Cited by 726Protein Structure and DynamicsChemical Synthesis and AnalysisRNA and protein synthesis mechanisms

Elucidating the folding problem of helical peptides using empirical parameters

Muñoz, Victor, Serrano, Luis·1994·Nature Structural & Molecular Biology

Abstract

Using an empirical analysis of experimental data we have estimated a set of energy contributions which accounts for the stability of isolated alpha-helices. With this database and an algorithm based on statistical mechanics, we describe the average helical behaviour in solution of 323 peptides and the helicity per residue of those peptides analyzed by nuclear magnetic resonance. Moreover the algorithm successfully detects the alpha-helical tendency, in solution, of a peptide corresponding to a beta-strand of ubiquitin.

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Crossref · 10.1038/nsb0694-399

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  • Calculations of the percentage of helicity of the CC-Hex*-L24E monomer with Agadir show 13% helicity at pH 2 and 9% helicity at pH 8.5, with intermediate helicities in the pH range 2-8.5.

    “indeed, calculations of the percentage of helicity of the CC-Hex*-L24E monomer with Agadir114−118 show 13% helicity at pH 2 and 9% helicity at pH 8.5, with intermediate helicities in the pH range 2–8.5.”

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Authors
Muñoz, V., & Serrano, L.
Affiliations
European Molecular Biology Laboratory (DE)
Venue
Nature Structural & Molecular Biology
Published
1994-06
Volume
1
Issue
6
Pages
399-409
ISSN
  • 1545-9985 (ISSN-L)
  • 1545-9993
OpenAlex
W2083939457
Cited by
721
References
94
Citations by year
2026: 12 · 2025: 9 · 2024: 17 · 2023: 19 · 2022: 19 · 2021: 16 · 2020: 20 · 2019: 14 · 2018: 26 · 2017: 20 · 2016: 15 · 2015: 20 · 2014: 31 · 2013: 29 · 2012: 23
FWCI
8.52
Type
journal article
Language
en
Open access
closed
Locations
  • Nature Structural & Molecular Biology (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Structure and Dynamics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Chemical Synthesis and Analysis
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
Keywords
helical peptides, alpha-helix stability, peptide folding, nuclear magnetic resonance
MeSH terms
Algorithms, Amino Acid Sequence, Circular Dichroism, Hydrogen Bonding, Molecular Sequence Data, Magnetic Resonance Spectroscopy, Solutions, Ubiquitins, Protein Structure, Secondary, Protein Folding

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