The DOI matches its Crossref record; 12 of the 12 references it cites were found in a registry or an index.
Engineered nanointerfaces for microfluidic isolation and molecular profiling of tumor-specific extracellular vesicles
Open accessAbstract
Extracellular vesicles (EVs) carry RNA, DNA, proteins, and lipids. Specifically, tumor-derived EVs have the potential to be utilized as disease-specific biomarkers. However, a lack of methods to isolate tumor-specific EVs has limited their use in clinical settings. Here we report a sensitive analytical microfluidic platform (EV HB-Chip) that enables tumor-specific EV-RNA isolation within 3 h. Using the EV HB-Chip, we achieve 94% tumor-EV specificity, a limit of detection of 100 EVs per μL, and a 10-fold increase in tumor RNA enrichment in comparison to other methods. Our approach allows for the subsequent release of captured tumor EVs, enabling downstream characterization and functional studies. Processing serum and plasma samples from glioblastoma multiforme (GBM) patients, we can detect the mutant EGFRvIII mRNA. Moreover, using next-generation RNA sequencing, we identify genes specific to GBM as well as transcripts that are hallmarks for the four genetic subtypes of the disease.
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Cites
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Cited by
Théry, C., Witwer, K. W., Aikawa, E., Alcaraz, M. J., Anderson, J. D., Andriantsitohaina, R., Antoniou, A., Arab, T., Archer, F., Atkin‐Smith, G. K., et al. (2018) · Journal of Extracellular Vesicles · doi:10.1080/20013078.2018.1535750 · Cited by 11,099
Self-citationRNA delivery by extracellular vesicles in mammalian cells and its applications
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Self-citationAdvances in therapeutic applications of extracellular vesicles
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Self-citationExosomes and Exosome-Inspired Vesicles for Targeted Drug Delivery
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Analysis of extracellular vesicles as emerging theranostic nanoplatforms
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Metadata
- Authors
- Reátegui, E., van der Vos, K. E., Lai, C. P., Zeinali, M., Atai, N. A., Aldikacti, B., Floyd, F. P., H. Khankhel, A., Thapar, V., Hochberg, F. H., Sequist, L. V., Nahed, B. V., S. Carter, B., Toner, M., Balaj, L., T. Ting, D., Breakefield, X. O., & Stott, S. L.
- Venue
- Nature Communications
- Published
- 2018-01-12
- Volume
- 9
- Issue
- 1
- Pages
- 175-175
- ISSN
- 2041-1723 (ISSN-L)
- OpenAlex
- W2783823932
- Cited by
- 330
- References
- 12
- FWCI
- 11.61
- Type
- journal article
- Language
- en
- Open access
- gold
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- Reátegui, E., van der Vos, K. E., Lai, C. P., Zeinali, M., Atai, N. A., Aldikacti, B., Floyd, F. P., H. Khankhel, A., Thapar, V., Hochberg, F. H., Sequist, L. V., Nahed, B. V., S. Carter, B., Toner, M., Balaj, L., T. Ting, D., Breakefield, X. O., & Stott, S. L. (2018). Engineered nanointerfaces for microfluidic isolation and molecular profiling of tumor-specific extracellular vesicles. Nature Communications. https://doi.org/10.1038/s41467-017-02261-1
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
