estudar os mecanismos moleculares de haploinsuficiência e dominância negativa da Síndrome de MarfanJuliana Borsoi · 2020 · Digital Library of Theses and Dissertations (Universidade de São Paulo)
- Epigenetics in cardiac development and human induced pluripotent stem cellsFederica De Majo, Gabriela M. Kuster, León J. De Windt · 2021 · Elsevier eBooks
- Directing cell differentiation in organ-on-chip devices through microarchitecture and supported lipid bilayersNicole J.L. Zeijen · 2021
- The morphology of the elements of the myocardial contractile apparatus question and research prospectsP.A. Kobeza · 2021 · Morphologia
- The Guinea Pig Model in Cardiac Regeneration Research; Current Tissue Engineering Approaches and Future DirectionsTim Stüdemann, Florian Weinberger · 2022 · Springer eBooks
- ABSTRACTS (BY NUMBER)Vijayaganapathy Vaithilingam, L.L. Reys, Mireille M.J.P.E. Sthijns, Timo Rademakers, Rick H. W. de Vries, Sami G. Mohammed, D De Bont, M Jetten, Eelco J.P. de Koning, Nizar I. Mourad et al. · 2022 · Tissue Engineering Part A
- Biomaterial Control of Differentiation of Human Embryonic Stem Cells and Induced Pluripotent Stem Cells2019 · Royal Society of Chemistry eBooks
- Myocardial RepairKaja Breckwoldt, Thomas Eschenhagen · 2017 · Elsevier eBooks
- Latifolin enhances both survival and cardiomyocyte differentiation of bone marrow mesenchymal stem cells on rat myocardial infarction modelNi Zhang, Xiao-Xiao Lai, Lanying Chen, Wenhong Li, Yingying Luo, An Li, Ronghua Liu, Xiaowei Meng · 2022 · Research Square
- Modelling, design, fabrication and characterization of engineered human myocardium made with melt electrowriting and cardiac cells derived from hiPSCsMaría Flandes‐Iparraguirre · 2023
- Functional modelling of arrhythmias with human cardiac organoidsMark L. Pocock · 2024 · Queensland University of Technology
- Success and Challenges with Models for Cardiac Translational ResearchRalf Franz Kettenhofen, Julia Christiane Neubauer · 2024 · Springer eBooks
- Biomaterials and stem cells for cardiac dysfunction treatmentsDeepti Singh, Pierre C. Dromel · 2024 · Elsevier eBooks
- Success and Challenges with Models for Cardiac Translational ResearchRalf Franz Kettenhofen, Julia Christiane Neubauer · 2024 · Springer eBooks
- Advances in human induced pluripotent stem cell (hiPSC)-based disease modelling in cardiogeneticsSandra Hoffmann, Timon Seeger · 2025 · Medizinische Genetik
- Targeting C42 in PKGIα alleviates diastolic dysfunction in HFpEFJie Su, Min Zhang, Xiaoping Yang, Mark Holt, Janice Raabe, Friederike Cuello, Ajay M. Shah, Michael J. Shattock, Joseph Robert Burgoyne · 2025 · bioRxiv (Cold Spring Harbor Laboratory)
- Oxygen Handling Practices for in Vitro Human Pluripotent Stem Cell Cardiomyocyte Differentiation: A Scoping Literature ReviewLisse Frans, Jeffrey Aalders, Jolanda van Hengel, Alain J. Labro · 2025 · Stem Cell Reviews and Reports
- Positive inotropic effects of serotonin in atrial EHT: further proof for an atrial phenotype?Muhammed Ikbal Sönmez, Margaret Nandudu, Paul Brunnbauer, Carl Schulz, Birgit Klampe, Thomas Schulze, Arne Hansen, Thomas Eschenhagen A, Torsten Christ · 2025 · Naunyn-Schmiedeberg s Archives of Pharmacology
- Generation of a NRAP-overexpressing mutant from a human iPSC lineJanice Raabe, Sigrid Fuchs, Christa Augustin, Alexandra Hammerschmidt, Elisabeth Krämer, Ellen Orthey, Elisabeth Ehler, Friederike Cuello · 2025 · Stem Cell Research
- Field potential duration and its variability as essential parameters for revealing proarrhythmia: problematic aspects of analysis in cardiomyocytes derived from human pluripotent stem cellsMartin Král, Olga Švecová, Pavel Jurák, Josef Halámek, Milena Šimurdová, Jiří Šimurda, Markéta Bébarová · 2025 · Progress in Biophysics and Molecular Biology
- The conjugation-resistant bile acid norUDCA cures liver fibrosis but impairs systemic energy metabolismJöerg Heeren, Ioannis Evangelakos, Esther Verkade, Folkert Kuipers, Julia Sarah Rohde, Markus Heine, Anna Worthmann, Sebastian Graute, Manka M. Fuh, Karthikeyan Gunasekaran et al. · 2025 · Research Square
- Additional file 1 of An arrhythmogenic metabolite in atrial fibrillationJulia Krause, Nickel, Alexander, Alexandra Madsen, Hamish M. Aitken‐Buck, Aaltje Maria Stella Stoter, Jessica Schrapers, Ojeda, Francisco, Kira Geiger, Kern, Melanie, Kohlhaas, Michael et al. · 2024 · Figshare
- Additional file 2 of NRF2 is required for structural and metabolic maturation of human induced pluripotent stem cell-derived ardiomyocytesZhang, Xinyuan, Ye, Liang, Xu, Hao, Zhou, Qin, Tan, Bin, Qin Yi, Yan, Liang, Min Xie, Yin Zhang, Tian, Jie et al. · 2021 · Figshare
- Engineering Human 3D Cardiac Tissues for Predictive Functional Drug ScreeningElisheva Baruch, Daniel Rosner, Elisabeth Riska, Moran Yadid, Assaf Shapira, Tal Dvir · 2025 · Pharmaceutics
- Advances in Internal Organogenesis: Differentiation and Morphogenesis of Human Ventral Cavity OrgansMuhammad Iqbal Qureshi, Afsar Ali Mian, Irfan Khan · 2026 · Stem Cell Reviews and Reports
- Simtuzumab Attenuates Loxl2-Mediated Extracellular Matrix Remodeling and Preserves Cardiac Function in LMNA Mutation-Induced Dilated CardiomyopathyMarie Kervella, Charlotta Sophie Behrens, Cécile Peccate, Zoheir Guesmia, Fiorella Carla Grandi, Nathalie Mougenot, Anne Forand, Azzouz Charrabi, Guy Brochier, Ramaroson Andriantsitohaina et al. · 2026 · Circulation Heart Failure
- JAK1/2 inhibitor ruxolitinib reduces aggregates in cardiac proteinopathyErda Alizoti, Leonie Ewald, Simona Parretta, Jacob J K March, Moritz Meyer-Jens, Ellen Orthey, Christian Conze, Lucie Carrier, Jeffrey M. Robbins, Sonia R. Singh · 2026 · EMBO Molecular Medicine
- The conjugation-resistant bile acid norUDCA cures liver fibrosis but impairs systemic energy metabolismIoannis Evangelakos, Esther Verkade, Julia K. Rohde, Alex Zaufel, Martin Vargek, Markus Heine, Anna Worthmann, Sebastian Graute, Marceline Manka Fuh, Karthikeyan Gunasekaran et al. · 2026 · Molecular Metabolism
- Myocardial RepairKaja Breckwoldt, Thomas Eschenhagen · 2026 · Elsevier eBooks
- Length-dependent hypercontractility decoupled from cellular adaptations is the initial phenotype in MYBPC3-haploinsufficient iPSC-derived engineered cardiac tissuesTomi Tuomainen, Maike Mona Širinov, Mina Rabiee, Eloi Schmauch, Jussi T. Koivumäki, Kyriaki Galani, M Kellis, Johanna Kuusisto, Robert S. Leigh, Nikolay Naumenko et al. · 2026 · bioRxiv (Cold Spring Harbor Laboratory)
- Mitochondria‐endoplasmic reticulum organelle glue as maturation promoter for origination/dimensional dual‐cross cardiomyocytesWei Tang, Hao Sun, Chenqi Duan, Jiajia Chang, Yuxing Wang, Ming Zhao, Huiyue Zhao, Shan Lu, Yang Yang, Da Huo · 2026 · BMEMat
- Mavacamten shows broad benefit in human and mouse models of MYBPC3-related hypertrophic cardiomyopathyLaura Sen-Martín, Ángel Fernández‐Trasancos, Miguel Ángel López-Unzu, Agata Bak, Magdalena Zafra-Castellano, Rajiven Srikantharajah, Divya Pathak, Annika J. Klotz, Michel N. Kuehn, Alessia Ferrarini et al. · 2026 · Nature Cardiovascular Research
- Xeno-Free Peptide-Functionalized Hydrogels Support hiPSC Encapsulation and In Situ Differentiation into Structurally Mature CardiomyocytesMohammadjafar Hashemi, Nongmaithem Debeni Devi, Yasaman Kargar Gaz Kooh, Charlotte Chen, Bahareh Bahmani, Ganesh Malayath, Julia Victor, Nathaniel Huebsch · 2026 · bioRxiv (Cold Spring Harbor Laboratory)
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- Phenotype rescue through the restoration of full-length dystrophin using CRISPR/Cas9 genome editing in Duchenne muscular dystrophy patient-derived iPSCs carrying the deletion of two exonsDavide Rovina, Sofia Milasi, Giusy Di Giuseppe, Josè Manuel Pioner, Marianna Langione, Lucrezia Giammarino, Cecilia Ferrantini, Mattia Chiesa, Francesco Canonico, Domenico D’Amario et al. · 2026 · Genome Medicine
- Dynamic BMP10 Release Reflects Atrial Fibrillation Burden in Human Atrial Engineered Heart TissueLukas von Hacht, Tandy M. Meier, Julius Ridder, Jessica Schrapers, Ann‐Kristin Afflerbach, Marc N. Hirt, Arne Hansen, Paulus F. Kirchhof, Thomas Eschenhagen A, Justus Stenzig et al. · 2026 · bioRxiv (Cold Spring Harbor Laboratory)
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- BindingDB Entry 50017258: Phenotypic screen identifies FOXO inhibitor to counteract maturation and promote expansion of human iPS cell-derived cardiomyocytes.BindingDB · 2023 · UC San Diego