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Cited by 26Hair Growth and DisordersPluripotent Stem Cells ResearchCongenital heart defects research

Direct reprogramming of epidermal cells toward sweat gland-like cells by defined factors

Open access
Yao, Bin, Xie, Jiangfan, Liu, Nanbo, Hu, Tian, Song, Wei, Huang, Sha, Fu, Xiaobing·2019·Cell Death & Disease
DOI 10.1038/s41419-019-1503-7PMC PMC6426881
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Abstract

Several studies have reported inducing adult cells into sweat gland-like cells; however, slow transition and low efficiency limit the potential for cell-based treatment. Here, we show that overexpression of the transcription factor FoxC1 was sufficient to reprogram epidermal cells to induced functional sweat gland-like cells (iSGCs). The iSGCs expressing secreting-related genes, had a global gene expression profile between fetal SGCs (P5) and adult SGCs (P28). Moreover, iSGCs transplanted into the burn mice model facilitated wound repair and sweat gland regeneration. We further demonstrated that the Foxc1 upregulated BMP5 transcription and BMP5 is responsible for the cell-type transition. Collectively, this study shows that lineage reprogramming of epidermal cells into iSGCs provides an excellent cell source and a promising regenerative strategy for anhidrosis and hypohidrosis.

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  • Recent studies have demonstrated the potential conversion of autologous somatic cells, such as epidermal progenitors or mesenchymal stem cells, into SwG lineages through reprogramming manipulations.

    “Recent studies have demonstrated the potential conversion of autologous somatic cells, such as the epidermal progenitors[ 3 ] or mesenchymal stem cells,[ 4 ] into SwG lineages through reprogramming manipulations.”

    From Sweat Gland Organoids Originating from Reprogrammed Epidermal Keratinocytes Functionally Recapitulated Damaged Skin (2021) · cites this paper as [3]

    The Abstract reports that FoxC1 overexpression reprogrammed epidermal cells into functional sweat gland-like cells. The Introduction says epidermal cells develop from epidermal progenitors, but the Methods describe isolating epidermal cells—not progenitors—and the paper does not establish that the transplanted cells were autologous. The mesenchymal-stem-cell example has a separate citation and is not assessed here. (LLM, conf=0.86)

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Research works printed but not linked: 31

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Cited by

Sorted by citations

Authors
Yao, B., Xie, J., Liu, N., Hu, T., Song, W., Huang, S., & Fu, X.
Affiliations
  • Chinese PLA General Hospital (CN)
  • Guangdong Provincial People's Hospital (CN)
  • Nankai University (CN)
Corresponding author
  • Huang, Sha
  • Fu, Xiaobing
ORCID
Venue
Cell Death & Disease
Publisher
Springer Science and Business Media LLC
Published
2019-03-20
Volume
10
Issue
4
Pages
272-272
ISSN
2041-4889 (ISSN-L)
DOI
10.1038/s41419-019-1503-7
PMC
PMC6426881
OpenAlex
W2925340838
Cited by
26
References
4
Citations by year
2026: 4 · 2025: 5 · 2024: 5 · 2023: 2 · 2022: 2 · 2021: 6 · 2020: 1 · 2019: 1
FWCI
3.20
Type
journal article
Language
en
Open access
Licence
Locations
  • Cell Death and Disease (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion, other-oa)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
Funders
  • National Natural Science Foundation of China (81830064, AWS17J005, 81571909, 2017YFC1103300, 81721092, 81701906, 2017-JQPY-002)
  • General Hospital of People’s Liberation Army
Topics
  • Health Sciences › Medicine › Urology › Hair Growth and Disorders
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Pluripotent Stem Cells Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Congenital heart defects research
Keywords
FOXC1, BMP5, sweat gland regeneration, burn wound healing, anhidrosis, hypohidrosis, transcription factor overexpression, sweat gland differentiation
MeSH terms
Epidermal Cells, Animals, Burns, Cell Differentiation, Hypohidrosis, Mice, Inbred C57BL, Repressor Proteins, Sweat Glands, Transfection, Wound Healing, Cell Transplantation, Cell Proliferation, Interferon Regulatory Factors, Mice, Forkhead Transcription Factors, Bone Morphogenetic Protein 5, Gene Knockdown Techniques, Transcriptome, Cellular Reprogramming

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