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Cited by 345

The DOI matches its Crossref record; 139 of the 139 references it cites were found in a registry or an index.

Microfluidics: reframing biological enquiry

Open access
Duncombe, Todd A., Tentori, Augusto M., Herr, Amy E.·2015·Nature Reviews Molecular Cell Biology

Abstract

The underlying physical properties of microfluidic tools have led to new biological insights through the development of microsystems that can manipulate, mimic and measure biology at a resolution that has not been possible with macroscale tools. Microsystems readily handle sub-microlitre volumes, precisely route predictable laminar fluid flows and match both perturbations and measurements to the length scales and timescales of biological systems. The advent of fabrication techniques that do not require highly specialized engineering facilities is fuelling the broad dissemination of microfluidic systems and their adaptation to specific biological questions. We describe how our understanding of molecular and cell biology is being and will continue to be advanced by precision microfluidic approaches and posit that microfluidic tools — in conjunction with advanced imaging, bioinformatics and molecular biology approaches — will transform biology into a precision science.

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Citations

Cites

Show all 139 references

Research works printed but not linked: 9

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  3. Akagi, J. et al. Integrated chip-based physiometer for automated fish embryo toxicity biotests in pharmaceutical screening and ecotoxicology. Cytometry A 85, 537–547 (2014). · doi:10.1002/cyto.a.22464
  4. Akagi, J. et al. Opensource lab-on-a-chip physiometer for accelerated zebrafish embryo biotests. Curr. Protoc. Cytom. 67, 9.44.1–9.44.16 (2014). · doi:10.1002/0471142956.cy0944s67
  5. Zheng, C. et al. Fish in chips: an automated microfluidic device to study drug dynamics in vivo using zebrafish embryos. Chem. Commun. 50, 981–984 (2014). · doi:10.1039/c3cc47285j
  6. Chiang, Y.-Y. & West, J. Ultrafast cell switching for recording cell surface transitions: new insights into epidermal growth factor receptor signalling. Lab Chip 13, 1031–1034 (2013). · doi:10.1039/c3lc41297k
  7. Fazelinia, H., Xu, M., Cheng, H. & Roder, H. Ultrafast hydrogen exchange reveals specific structural events during the initial stages of folding of cytochrome c. J. Am. Chem. Soc. 136, 733–740 (2014). Ultra-fast microfluidic mixing for hydrogen–deuterium exchange enabled researchers to determine that decreased chain dimensions during the early stages of cytochrome c folding are due to specific α-helix interactions and not a general hydrophobic collapse of the protein. · doi:10.1021/ja410437d
  8. Granieri, L., Baret, J. C., Griffiths, A. D. & Merten, C. A. High-throughput screening of enzymes by retroviral display using droplet-based microfluidics. Chem. Biol. 17, 229–235 (2010). · doi:10.1016/j.chembiol.2010.02.011
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Metadata

Authors
Duncombe, T. A., Tentori, A. M., & Herr, A. E.
Venue
Nature Reviews Molecular Cell Biology
Published
2015-08-21
Volume
16
Issue
9
Pages
554-567
ISSN
  • 1471-0072 (ISSN-L)
  • 1471-0080
OpenAlex
W1179069838
Cited by
345
References
139
FWCI
3.04
Type
journal article
Language
en
Open access
green
Licence
www.springer.com/tdm (tdm)

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Duncombe, T. A., Tentori, A. M., & Herr, A. E. (2015). Microfluidics: reframing biological enquiry. Nature Reviews Molecular Cell Biology. https://doi.org/10.1038/nrm4041

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