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A small secreted protein triggers a TLR2/4-dependent inflammatory response during invasive Candida albicans infection

Wang, Wenjuan, Deng, Zihou, Wu, Hongyu, Zhao, Qun, Li, Tiantian, Zhu, Wencheng, Wang, Xiongjun, Tang, Longhai, Wang, Chengshu, Cui, Shu-Zhong, Xiao, Hui, Chen, Jiangye·2019·Nature Communications
DOI 10.1038/s41467-019-08950-3
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Abstract

Candida albicans can switch from commensal to pathogenic mode, causing mucosal or disseminated candidiasis. The host relies on pattern-recognition receptors including Toll-like receptors (TLRs) and C-type lectin receptors (CLRs) to sense invading fungal pathogens and launch immune defense mechanisms. However, the complex interplay between fungus and host innate immunity remains incompletely understood. Here we report that C . albicans upregulates expression of a small secreted cysteine-rich protein Sel1 upon encountering limited nitrogen and abundant serum. Sel1 activates NF-κB and MAPK signaling pathways, leading to expression of proinflammatory cytokines and chemokines. Comprehensive genetic and biochemical analyses reveal both TLR2 and TLR4 are required for the recognition of Sel1. Further, SEL1 -deficient C . albicans display an impaired immune response in vivo, causing increased morbidity and mortality in a bloodstream infection model. We identify a critical component in the Candida-host interaction that opens a new avenue to tackle Candida infection and inflammation.

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Metadata

Authors
Wang, W., Deng, Z., Wu, H., Zhao, Q., Li, T., Zhu, W., Wang, X., Tang, L., Wang, C., Cui, S.-Z., Xiao, H., & Chen, J.
Venue
Nature Communications
Publisher
Springer Science and Business Media LLC
Published
2019-03-04
Volume
10
Issue
1
ISSN
2041-1723
DOI
10.1038/s41467-019-08950-3
Cited by
71
References
7
Language
en
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