The DOI matches its Crossref record; 5 of the 5 references it cites were found in a registry or an index.
R loops regulate promoter-proximal chromatin architecture and cellular differentiation
Open accessAbstract
Numerous chromatin-remodeling factors are regulated by interactions with RNA, although the contexts and functions of RNA binding are poorly understood. Here we show that R loops, RNA-DNA hybrids consisting of nascent transcripts hybridized to template DNA, modulate the binding of two key chromatin-regulatory complexes, Tip60–p400 and polycomb repressive complex 2 (PRC2) in mouse embryonic stem cells (ESCs). Like PRC2, the Tip60–p400 histone acetyltransferase complex binds to nascent transcripts; however, transcription promotes chromatin binding of Tip60–p400 but not PRC2. Interestingly, we observed higher Tip60–p400 and lower PRC2 levels at genes marked by promoter-proximal R loops. Furthermore, disruption of R loops broadly decreased Tip60–p400 occupancy and increased PRC2 occupancy genome wide. In agreement with these alterations, ESCs partially depleted of R loops exhibited impaired differentiation. These results show that R loops act both positively and negatively in modulating the recruitment of key pluripotency regulators.
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The Drosophila Fab-7 Chromosomal Element Conveys Epigenetic Inheritance during Mitosis and Meiosis
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García-Muse, T., & Aguilera, A. (2019) · Cell · doi:10.1016/j.cell.2019.08.055 · Cited by 697
A circRNA from SEPALLATA3 regulates splicing of its cognate mRNA through R-loop formation
Conn, V. M., Hugouvieux, V., Nayak, A., Conos, S. A., Capovilla, G., Cildir, G., Jourdain, A., Tergaonkar, V., Schmid, M., Zubieta, C., et al. (2017) · Nature Plants · doi:10.1038/nplants.2017.53 · Cited by 584
Chen, L., Chen, J.-Y., Zhang, X., Gu, Y., Xiao, R., Shao, C., Tang, P., Qian, H., Luo, D., Li, H., et al. (2017) · Molecular Cell · doi:10.1016/j.molcel.2017.10.008 · Cited by 375
A NPAS4–NuA4 complex couples synaptic activity to DNA repair
Pollina, E. A., Gilliam, D. T., Landau, A. T., Lin, C., Pajarillo, N., Davis, C. P., Harmin, D. A., Yap, E.-L., Vogel, I. R., Griffith, E. C., et al. (2023) · Nature · doi:10.1038/s41586-023-05711-7 · Cited by 125
Metadata
- Authors
- Chen, P. B., Chen, H. V., Acharya, D., Rando, O. J., & Fazzio, T. G.
- Venue
- Nature Structural & Molecular Biology
- Published
- 2015-11-09
- Volume
- 22
- Issue
- 12
- Pages
- 999-1007
- ISSN
- 1545-9985 (ISSN-L)
- 1545-9993
- OpenAlex
- W2194327633
- Cited by
- 196
- References
- 5
- FWCI
- 4.76
- Type
- journal article
- Language
- en
- Open access
- green
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