The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
CRISPR-Cas12a has both cis- and trans-cleavage activities on single-stranded DNA
Open accessAbstract
The CRISPR-associated protein Cas12a (previously known as Cpf1), which is an endonuclease from the type V-A CRISPR system, has been applied in both in vivo genome editing and in vitro DNA assembly. 1 , 2 , 3 Cas12a is guided by a single CRISPR RNA (crRNA) with a T-rich protospacer adjacent motif (PAM) sequence to cleave double-stranded DNA (dsDNA) targets, generating sticky ends. Different from Cas9, Cas12a cleaves both the target and non-target strands of a targeted dsDNA by a single active site in the RuvC catalytic pocket 4 , 5 , 6 (Supplementary information, Figure S12a ). Besides, Cas12a also processes precursor crRNAs to generate mature crRNAs. 7 However, the cleavage activity of Cas12a on single-stranded DNA (ssDNA) targets is less understood.
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Cites
Crystal structure of Cas9 in complex with guide RNA and target DNA
Nishimasu, H., Ran, F. A., Hsu, P. D., Konermann, S., Shehata, S. I., Dohmae, N., Ishitani, R., Zhang, F., & Nureki, O. (2014) · Cell · doi:10.1016/j.cell.2014.02.001 · Cited by 2,306
Cited by
Kaminski, M. M., Abudayyeh, O. O., Gootenberg, J. S., Zhang, F., & Collins, J. J. (2021) · Nature Biomedical Engineering · doi:10.1038/s41551-021-00760-7 · Cited by 1,259
CRISPR/Cas Systems towards Next-Generation Biosensing
Li, Y., Li, S., Wang, J., & Liu, G. (2019) · Trends in Biotechnology · doi:10.1016/j.tibtech.2018.12.005 · Cited by 917
Self-citationImproved genome editing by an engineered CRISPR-Cas12a
Ma, E., Chen, K., Shi, H., Stahl, E. C., Adler, B., Trinidad, M., Liu, J., Zhou, K., Ye, J., & Doudna, J. A. (2022) · Nucleic Acids Research · doi:10.1093/nar/gkac1192 · Cited by 110
Self-citationShen, Q., Ruan, H., Zhang, H., Wu, T., Zhu, K., Han, W., Dong, R., Ming, T., Qi, H., & Zhang, Y. (2024) · Frontiers in Microbiology · doi:10.3389/fmicb.2024.1375120 · Cited by 38
Wörle, E., Newman, A., D’Silva, J., Burgio, G., & Grohmann, D. (2022) · Nucleic Acids Research · doi:10.1093/nar/gkac767 · Cited by 32
Metadata
- Authors
- Li, S.-Y., Cheng, Q.-X., Liu, J.-K., Nie, X.-Q., Zhao, G.-P., & Wang, J.
- Venue
- Cell Research
- Published
- 2018-03-12
- Volume
- 28
- Issue
- 4
- Pages
- 491-493
- ISSN
- 1001-0602 (ISSN-L)
- 1748-7838
- OpenAlex
- W2790313560
- Cited by
- 1,130
- References
- 1
- FWCI
- 18.68
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Li, S.-Y., Cheng, Q.-X., Liu, J.-K., Nie, X.-Q., Zhao, G.-P., & Wang, J. (2018). CRISPR-Cas12a has both cis- and trans-cleavage activities on single-stranded DNA. Cell Research. https://doi.org/10.1038/s41422-018-0022-x
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