The DOI matches its Crossref record; 6 of the 6 references it cites were found in a registry or an index.
An antimicrobial peptide that inhibits translation by trapping release factors on the ribosome
Open accessAbstract
Many antibiotics stop bacterial growth by inhibiting different steps of protein synthesis. However, no specific inhibitors of translation termination are known. Proline-rich antimicrobial peptides, a component of the antibacterial defense system of multicellular organisms, interfere with bacterial growth by inhibiting translation. Here we show that Api137, a derivative of the insect-produced antimicrobial peptide apidaecin, arrests terminating ribosomes using a unique mechanism of action. Api137 binds to the Escherichia coli ribosome and traps release factor (RF) RF1 or RF2 subsequent to the release of the nascent polypeptide chain. A high-resolution cryo-EM structure of the ribosome complexed with RF1 and Api137 reveals the molecular interactions that lead to RF trapping. Api137-mediated depletion of the cellular pool of free release factors causes the majority of ribosomes to stall at stop codons before polypeptide release, thereby resulting in a global shutdown of translation termination.
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Lazzaro, B. P., Zasloff, M., & Rolff, J. (2020) · Science · doi:10.1126/science.aau5480 · Cited by 1,069
Antimicrobial peptides: mechanism of action, activity and clinical potential
Zhang, Q.-Y., Yan, Z.-B., Meng, Y.-M., Hong, X.-Y., Shao, G., Ma, J.-J., Cheng, X.-R., Liu, J., Kang, J., & Fu, C.-Y. (2021) · Military Medical Research · doi:10.1186/s40779-021-00343-2 · Cited by 1,065
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Xuan, J., Feng, W., Wang, J., Wang, R., Zhang, B., Bo, L., Chen, Z.-S., Yang, H., & Sun, L. (2023) · Drug Resistance Updates · doi:10.1016/j.drup.2023.100954 · Cited by 665
Identification of antimicrobial peptides from the human gut microbiome using deep learning
Ma, Y., Guo, Z., Xia, B., Zhang, Y., Liu, X., Yu, Y., Tang, N., Tong, X., Wang, M., Ye, X., et al. (2022) · Nature Biotechnology · doi:10.1038/s41587-022-01226-0 · Cited by 490
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Metadata
- Authors
- Florin, T., Maracci, C., Graf, M., Karki, P., Klepacki, D., Berninghausen, O., Beckmann, R., Vázquez-Laslop, N., Wilson, D. N., Rodnina, M. V., & Mankin, A. S.
- Venue
- Nature Structural & Molecular Biology
- Published
- 2017-07-24
- Volume
- 24
- Issue
- 9
- Pages
- 752-757
- ISSN
- 1545-9985 (ISSN-L)
- 1545-9993
- OpenAlex
- W2738678411
- Cited by
- 190
- References
- 6
- FWCI
- 5.34
- Type
- journal article
- Language
- en
- Open access
- green
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- Florin, T., Maracci, C., Graf, M., Karki, P., Klepacki, D., Berninghausen, O., Beckmann, R., Vázquez-Laslop, N., Wilson, D. N., Rodnina, M. V., & Mankin, A. S. (2017). An antimicrobial peptide that inhibits translation by trapping release factors on the ribosome. Nature Structural & Molecular Biology. https://doi.org/10.1038/nsmb.3439
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