Cited by 258Microtubule and mitosis dynamicsAdvanced Fluorescence Microscopy TechniquesCellular Mechanics and Interactions

Single-molecule investigation of the interference between kinesin, tau and MAP2c

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Seitz, Arne, Kojima, Hiroaki, Oiwa, Kazuhiro, Mandelkow, Eva-Maria, Song, Young-Hwa, Mandelkow, Eckhard·2002·The EMBO Journal

Abstract

Motor proteins and microtubule-associated proteins (MAPs) play important roles in cellular transport, regulation of shape and polarity of cells. While motor proteins generate motility, MAPs are thought to stabilize the microtubule tracks. However, the proteins also interfere with each other, such that MAPs are able to inhibit transport of vesicles and organelles in cells. In order to investigate the mechanism of MAP-motor interference in molecular detail, we have studied single kinesin molecules by total internal reflection fluorescence microscopy in the presence of different neuronal MAPs (tau, MAP2c). The parameters observed included run-length (a measure of processivity), velocity and frequency of attachment. The main effect of MAPs was to reduce the attachment frequency of motors. This effect was dependent on the concentration, the affinity to microtubules and the domain composition of MAPs. In contrast, once attached, the motors did not show a change in speed, nor in their run-length. The results suggest that MAPs can regulate motor activity on the level of initial attachment, but not during motion.

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Crossref · 10.1093/emboj/cdf503

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  • MAP2 has been shown to block the path of kinesin motors in in vitro assays.

    “MAP2 has been shown to block the path of kinesin motors in in vitro assays (46, 47, 48, 49, 50).”

    From Active transport by cytoplasmic dynein sustains dendritic MAP2 localization in developing neurons (2026) · cites this paper as [49]

    The Discussion says that MAPs, including MAP2c, are obstacles to kinesin attachment, not to its movement along a microtubule. The Abstract likewise reports reduced attachment frequency but no change in speed or run-length once a motor is attached. Thus this in vitro study does not show MAP2 blocking the path of moving kinesin motors. (LLM, conf=0.89)

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Authors
Seitz, A., Kojima, H., Oiwa, K., Mandelkow, E.-M., Song, Y.-H., & Mandelkow, E.
Affiliations
Max Planck Unit for Structural Molecular Biology (DE)
Corresponding author
Seitz, Arne
Venue
The EMBO Journal
Published
2002-09-16
Volume
21
Issue
18
Pages
4896-4905
ISSN
  • 0261-4189 (ISSN-L)
  • 1460-2075
OpenAlex
W1968991344
Cited by
257
References
49
Citations by year
2026: 3 · 2025: 12 · 2024: 4 · 2023: 5 · 2022: 5 · 2021: 8 · 2020: 8 · 2019: 12 · 2018: 7 · 2017: 15 · 2016: 11 · 2015: 12 · 2014: 18 · 2013: 10 · 2012: 12
FWCI
3.04
Type
journal article
Language
en
Open access
Locations
  • The EMBO Journal (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
  • Desy publication database (The Deutsches Elektronen-Synchrotron) (submittedVersion)
  • Max Planck Institute for Plasma Physics (submittedVersion)
  • Infoscience (Ecole Polytechnique Fédérale de Lausanne) (submittedVersion)
  • Infoscience (Ecole Polytechnique Fédérale de Lausanne) (submittedVersion, cc-by-nc-nd)
Funders
Deutsche Forschungsgemeinschaft
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Advanced Fluorescence Microscopy Techniques
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
Keywords
kinesin, tau protein, MAP2c, microtubule-associated proteins, microtubule stabilization, single-molecule tracking, total internal reflection fluorescence microscopy, run length, processivity, motor speed, neuronal transport
MeSH terms
Adenosine Triphosphatases, Animals, Biological Transport, Humans, Isoelectric Point, Microscopy, Fluorescence, Microtubule-Associated Proteins, Microtubules, Models, Biological, Protein Binding, Recombinant Proteins, Kinesins, tau Proteins, Genes, Reporter, Protein Isoforms, Molecular Motor Proteins, Rats

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