The DOI matches its Crossref record; 12 of the 12 references it cites were found in a registry or an index.
A low-cost paper-based synthetic biology platform for analyzing gut microbiota and host biomarkers
Open accessAbstract
There is a need for large-scale, longitudinal studies to determine the mechanisms by which the gut microbiome and its interactions with the host affect human health and disease. Current methods for profiling the microbiome typically utilize next-generation sequencing applications that are expensive, slow, and complex. Here, we present a synthetic biology platform for affordable, on-demand, and simple analysis of microbiome samples using RNA toehold switch sensors in paper-based, cell-free reactions. We demonstrate species-specific detection of mRNAs from 10 different bacteria that affect human health and four clinically relevant host biomarkers. We develop a method to quantify mRNA using our toehold sensors and validate our platform on clinical stool samples by comparison to RT-qPCR. We further highlight the potential clinical utility of the platform by showing that it can be used to rapidly and inexpensively detect toxin mRNA in the diagnosis of Clostridium difficile infections.
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The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments
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CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity
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Gut microbiota, metabolites and host immunity
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Gut bacteria that prevent growth impairments transmitted by microbiota from malnourished children
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Lin, K. N., Volkel, K., Tuck, J. M., & Keung, A. J. (2020) · Nature Communications · doi:10.1038/s41467-020-16797-2 · Cited by 117
Ayoubi‐Joshaghani, M. H., Dianat‐Moghadam, H., Seidi, K., Jahanban‐Esfahalan, A., Zare, P., & Jahanban‐Esfahlan, R. (2020) · Biotechnology and Bioengineering · doi:10.1002/bit.27248 · Cited by 52
Understanding the Functionality of Probiotics on the Edge of Artificial Intelligence (AI) Era
Asar, R., Erenler, S., Devecioglu, D., Ispirli, H., Karbancioglu-Guler, F., Ozturk, H. I., & Dertli, E. (2025) · Fermentation · doi:10.3390/fermentation11050259 · Cited by 37
Metadata
- Authors
- Takahashi, M. K., Tan, X., Dy, A. J., Braff, D., Akana, R. T., Furuta, Y., Donghia, N., Ananthakrishnan, A., & Collins, J. J.
- Venue
- Nature Communications
- Published
- 2018-08-21
- Volume
- 9
- Issue
- 1
- Pages
- 3347-3347
- ISSN
- 2041-1723 (ISSN-L)
- OpenAlex
- W2885265830
- Cited by
- 236
- References
- 12
- FWCI
- 11.61
- Type
- journal article
- Language
- en
- Open access
- gold
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- Takahashi, M. K., Tan, X., Dy, A. J., Braff, D., Akana, R. T., Furuta, Y., Donghia, N., Ananthakrishnan, A., & Collins, J. J. (2018). A low-cost paper-based synthetic biology platform for analyzing gut microbiota and host biomarkers. Nature Communications. https://doi.org/10.1038/s41467-018-05864-4
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
