The DOI matches its Crossref record; 8 of the 8 references it cites were found in a registry or an index.
A CRISPR–Cas9-triggered strand displacement amplification method for ultrasensitive DNA detection
Open accessAbstract
Although polymerase chain reaction (PCR) is the most widely used method for DNA amplification, the requirement of thermocycling limits its non-laboratory applications. Isothermal DNA amplification techniques are hence valuable for on-site diagnostic applications in place of traditional PCR. Here we describe a true isothermal approach for amplifying and detecting double-stranded DNA based on a CRISPR–Cas9-triggered nicking endonuclease-mediated Strand Displacement Amplification method (namely CRISDA). CRISDA takes advantage of the high sensitivity/specificity and unique conformational rearrangements of CRISPR effectors in recognizing the target DNA. In combination with a peptide nucleic acid (PNA) invasion-mediated endpoint measurement, the method exhibits attomolar sensitivity and single-nucleotide specificity in detection of various DNA targets under a complex sample background. Additionally, by integrating the technique with a Cas9-mediated target enrichment approach, CRISDA exhibits sub-attomolar sensitivity. In summary, CRISDA is a powerful isothermal tool for ultrasensitive and specific detection of nucleic acids in point-of-care diagnostics and field analyses.
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Cites
The new frontier of genome engineering with CRISPR-Cas9
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Genome-wide association study identifies novel breast cancer susceptibility loci
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Cited by
Kaminski, M. M., Abudayyeh, O. O., Gootenberg, J. S., Zhang, F., & Collins, J. J. (2021) · Nature Biomedical Engineering · doi:10.1038/s41551-021-00760-7 · Cited by 1,259
Point-of-care diagnostics for infectious diseases: From methods to devices
Wang, C., Liu, M., Wang, Z., Li, S., Deng, Y., & He, N. (2021) · Nano Today · doi:10.1016/j.nantod.2021.101092 · Cited by 622
Choi, J.-H., Lim, J., Shin, M., Paek, S.-H., & Choi, J.-W. (2021) · Nano Letters · doi:10.1021/acs.nanolett.0c04303 · Cited by 382
Zhu, H., Zhang, H., Xu, Y., Laššáková, S., Korabečná, M., & Neužil, P. (2020) · BioTechniques · doi:10.2144/btn-2020-0057 · Cited by 380
Feng, W., Newbigging, A. M., Tao, J., Cao, Y., Peng, H., Le, C., Wu, J., Pang, B., Li, J., Tyrrell, D. L., et al. (2021) · Chemical Science · doi:10.1039/d0sc06973f · Cited by 312
Metadata
- Authors
- Zhou, W., Hu, L., Ying, L., Zhao, Z., Chu, P. K., & Yu, X.-F.
- Venue
- Nature Communications
- Published
- 2018-11-27
- Volume
- 9
- Issue
- 1
- Pages
- 5012-5012
- ISSN
- 2041-1723 (ISSN-L)
- OpenAlex
- W2901552404
- Cited by
- 358
- References
- 8
- FWCI
- 9.02
- Type
- journal article
- Language
- en
- Open access
- gold
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- Zhou, W., Hu, L., Ying, L., Zhao, Z., Chu, P. K., & Yu, X.-F. (2018). A CRISPR–Cas9-triggered strand displacement amplification method for ultrasensitive DNA detection. Nature Communications. https://doi.org/10.1038/s41467-018-07324-5
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