Stabilization of alpha-helical structures in short peptides via end capping
Open accessAbstract
The alpha-helix-stabilizing effect of different amino acid residues at the helical termini of short peptides in aqueous solution has been determined. Several dodecapeptides containing alanine, asparagine, aspartate, glutamine, glutamate, and serine at the amino terminus and arginine, lysine, and alanine at the carboxyl terminus were synthesized, and the alpha-helical content of each peptide was measured by using circular dichroism spectroscopy. The trend in alpha-helix-inducing ability of these amino acids was found to be as follows: aspartate > asparagine > serine > glutamate > glutamine > alanine at the amino terminus and arginine > lysine > alanine at the carboxyl terminus. Our results agree with the Presta and Rose hypothesis [Presta, L. G. & Rose, G. D. (1988) Science 240, 1632-1641] on the role of end capping in helix stabilization.
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- Authors
- Forood, B., Feliciano, E. J., & Nambiar, K. P.
- Affiliations
- University of California, Davis (US)
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 1993-02
- Volume
- 90
- Issue
- 3
- Pages
- 838-842
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC45765
- OpenAlex
- W2076072268
- Cited by
- 235
- References
- 23
- Citations by year
- 2026: 4 · 2025: 2 · 2024: 7 · 2023: 6 · 2022: 11 · 2021: 7 · 2020: 5 · 2019: 7 · 2018: 7 · 2017: 9 · 2016: 8 · 2015: 6 · 2014: 8 · 2013: 8 · 2012: 7
- FWCI
- 4.13
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health (5r29gm039822-05)
- National Institute of General Medical Sciences (R29 GM 39822)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Structure and Dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Chemical Synthesis and Analysis
- Physical Sciences › Chemistry › Spectroscopy › Molecular spectroscopy and chirality
- Keywords
- alpha-helix stabilization, end-capping, small peptides, amino acid side chains, dodecapeptide, aspartic acid, asparagine, serine, glutamate, glutamine, L-arginine, lysine, helix content
- MeSH terms
- Amino Acid Sequence, Amino Acids, Circular Dichroism, Cold Temperature, Fluorides, Hot Temperature, Hydrogen-Ion Concentration, Molecular Sequence Data, Oligopeptides, Potassium, Spectrophotometry, Ultraviolet, Trifluoroethanol, Protein Structure, Secondary, Potassium Compounds
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