Cited by 432Alzheimer's disease research and treatmentsPluripotent Stem Cells ResearchDementia and Cognitive Impairment Research

The DOI matches its Crossref record; 33 of the 33 references it cites were found in a registry or an index.

Modeling amyloid beta and tau pathology in human cerebral organoids

Open access

Gonzalez, Cesar, Armijo, Enrique, Bravo-Alegria, Javiera, Becerra-Calixto, Andrea, Mays, Charles E., Soto, Claudio·2018·Molecular Psychiatry

DOI 10.1038/s41380-018-0229-8PMC PMC6594704

Open this paper in CoChat — free account

Abstract

The typical abnormalities observed in the brain of Alzheimer's disease (AD) patients include synaptic alterations, neuronal death, brain inflammation, and the accumulation of protein aggregates in the form of amyloid plaques and neurofibrillary tangles. Despite the development of many animal and in vitro models for AD, there is a lack of an experimental approach that fully recapitulates essential aspects of the disease in human cells. Here, we report the generation of a new model to study AD, consisting of cerebral organoids (COs) produced from human-induced pluripotent stem cells (iPSCs). Under our experimental conditions, COs grow to form three-dimensional (3D) structures containing neural areas with cortical-like organization. Analysis of COs by histological and biochemical methods revealed that organoids produced from iPSCs derived from patients affected by familial AD or Down syndrome (DS) spontaneously develop over time pathological features of AD, including accumulation of structures highly reminiscent to amyloid plaques and neurofibrillary tangles. These pathological abnormalities were not observed in COs generated from various controls, including human iPSCs from healthy individuals, human iPSCs from patients affected by Creutzfeldt-Jakob disease, mouse embryonic stem cells (ESCs), or mouse iPSCs. These findings enable modeling genetic AD in a human cellular context in a 3D cortical-like tissue developed in vitro from patient-specific stem cells. This system provides a more relevant disease model compared to pre-existing methods and offers a new platform for discovery of novel targets and screening of drugs for therapeutic intervention.

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

Integrity signals

Registry

Crossref · 10.1038/s41380-018-0229-8

Cites

33 of 33 references verified0 citation errors · 0 not checkable

Statistics

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Heat Shock Protein A12B Protects Vascular Endothelial Cells Against Sepsis-Induced Acute Lung Injury in Mice
Chen, Y., Wang, L., Kang, Q., Zhang, X., Yu, G., Wan, X., Wang, J., & Zhu, K. (2017) · Cellular Physiology and Biochemistry · doi:10.1159/000477308 · Cited by 10,730
Cerebral organoids model human brain development and microcephaly
Lancaster, M. A., Renner, M., Martin, C.-A., Wenzel, D., Bicknell, L. S., Hurles, M. E., Homfray, T., Penninger, J. M., Jackson, A. P., & Knoblich, J. A. (2013) · Nature · doi:10.1038/nature12517 · Cited by 5,372
Alzheimer’s disease
Querfurth, H. W., & LaFerla, F. M. (2010) · New England Journal of Medicine · doi:10.1056/nejmra0909142 · Cited by 4,569
Cloning of a gene bearing missense mutations in early-onset familial Alzheimer's disease
Sherrington, R., Rogaev, E. I., Liang, Y., Rogaeva, E. A., Levesque, G., Ikeda, M., Chi, H., Lin, C., Li, G., Holman, K., et al. (1995) · Nature · doi:10.1038/375754a0 · Cited by 4,051
Emerging roles of caspase-3 in apoptosis
Porter, A. G., & Jänicke, R. U. (1999) · Cell Death and Differentiation · doi:10.1038/sj.cdd.4400476 · Cited by 3,921
Organogenesis in a dish: Modeling development and disease using organoid technologies
Lancaster, M. A., & Knoblich, J. A. (2014) · Science · doi:10.1126/science.1247125 · Cited by 2,931
Brain-Region-Specific Organoids Using Mini-bioreactors for Modeling ZIKV Exposure
Qian, X., Nguyen, H. N., Song, M. M., Hadiono, C., Ogden, S. C., Hammack, C., Yao, B., Hamersky, G. R., Jacob, F., Zhong, C., et al. (2016) · Cell · doi:10.1016/j.cell.2016.04.032 · Cited by 2,127
Generation of cerebral organoids from human pluripotent stem cells
Lancaster, M. A., & Knoblich, J. A. (2014) · Nature Protocols · doi:10.1038/nprot.2014.158 · Cited by 1,823
Probing sporadic and familial Alzheimer’s disease using induced pluripotent stem cells
Israel, M. A., Yuan, S. H., Bardy, C., Reyna, S. M., Mu, Y., Herrera, C., Hefferan, M. P., Van Gorp, S., Nazor, K. L., Boscolo, F. S., et al. (2012) · Nature · doi:10.1038/nature10821 · Cited by 1,253
A three-dimensional human neural cell culture model of Alzheimer’s disease
Choi, S. H., Kim, Y. H., Hebisch, M., Sliwinski, C., Lee, S., D’Avanzo, C., Chen, H., Hooli, B., Asselin, C., Muffat, J., et al. (2014) · Nature · doi:10.1038/nature13800 · Cited by 1,195

Show all 33 references

Sorted by citations

Items 201–300 of 432

Stem cell-based 3D brain organoids for mimicking, investigating, and challenging Alzheimer's diseases
Cordella, F., Brighi, C., Soloperto, A., & Angelantonio, S. D. (2021) · Neural Regeneration Research · doi:10.4103/1673-5374.317976 · Cited by 14
A 3D human iPSC-derived multi-cell type neurosphere system to model cellular responses to chronic amyloidosis
Wendt, S., Lin, A. J., Ebert, S. N., Brennan, D. J., Cai, W., Bai, Y., Kong, D. Y., Sorrentino, S., Groten, C. J., Lee, C., et al. (2025) · Journal of Neuroinflammation · doi:10.1186/s12974-025-03433-3 · Cited by 13
Inflammatory Response and Exosome Biogenesis of Choroid Plexus Organoids Derived from Human Pluripotent Stem Cells
Muok, L., Liu, C., Chen, X., Esmonde, C., Arthur, P., Wang, X. S., Singh, M. S., Driscoll, T. P., & Li, Y. (2023) · International Journal of Molecular Sciences · doi:10.3390/ijms24087660 · Cited by 13
Efficient generation of human cerebral organoids directly from adherent cultures of pluripotent stem cells
González-Sastre, R., Coronel, R., Bernabeu-Zornoza, A., Mateos-Martínez, P., Rosca, A., López-Alonso, V., & Liste, I. (2024) · Journal of Tissue Engineering · doi:10.1177/20417314231226027 · Cited by 13
Biomimetic design and integrated biofabrication of an in-vitro three-dimensional multi-scale multilayer cortical model
Wang, L., Bai, L., Wang, S., Zhou, J., Liu, Y., Zhang, C., Yao, S., He, J., Liu, C., & Li, D. (2024) · Materials Today Bio · doi:10.1016/j.mtbio.2024.101176 · Cited by 13
A stroke organoids-multiomics platform to study injury mechanism and drug response
Zhu, W., Wu, Y., Li, X., Yang, H., He, F., Ma, J., Wei, J., & Leng, L. (2024) · Bioactive Materials · doi:10.1016/j.bioactmat.2024.09.038 · Cited by 14
Modeling the Blood–Brain Barrier to Understand Drug Delivery in Alzheimer’s Disease
Wasielewska, J. M., Chaves, J., White, A. R., & Oikari, L. E. (2020) · Exon Publications eBooks · doi:10.36255/exonpublications.alzheimersdisease.2020.ch7 · Cited by 13
Protocol for controlled cortical impact in human cerebral organoids to model traumatic brain injury
Ramírez, S., Mukherjee, A., Sepulveda, S. E., Gherardelli, C., Becerra-Calixto, A., Bravo‐Vasquez, N., & Soto, C. A. (2021) · STAR Protocols · doi:10.1016/j.xpro.2021.100987 · Cited by 13
Self-citation
From neurodevelopment to neurodegeneration: utilizing human stem cell models to gain insight into Down syndrome
Watson, L. A., & Meharena, H. S. (2023) · Frontiers in Genetics · doi:10.3389/fgene.2023.1198129 · Cited by 13
Modeling neurodegenerative diseases with brain organoids: from development to disease applications
Larriba-González, T., García-Martín, M., Ojeda-Hernández, D. D., Rincón-Cerrada, P., Martín-Blanco, L., Benito‐Martín, M. S., Selma-Calvo, B., de la Fuente-Martín, S., Matías‐Guiu, J. A., Matías‐Guiu, J., et al. (2025) · Frontiers in Cell and Developmental Biology · doi:10.3389/fcell.2025.1663286 · Cited by 12
Therapeutic Transplantation of Human Central Nervous System Organoids for Neural Reconstruction
Hong, S. J., Bock, M., Zhang, S., An, S. B., & Han, I. (2024) · International Journal of Molecular Sciences · doi:10.3390/ijms25158540 · Cited by 12
GRAMD1B is a regulator of lipid homeostasis, autophagic flux and phosphorylated tau
Acosta Ingram, D., Turkes, E., Kim, T. Y., Vo, S., Sweeney, N., Bonte, M.-A., Rutherford, R., Julian, D. L., Pan, M., Marsh, J., et al. (2025) · Nature Communications · doi:10.1038/s41467-025-58585-w · Cited by 12
Mass spectrometry analysis of tau and amyloid‐beta in iPSC‐derived models of Alzheimer’s disease and dementia
Arber, C., Alatza, A., Leckey, C. A., Paterson, R. W., Zetterberg, H., & Wray, S. (2021) · Journal of Neurochemistry · doi:10.1111/jnc.15315 · Cited by 13
Advances in construction and modeling of functional neural circuits in vitro
Chow, S. Y. A., Hu, H., Osaki, T., Levi, T., & Ikeuchi, Y. (2022) · Neurochemical Research · doi:10.1007/s11064-022-03682-1 · Cited by 12
Alzheimer’s disease like neuropathology in Down syndrome cortical organoids
Zhao, H., & Haddad, G. G. (2022) · Frontiers in Cellular Neuroscience · doi:10.3389/fncel.2022.1050432 · Cited by 12
Brain organoids as precision models for neurodegenerative diseases: from disease modeling to drug discovery
Zheng, Y., Zhou, W., Chang, H., & Zheng, K. (2026) · Frontiers in Neuroscience · doi:10.3389/fnins.2026.1764964 · Cited by 12
Urine-derived cells from the aged donor for the 2D/3D modeling of neural cells via iPSCs
Supakul, S., Hatakeyama, Y., Leventoux, N., Itsuno, M., Numata, N., Hiramine, H., Morimoto, S., Iwata, A., Maeda, S., & Okano, H. (2023) · Aging Brain · doi:10.1016/j.nbas.2023.100101 · Cited by 11
Immunosenescence and organoids: pathophysiology and therapeutic opportunities
Kamroo, A., Kakroudi, M. H., Sarmadian, A. J., Firouzabadi, A., Mousavi, S., Yazdanpanah, N., Saleki, K., & Rezaei, N. (2025) · Immunity & Ageing · doi:10.1186/s12979-025-00530-y · Cited by 12
Human-Derived Brain Models: Windows into Neuropsychiatric Disorders and Drug Therapies
Papariello, A. R., & Litwa, K. (2019) · Assay and Drug Development Technologies · doi:10.1089/adt.2019.922 · Cited by 11
Phenotyping Neurodegeneration in Human iPSCs
Li, J., & Fraenkel, E. (2021) · Annual Review of Biomedical Data Science · doi:10.1146/annurev-biodatasci-092820-025214 · Cited by 11
Engineering Organoids for in vitro Modeling of Phenylketonuria
Borges, A. C., Broersen, K., Leandro, P., & Fernandes, T. G. (2022) · Frontiers in Molecular Neuroscience · doi:10.3389/fnmol.2021.787242 · Cited by 14
Nutritional assessment models for Alzheimer's disease: Advances and perspectives
Wen, Y., Zhang, L., Li, N., Tong, A., & Zhao, C. (2023) · Food Frontiers · doi:10.1002/fft2.216 · Cited by 11
Neural lineage differentiation of human pluripotent stem cells: Advances in disease modeling
Yan, Y.-W., Qian, E. S., Woodard, L. E., & Bejoy, J. (2023) · World Journal of Stem Cells · doi:10.4252/wjsc.v15.i6.530 · Cited by 11
Advances and Challenges in Cerebral Organoids Research
Luo, D., Xu, J., Liu, F., & Gu, Z. (2024) · Advanced NanoBiomed Research · doi:10.1002/anbr.202300126 · Cited by 11
Suppressing neuroinflammation using the near-infrared light emitted by (Sr,Ba)Ga12O19: Cr3+ phosphor
Liu, Q., Yu, F., Chamkouri, H., Guo, Y., Chen, P., Wang, B., Liu, D., & Chen, L. (2024) · Advanced Photonics Nexus · doi:10.1117/1.apn.3.3.036008 · Cited by 10
Advances in Recapitulating Alzheimer’s Disease Phenotypes Using Human Induced Pluripotent Stem Cell-Based In Vitro Models
Hasan, M. F., & Trushina, E. V. (2022) · Brain Sciences · doi:10.3390/brainsci12050552 · Cited by 10
Building in vitro models of the brain to understand the role ofAPOEin Alzheimer’s disease
Pinals, R. L., & Tsai, L. (2022) · Life Science Alliance · doi:10.26508/lsa.202201542 · Cited by 10
Towards a Mechanistic Model of Tau-Mediated Pathology in Tauopathies: What Can We Learn from Cell-Based In Vitro Assays?
Sala‐Jarque, J., Zimkowska, K., Ávila, J., Ferrer, I., & del Río, J. A. (2022) · International Journal of Molecular Sciences · doi:10.3390/ijms231911527 · Cited by 11
Human-Induced Pluripotent Stem Cell (hiPSC)-Derived Neurons and Glia for the Elucidation of Pathogenic Mechanisms in Alzheimer’s Disease
Young, J. E., & Goldstein, L. S. B. (2022) · Methods in molecular biology · doi:10.1007/978-1-0716-2655-9_6 · Cited by 10
Unprecedented Potential for Neural Drug Discovery Based on Self-Organizing hiPSC Platforms
Cota-Coronado, A., Durnall, J. C., Díaz, N. F., Thompson, L. H., & Díaz-Martínez, N. E. (2020) · Molecules · doi:10.3390/molecules25051150 · Cited by 10
Unraveling pathological mechanisms in neurological disorders: the impact of cell-based and organoid models
Langlie, J. A., Mittal, R., Finberg, A., Bencie, N. B., Mittal, J., Omidian, H., Omidi, Y., & Eshraghi, A. A. (2022) · Neural Regeneration Research · doi:10.4103/1673-5374.335836 · Cited by 10
Understanding monocyte-driven neuroinflammation in Alzheimer’s disease using human cortical organoid microphysiological systems
Tian, C., Ao, Z., Cerneckis, J., Cai, H., Chen, L., Niu, H., Takayama, K., Kim, J., Shi, Y., Gu, M., et al. (2025) · Science Advances · doi:10.1126/sciadv.adu2708 · Cited by 9
Emerging brain organoids: 3D models to decipher, identify and revolutionize brain
Zhao, Y., Wang, T., Liu, J., Wang, Z., & Lu, Y. (2025) · Bioactive Materials · doi:10.1016/j.bioactmat.2025.01.025 · Cited by 9
Abnormal synaptic architecture in iPSC-derived neurons from a multi-generational family with genetic Creutzfeldt-Jakob disease
Gojanovich, A. D., Le, N. T. T., Mercer, R. C. C., Park, S., Wu, B., Anane, A., Vultaggio, J. S., Mostoslavsky, G., & Harris, D. A. (2024) · Stem Cell Reports · doi:10.1016/j.stemcr.2024.08.010 · Cited by 9
Great Expectations: Induced pluripotent stem cell technologies in neurodevelopmental impairments
Zhang, X., Li, Z., Liu, Y., & Gai, Z. (2020) · International Journal of Medical Sciences · doi:10.7150/ijms.51842 · Cited by 10
Patient-derived iPSCs, a reliable in vitro model for the investigation of Alzheimer’s disease
Asiamah, E. A., Guo, R., Kong, D., Feng, B., He, J., Zhang, W., Liu, X., Du, X., Ma, Z., Liu, B., et al. (2021) · Reviews in the Neurosciences · doi:10.1515/revneuro-2020-0065 · Cited by 9
Neurodegenerative brain models vs. cell replacement or restoration therapy: A review on promises and pitfalls
Kumar, D., & Hassan, M. I. (2021) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2021.11.040 · Cited by 9
Extracellular Vesicle Treatment Alleviates Neurodevelopmental and Neurodegenerative Pathology in Cortical Spheroid Model of Down Syndrome
Campbell, N. B., Patel, Y., Moore, T. L., Medalla, M., & Zeldich, E. (2023) · International Journal of Molecular Sciences · doi:10.3390/ijms24043477 · Cited by 8
Monitoring the Dynamics of Alzheimer's Disease Biomarkers and the APOE –Tau Axis via Human Cerebral Organoids with Immuno‐SERS
Jo, Y., Kim, Y. J., Kang, R., Lee, S., Nguyen, D. D., Park, S., Lee, D., Han, J. W., Mook‐Jung, I., Lee, L. P., et al. (2025) · Advanced Science · doi:10.1002/advs.202505660 · Cited by 8
Human iPSC-derived cerebral organoids reveal oxytocin-mediated protection against amyloid-β pathology
Asaba, T., Hamano, S., Nanmo, A., Seo, J., Kageyama, T., & Fukuda, J. (2025) · Regenerative Therapy · doi:10.1016/j.reth.2025.06.013 · Cited by 8
Advanced neural activity mapping in brain organoids via field potential imaging with ultra-high-density CMOS microelectrode arrays
Yokoi, R., Matsuda, N., Ishibashi, Y., & Suzuki, I. (2025) · Frontiers in Neuroscience · doi:10.3389/fnins.2025.1634582 · Cited by 11
A neuroimmune cerebral assembloid model to study the pathophysiology of familial Alzheimer’s disease
Becerra-Calixto, A., Banerjee, A., Fan, H., Tan, C., Lee, E., McCullough, L. D., & Lee, J. (2025) · Journal of Neuroinflammation · doi:10.1186/s12974-025-03544-x · Cited by 10
Self-citation
Autophagy Activators Normalize Aberrant Tau Proteostasis and Rescue Synapses in Human Familial Alzheimer's Disease iPSC‐Derived Cortical Organoids
Labra, S. R., Compher, J., Prabhavalkar, A., Almaraz, M., Kwong, C. C., Baal, C., Talantova, M. V., Dolatabadi, N., Piña‐Sanz, J., Wang, Y., et al. (2026) · Advanced Science · doi:10.1002/advs.202514783 · Cited by 9
Amyloid beta accumulations and enhanced neuronal differentiation in cerebral organoids of Dutch-type cerebral amyloid angiopathy patients
Daoutsali, E., Pepers, B. A., Stamatakis, S., van der Graaf, L. M., Terwindt, G. M., Parfitt, D. A., Buijsen, R. A. M., & van Roon‐Mom, W. (2023) · Frontiers in Aging Neuroscience · doi:10.3389/fnagi.2022.1048584 · Cited by 8
Robust Tissue Fabrication for Long-Term Culture of iPSC-Derived Brain Organoids for Aging Research
Koch, L. S., Buentello, D. C., & Broersen, K. (2023) · Journal of Visualized Experiments · doi:10.3791/64586 · Cited by 8
GABAergic signaling abnormalities in a novel CLU mutation Alzheimer's disease mouse model
Chen, C., Tang, X., Lan, Z., Wan, C., Su, H., Li, W., Li, Y., Zhou, X. W., Gao, H., Feng, X., et al. (2023) · Translational research · doi:10.1016/j.trsl.2023.05.003 · Cited by 8
A narrative review of organoids for investigating organ aging: opportunities and challenges
Sun, X., Sun, F., Zhang, Y., Qu, J. T., Zhang, W., & Liu, G. (2023) · Journal of Bio-X Research · doi:10.1097/jbr.0000000000000139 · Cited by 8
Advances in iPSC Technology in Neural Disease Modeling, Drug Screening, and Therapy
Dai, S., Qiu, L., Veeraraghavan, V. P., Sheu, C., & Mony, U. (2023) · Current Stem Cell Research & Therapy · doi:10.2174/1574888x18666230608105703 · Cited by 8
Modeling SARS-CoV-2 infection in individuals with opioid use disorder with brain organoids
Willner, M. J., Xiao, Y., Kim, H. S., Chen, X., Xu, B., & Leong, K. W. (2021) · Journal of Tissue Engineering · doi:10.1177/2041731420985299 · Cited by 8
Dynamic neuroinflammatory profiles predict Alzheimer’s disease pathology in microglia-containing cerebral organoidsPreprint
Kuhn, M. K., Kang, R. Y., Kim, C., Tagay, Y., Morris, N., Tabdanov, E. D., Elcheva, I. A., & Proctor, E. A. (2023) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2023.11.16.567220 · Cited by 9
Mouse brain organoids model in vivo neurodevelopment and function and capture differences to humanPreprint
Sanchez, D. J. L.-D., Lindhout, F. W., Anderson, A. J., Pellegrini, L., & Lancaster, M. A. (2024) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2024.12.21.629881 · Cited by 8
Organoids Modeling Stroke in a Petri Dish
Giorgi, C., Castelli, V., d’Angelo, M., & Cimini, A. (2024) · Biomedicines · doi:10.3390/biomedicines12040877 · Cited by 7
Biomanufacturing and Curcumin-Loading of Human Choroid Plexus Organoid-Derived Extracellular Vesicles from a Vertical-Wheel Bioreactor to Alleviate Neuro-Inflammation
Ene, J., Muok, L., Gonzalez, V., Sanchez, N., Nathani, A., Syed, F., Liu, Z. L., Singh, M., Driscoll, T. P., & Li, Y. (2025) · Biomedicines · doi:10.3390/biomedicines13051069 · Cited by 7
Brain Organoids in Neurodegenerative Disease Modeling: Advances, Applications and Future Perspectives
Wang, W., Tan, S., Zuo, X., Gao, X., Ma, L., Sun, R., Liang, G., Yin, L., Pu, Y., & Zhang, J. (2025) · Molecular Neurobiology · doi:10.1007/s12035-025-05293-7 · Cited by 7
Brain Organoids as Emerging Platforms for Modeling Neurodegenerative Diseases: Progress, Challenges, and Future Directions
Kaya, Y., & Kırboğa, K. K. (2026) · Journal of Neurochemistry · doi:10.1111/jnc.70395 · Cited by 7
Bridging population and cell: modelling complex diseases with human induced pluripotent stem cells
van Zanten, E., Loehrer, E. A., van Meurs, J. B. J., Narcisi, R., Gribnau, J. H., Poot, R. A., & Adams, H. H. H. (2026) · European Journal of Human Genetics · doi:10.1038/s41431-026-02071-4 · Cited by 7
Geometrically engineered organoid units and their assembly for pre-construction of organ structures
Kadotani, A., Hayase, G., & Yoshino, D. (2024) · APL Bioengineering · doi:10.1063/5.0222866 · Cited by 7
N-Acetylcysteine prevents amyloid-β secretion in neurons derived from human pluripotent stem cells with trisomy 21
Toshikawa, H., Ikenaka, A., Li, L., Nishinaka‐Arai, Y., Niwa, A., Ashida, A., Kazuki, Y., Nakahata, T., Tamai, H., Russell, D. W., et al. (2021) · Scientific Reports · doi:10.1038/s41598-021-96697-7 · Cited by 7
Towards elucidating disease-relevant states of neurons and glia by CRISPR-based functional genomics
Leng, K., & Kampmann, M. (2022) · Genome Medicine · doi:10.1186/s13073-022-01134-7 · Cited by 7
Three-dimensional human brain organoids: A next-generation model to decode Alzheimer’s disease
Kumari, S., Ashvin, Dhapola, R., Sharma, P., Paidlewar, M., Vellingiri, B., Medhi, B., & HariKrishnaReddy, D. (2025) · Ageing Research Reviews · doi:10.1016/j.arr.2025.102928 · Cited by 6
A human iPSC model of tauopathies engineered for 4R tau isoform expression endogenously develops late-stage neuronal tau pathology
Dannert, A., Schulz, N., Klimmt, J., Knez, L., Groschup, B., Gonçalves, C. C., Carraro, C., Schifferer, M., Brendel, M., & Paquet, D. (2026) · Science Translational Medicine · doi:10.1126/scitranslmed.adu9845 · Cited by 6
Understanding the neurological implications of acute and long COVID using brain organoids
García‐González, L., Martí-Sarrias, A., Puertas, M. C., Bayón-Gil, Á., Resa‐Infante, P., Martínez‐Picado, J., Navarro, A., & Acosta, S. (2023) · Disease Models & Mechanisms · doi:10.1242/dmm.050049 · Cited by 7
In and out: Benchmarking in vitro, in vivo, ex vivo, and xenografting approaches for an integrative brain disease modeling pipeline
Pereira, M. F., Shyti, R., & Testa, G. (2024) · Stem Cell Reports · doi:10.1016/j.stemcr.2024.05.004 · Cited by 8
Towards More Human and Humane Testing: The Role of the Device Supplier Industry
Cuanalo-Contreras, K., & Benkmann, D. (2022) · Alternatives to Laboratory Animals · doi:10.1177/02611929211073132 · Cited by 6
A Comprehensive Update of Cerebral Organoids between Applications and Challenges
Li, X., Shopit, A., & Wang, J. (2022) · Oxidative Medicine and Cellular Longevity · doi:10.1155/2022/7264649 · Cited by 6
A Political and ethical landscape of brain organoid research
Schneider, E., Samsa, L. A., & Dubljević, V. (2023) · Molecular Psychology Brain Behavior and Society · doi:10.12688/molpsychol.17521.1 · Cited by 6
iPSC-derived neural organoids in dementia research: Recent advances and future directions
Shima, S., Kondo, T., & Inoue, H. (2025) · Neuroscience Research · doi:10.1016/j.neures.2025.104980 · Cited by 6
Neuronal Organoid Engineering and Disease-Focused High-Throughput Neuropharmacology: Advances, Limitations, and Translational Strategies
Far, B. F., Omidvar, K., Heidari, E., Ebrahimi, M., Mohammadi, Y., & Ertaş, Y. N. (2025) · ACS Pharmacology & Translational Science · doi:10.1021/acsptsci.5c00407 · Cited by 5
Recent advances and applications of human brain models
Nishimura, K., Osaki, H., Tezuka, K., Nakashima, D., Numata, S., & Masamizu, Y. (2024) · Frontiers in Neural Circuits · doi:10.3389/fncir.2024.1453958 · Cited by 5
Microglial Drivers of Alzheimer's Disease Pathology: An Evolution of Diverse Participating States
Kuhn, M. K., & Proctor, E. A. (2024) · Proteins Structure Function and Bioinformatics · doi:10.1002/prot.26723 · Cited by 5
Sea Squirt-Derived Peptide WLP Mitigates OKA-Induced Alzheimer’s Disease-like Phenotypes in Human Cerebral Organoid
Chen, Q., Wang, Z., Guo, W., Xue, A., Bian, G., Guo, X., Lu, S., Zeng, P., Li, H., Zhu, X., et al. (2025) · Antioxidants · doi:10.3390/antiox14050553 · Cited by 5
Human cerebral organoids: Complex, versatile, and human-relevant models of neural development and brain diseases
Coronel, R., González-Sastre, R., Mateos-Martínez, P., Maeso, L., Llorente-Beneyto, E., Martín-Benito, S., Gagosian, V. S. C., Foti, L., González, M. D. C., López-Alonso, V., et al. (2025) · Neural Regeneration Research · doi:10.4103/nrr.nrr-d-24-01639 · Cited by 5
Bibliometric analysis of cerebral organoids and diseases in the last 10 years
Luo, B., Liu, K., & Fan, J. (2023) · Ibrain · doi:10.1002/ibra.12139 · Cited by 5
Stem cell-derived three-dimensional (organoid) models of Alzheimer’s disease: a precision medicine approach
Mahairaki, V., Kim, S. Y., & Li, J. (2021) · Neural Regeneration Research · doi:10.4103/1673-5374.303019 · Cited by 5
Opportunities and Challenges in Precision Neurotherapeutics
Mujica-Parodi, L. R., Öngür, D., & Richardson, R. M. (2026) · Annual Review of Biomedical Engineering · doi:10.1146/annurev-bioeng-110824-031709 · Cited by 4
Metabolic Assessment in Human Pluripotent Stem Cell‐Derived Cerebral Organoids Using HR‐MAS NMR Spectroscopy
Bolaños, M. A. C., Chinchalongporn, V., Biswas, R. G., Bailey, C., Wu, M., Soong, R., Saleh, F., Simpson, A. J., Schuurmans, C. J., & Near, J. (2026) · NMR in Biomedicine · doi:10.1002/nbm.70343 · Cited by 4
Apigenin: Exploring its neuroprotective potential in neurodegenerative disorders: Mechanisms and promising therapeutic applications
Bhratee, A., Anand, P., & Singh, S. (2023) · Pharmaspire · doi:10.56933/pharmaspire.2023.15138 · Cited by 4
Disease phenotypic screening in neuron-glia cocultures identifies blockers of inflammatory neurodegeneration
Birkle, T. J. Y., Willems, H. M. G., Skidmore, J., & Brown, G. C. (2024) · iScience · doi:10.1016/j.isci.2024.109454 · Cited by 4
Modeling Alzheimer's disease using cerebral organoids: Current challenges and prospects
Abijo, A. Z., Olatunji, S., Adelodun, S. T., Asamu, M. O., & Omeiza, N. A. (2024) · Brain Organoid and Systems Neuroscience Journal · doi:10.1016/j.bosn.2024.09.001 · Cited by 6
Human induced pluripotent stem cell– based modeling of Alzheimer’s disease, a glial perspective
Chen, X., Yang, Q., Liu, Z., & Shi, Y. (2021) · Elsevier eBooks · doi:10.1016/b978-0-323-85764-2.00006-5 · Cited by 4
Cerebral Organoid Arrays for Batch Phenotypic Analysis in Sections and Three Dimensions
Chen, J., Ma, H., Deng, Z., Luo, Q., Gong, H., Long, B., & Li, X. (2023) · International Journal of Molecular Sciences · doi:10.3390/ijms241813903 · Cited by 3
What makes the human brain special: from cellular function to clinical translation
van Loo, K. M. J., Bak, A., Hodge, R. D., Bedogni, F., Ramirez, J. S. B., Emerson, S., Höllig, A., Mansvelder, H. D., Goriounova, N. A., Ramirez, J., et al. (2025) · Journal of Neurophysiology · doi:10.1152/jn.00120.2025 · Cited by 4
Unconventional Non-rodent Models in Neurological Research: Exploring New Paths to Translational Insights
Devi, A., Saini, C. S., Shiven, A., Kumar, A., Sharma, A., Joshi, N., Kour, S., Khan, Z. A., Dewangan, H. K., & Dwibedi, V. (2025) · Journal of Molecular Neuroscience · doi:10.1007/s12031-025-02408-6 · Cited by 3
Non-invasive quantitative acoustic imaging of intact brain organoids for structural assessment in drug discovery
Habib, A., Mateos, P. C., Dubey, V. K., Ahmad, A., Acharya, G., Ahluwalia, B. S., Varshney, M., & Melandsø, F. (2025) · Materials Today Advances · doi:10.1016/j.mtadv.2025.100651 · Cited by 3
Pluripotent stem cells-based neural organoids for modelling human brain development and diseases
Tong, L., Tian, P., Wang, R., Niu, S., Wang, R., Ye, Y., Wu, Y., Zhang, W., Wang, Y., Foggetti, A., et al. (2025) · Cell & Bioscience · doi:10.1186/s13578-025-01515-6 · Cited by 3
Advances in the pathophysiological study of brain development: application of cerebral organoid combined with Spatial omics technology
Wang, J., Sun, Z., Zhou, Y., Wang, L., & Liu, J. (2026) · Stem Cell Research & Therapy · doi:10.1186/s13287-025-04885-3 · Cited by 3
Patient-derived brain organoids reveal divergent neuronal activity across subpopulations of autism spectrum disorder
Perets, N., Kerem, L., Waiskopf, N., Horesh, N., Goldman, I., Avichzer, J., Bril, D., Tobelaim, W., Barashi, M., David, L., et al. (2026) · Translational Psychiatry · doi:10.1038/s41398-026-03890-1 · Cited by 3
Advanced 3D Platforms for Modeling CNS Neuroinflammation: Cell Integration, Techniques, and Challenges
Aiyegbusi, E. D., Stefano, J. D., & Dooley, D. (2026) · Advanced Healthcare Materials · doi:10.1002/adhm.202504508 · Cited by 3
Current Update on Promising New Anti-Alzheimer’s Drugs in Different Phases of Clinical Development: Where Exactly Are We Lacking?
Sharma, P. K., Yadav, M., & Mehta, N. (2024) · Journal of the Association of Physicians of India · doi:10.59556/japi.72.0755 · Cited by 3
Brain organoids and genome editing: A new era in understanding human brain development and disorders
Zhou, M., Cao, Y., Yue, K., Wu, W., Xie, Y., Hu, D., Zhao, J., Xu, F., Guo, J., Li, Z., et al. (2025) · Neural Regeneration Research · doi:10.4103/nrr.nrr-d-24-01546 · Cited by 3
Helicobacter pylori infection impairs glucose homeostasis through gut microbiota dysbiosis
Chen, H., Wang, Z., Su, W., Li, S., Ye, Q., Zhang, G., & Zhou, X. (2025) · BMC Microbiology · doi:10.1186/s12866-025-04402-9 · Cited by 3
Deepening the understanding of CNVs on chromosome 15q11–13 by using hiPSCs: An overview
Giovenale, A. M. G., Ruotolo, G., Soriano, A. A., Turco, E. M., Rotundo, G., Casamassa, A., D’Anzi, A., Vescovi, A. L., & Rosati, J. D. (2023) · Frontiers in Cell and Developmental Biology · doi:10.3389/fcell.2022.1107881 · Cited by 3
Applications of Induced Pluripotent Stem Cell-Derived Glia in Brain Disease Research and Treatment
Yang, Z., Gong, M., Yang, C., Chen, C., & Zhang, K. (2023) · Handbook of experimental pharmacology · doi:10.1007/164_2023_697 · Cited by 3
Prion propagation and cellular dysfunction in prion disease: Disconnecting the dots
Foliaki, S. T., & Haigh, C. L. (2023) · PLoS Pathogens · doi:10.1371/journal.ppat.1011714 · Cited by 3
Advances in the Differentiation of hiPSCs into Cerebellar Neuronal Cells
Wang, Y., Liu, W., Jiao, Y., Yang, Y., Shan, D., Ji, X., Zhang, R., Zhan, Z., Tang, Y., Guo, D., et al. (2024) · Stem Cell Reviews and Reports · doi:10.1007/s12015-024-10763-x · Cited by 3
In Vitro Cell Line Culture for Brain Research and Its Limitations
Dabhekar, S. V., Dhokne, M. D., Dalal, V., Lokhande, S., Taksande, B. G., Nakhate, K. T., Umekar, M. J., & Mangrulkar, S. V. (2024) · Springer eBooks · doi:10.1007/978-981-97-2859-6_11 · Cited by 3
An in vitro workflow of neuron-laden agarose-laminin hydrogel for studying small molecule-induced amyloidogenic condition
Namchaiw, P., Bunreangsri, P., Eiamcharoen, P., Eiamboonsert, S., & Poo-arporn, R. P. (2022) · PLoS ONE · doi:10.1371/journal.pone.0273458 · Cited by 3
Engineered Cerebral Organoids Recapitulate Adult Tau Expression and Disease-relevant Changes in Tau SplicingPreprint
Lovejoy, C. E. J., Alatza, A., Arber, C., Galasso, G., Bradshaw, T. Y., Verheyen, A., Lashley, T., Révész, T., Hardy, J., Karch, C. M. M., et al. (2020) · doi:10.21203/rs.3.rs-37620/v1 · Cited by 3
All-in-one generation and multiomic profiling of human whole brain organoid on a millifluidic plate
Zhao, W., Wang, Y., Chen, T., Shen, M., Wang, J., Huang, X., Zhu, L., Yu, T., Zhang, Z., Yang, Y., et al. (2025) · Materials Today Bio · doi:10.1016/j.mtbio.2025.102653 · Cited by 2
Establishment of a human inner ear model reveals that gentamicin C2b is substantially less ototoxic than clinical gentamicin
Jeong, M., Mohri, H., Han, D., Sakagami, M., & Stanković, K. M. (2026) · Biomedicine & Pharmacotherapy · doi:10.1016/j.biopha.2025.118915 · Cited by 2

Authors: Gonzalez, C., Armijo, E., Bravo-Alegria, J., Becerra-Calixto, A., Mays, C. E., & Soto, C.
Affiliations: Universidad de Los Andes, Chile (CL)
Universidad de Antioquia (CO)
The University of Texas Health Science Center at Houston (US)
Corresponding author: Soto, Claudio
ORCID: Bravo-Alegria, Javiera
Becerra-Calixto, Andrea
Soto, Claudio
Venue: Molecular Psychiatry
Publisher: Springer Science and Business Media LLC
Published: 2018-08-31
Volume: 23
Issue: 12
Pages: 2363-2374
ISSN: 1359-4184 (ISSN-L)
1476-5578
DOI: 10.1038/s41380-018-0229-8
PMC: PMC6594704
OpenAlex: W2889449128
Cited by: 430
References: 33
Citations by year: 2026: 43 · 2025: 55 · 2024: 63 · 2023: 60 · 2022: 56 · 2021: 78 · 2020: 48 · 2019: 24 · 2018: 2
FWCI: 26.30
Type: journal article
Language: en
Open access: green
www.ncbi.nlm.nih.gov
Licence: www.springer.com/tdm (tdm)
Locations: Molecular Psychiatry (publishedVersion)
PubMed (publishedVersion)
PubMed Central (acceptedVersion)
Funders: National Institute on Aging (R01AG061069)
Topics: Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Alzheimer's disease research and treatments
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Pluripotent Stem Cells Research
Health Sciences › Medicine › Psychiatry and Mental health › Dementia and Cognitive Impairment Research
Keywords: Alzheimer's disease, brain organoids, amyloid plaques, neurofibrillary tangles, tau pathology, amyloid-beta, induced pluripotent stem cells, familial Alzheimer's disease, Down syndrome, patient-specific disease modeling, drug screening
MeSH terms: Aged, Alzheimer Disease, Brain, Cerebral Cortex, Female, Humans, Infant, Male, Middle Aged, Neurons, Organoids, Phosphorylation, Amyloid beta-Peptides, Neurofibrillary Tangles, tau Proteins, Cell Culture Techniques, Induced Pluripotent Stem Cells, Plaque, Amyloid

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

Modeling amyloid beta and tau pathology in human cerebral organoids

Abstract

Overview

Integrity signals

Verification

Claims the paper makes

Claims other papers make about this one

Self-check

Citations

Cites

Cited by

Related

Metadata

Record

About these checks