The DOI matches its Crossref record; 44 of the 44 references it cites were found in a registry or an index.
Structural basis of ligand binding by a c-di-GMP riboswitch
Open accessAbstract
The second messenger signaling molecule bis-(3′-5′)-cyclic dimeric guanosine monophosphate (c-di-GMP) regulates many processes in bacteria, including motility, pathogenesis and biofilm formation. c-di-GMP–binding riboswitches are important downstream targets in this signaling pathway. Here we report the crystal structure, at 2.7 Å resolution, of a c-di-GMP riboswitch aptamer from Vibrio cholerae bound to c-di-GMP, showing that the ligand binds within a three-helix junction that involves base-pairing and extensive base-stacking. The symmetric c-di-GMP is recognized asymmetrically with respect to both the bases and the backbone. A mutant aptamer was engineered that preferentially binds the candidate signaling molecule c-di-AMP over c-di-GMP. Kinetic and structural data suggest that genetic regulation by the c-di-GMP riboswitch is kinetically controlled and that gene expression is modulated through the stabilization of a previously unidentified P1 helix, illustrating a direct mechanism for c-di-GMP signaling.
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Cited by
Gao, P., Ascano, M., Wu, Y., Barchet, W., Gaffney, B. L., Zillinger, T., Serganov, A. A., Liu, Y., Jones, R. A., Hartmann, G., et al. (2013) · Cell · doi:10.1016/j.cell.2013.04.046 · Cited by 1,101
Prospects for riboswitch discovery and analysis
Breaker, R. R. (2011) · Molecular Cell · doi:10.1016/j.molcel.2011.08.024 · Cited by 464
Self-citationMetabolite recognition principles and molecular mechanisms underlying riboswitch function
Serganov, A., & Patel, D. J. (2012) · Annual Review of Biophysics · doi:10.1146/annurev-biophys-101211-113224 · Cited by 160
c-di-AMP binds the ydaO riboswitch in two pseudo-symmetry–related pockets
Ren, A., & Patel, D. J. (2014) · Nature Chemical Biology · doi:10.1038/nchembio.1606 · Cited by 104
Themes and variations in riboswitch structure and function
Peselis, A., & Serganov, A. (2014) · Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms · doi:10.1016/j.bbagrm.2014.02.012 · Cited by 102
Metadata
- Authors
- Smith, K. D., Lipchock, S. V., Ames, T. D., Wang, J., Breaker, R. R., & Strobel, S. A.
- Venue
- Nature Structural & Molecular Biology
- Published
- 2009-11-08
- Volume
- 16
- Issue
- 12
- Pages
- 1218-1223
- ISSN
- 1545-9985 (ISSN-L)
- 1545-9993
- OpenAlex
- W2154379876
- Cited by
- 284
- References
- 44
- FWCI
- 10.92
- Type
- journal article
- Language
- en
- Open access
- green
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- Smith, K. D., Lipchock, S. V., Ames, T. D., Wang, J., Breaker, R. R., & Strobel, S. A. (2009). Structural basis of ligand binding by a c-di-GMP riboswitch. Nature Structural & Molecular Biology. https://doi.org/10.1038/nsmb.1702
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