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Competition between microtubule-associated proteins directs motor transport

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Monroy, Brigette Y., Sawyer, Danielle L., Ackermann, Bryce E., Borden, Melissa M., Tan, Tracy C., Ori-McKenney, Kassandra M.·2018·Nature Communications
DOI 10.1038/s41467-018-03909-2PMC PMC5902456

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Abstract

Within cells, motor and non-motor microtubule-associated proteins (MAPs) simultaneously converge on the microtubule. How the binding activities of non-motor MAPs are coordinated and how they contribute to the balance and distribution of motor transport is unknown. Here, we examine the relationship between MAP7 and tau owing to their antagonistic roles in vivo. We find that MAP7 and tau compete for binding to microtubules, and determine a mechanism by which MAP7 displaces tau from the lattice. MAP7 promotes kinesin-based transport in vivo and strongly recruits kinesin-1 to the microtubule in vitro, providing evidence for direct enhancement of motor motility by a MAP. Both MAP7 and tau strongly inhibit kinesin-3 and have no effect on cytoplasmic dynein, demonstrating that MAPs differentially control distinct classes of motors. Overall, these results reveal a general principle for how MAP competition dictates access to the microtubule to determine the correct distribution and balance of motor activity.

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  • MAP7 competes with tau for lattice occupancy and has been shown to enhance kinesin-1 motility while inhibiting kinesin-3.

    “MAP7 competes with tau for lattice occupancy and has been shown to enhance kinesin-1 motility while inhibiting kinesin-3”

    From Tau hyperphosphorylation impairs cooperative binding to microtubules and perturbs organelle trafficking in neurons (2026) · cites this paper as Monroy et al., 2018

  • Envelope formation is sensitive to the intramolecular lattice spacing between α and β tubulin.

    “Envelope formation is sensitive to the intramolecular lattice spacing between α and β tubulin.”

    From Tau hyperphosphorylation impairs cooperative binding to microtubules and perturbs organelle trafficking in neurons (2026) · cites this paper as Monroy et al., 2018

    The Results section discusses MAP7 and tau competing for binding on the microtubule lattice, but does not address envelope formation or spacing between α- and β-tubulin. Searches for those terms and their paraphrases found no passage supporting the claim; page numbers were not available in our copy. (LLM, conf=0.76)

    Experimental — accuracy not yet measured

  • Tau does not form envelopes on GMPCPP-polymerized microtubules, but readily forms envelopes on compacted GDP-lattices or reversibly expanded taxol-lattices.

    “Tau does not form envelopes on GMPCPP-polymerized microtubules, which are irreversibly expanded, mimicking the GTP-state, but readily forms envelopes on compacted GDP-lattices or reversibly expanded taxol-lattices”

    From Tau hyperphosphorylation impairs cooperative binding to microtubules and perturbs organelle trafficking in neurons (2026) · cites this paper as Monroy et al., 2018

    The Results section (‘MAP7 and tau exhibit overlapping localization patterns’; page unavailable) reports tau binding to taxol-stabilized microtubules, but does not establish envelope formation. Searches for ‘envelopes,’ ‘GMPCPP,’ and compacted GDP lattices found no passage comparing envelope formation across those lattice states. (LLM, conf=0.76)

    Experimental — accuracy not yet measured

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Cited by

Sorted by citations

Authors
Monroy, B. Y., Sawyer, D. L., Ackermann, B. E., Borden, M. M., Tan, T. C., & Ori-McKenney, K. M.
Affiliations
University of California, Davis (US)
Corresponding author
Ori-McKenney, Kassandra M.
ORCID
Venue
Nature Communications
Publisher
Springer Science and Business Media LLC
Published
2018-04-16
Volume
9
Issue
1
Pages
1487-1487
ISSN
2041-1723 (ISSN-L)
DOI
10.1038/s41467-018-03909-2
PMC
PMC5902456
OpenAlex
W2953010568
Cited by
213
References
3
Citations by year
2026: 19 · 2025: 17 · 2024: 19 · 2023: 37 · 2022: 25 · 2021: 30 · 2020: 35 · 2019: 22 · 2018: 8 · 2017: 1
FWCI
13.13
Type
journal article
Language
en
Open access
Licence
Locations
  • Nature Communications (publishedVersion, cc-by)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nc-nd)
  • Europe PMC (PubMed Central) (publishedVersion, other-oa)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • RePEc: Research Papers in Economics (submittedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
  • eScholarship (California Digital Library) (submittedVersion)
  • eScholarship (California Digital Library) (submittedVersion, cc-by)
Funders
  • National Science Foundation (1650042)
  • March of Dimes Foundation
  • National Institutes of Health (1650042)
  • Eunice Kennedy Shriver National Institute of Child Health and Human Development (R00HD080981)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
  • Health Sciences › Medicine › Oncology › Cancer Treatment and Pharmacology
  • Health Sciences › Medicine › Physiology › Alzheimer's disease research and treatments
Keywords
microtubule-associated proteins, MAP7, tau protein, kinesin-1, kinesin-3, cytoplasmic dynein, road transport, microtubule lattice
MeSH terms
Animals, Binding, Competitive, Biological Transport, Drosophila melanogaster, Dyneins, Humans, Microtubule-Associated Proteins, Microtubules, Neurons, Protein Binding, Swine, Tubulin, Gene Expression, Kinesins, tau Proteins, Mice, Primary Cell Culture

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