CoChat
Sign Up
Cited by 245Acute Myeloid Leukemia ResearchSingle-cell and spatial transcriptomicsCancer Genomics and Diagnostics

The DOI matches its Crossref record; 75 of the 76 references it cites were found in a registry or an index.

A general approach for detecting expressed mutations in AML cells using single cell RNA-sequencing

Open access
Petti, Allegra A., Williams, Stephen R., Miller, Christopher A., Fiddes, Ian T., Srivatsan, Sridhar N., Chen, David Y., Fronick, Catrina C., Fulton, Robert S., Church, Deanna M., Ley, Timothy J.·2019·Nature Communications
DOI 10.1038/s41467-019-11591-1PMC PMC6694122
Open this paper in CoChat — free accountOpen PDF

Abstract

Virtually all tumors are genetically heterogeneous, containing mutationally-defined subclonal cell populations that often have distinct phenotypes. Single-cell RNA-sequencing has revealed that a variety of tumors are also transcriptionally heterogeneous, but the relationship between expression heterogeneity and subclonal architecture is unclear. Here, we address this question in the context of Acute Myeloid Leukemia (AML) by integrating whole genome sequencing with single-cell RNA-sequencing (using the 10x Genomics Chromium Single Cell 5’ Gene Expression workflow). Applying this approach to five cryopreserved AML samples, we identify hundreds to thousands of cells containing tumor-specific mutations in each case, and use the results to distinguish AML cells (including normal-karyotype AML cells) from normal cells, identify expression signatures associated with subclonal mutations, and find cell surface markers that could be used to purify subclones for further study. This integrative approach for connecting genotype to phenotype is broadly applicable to any sample that is phenotypically and genetically heterogeneous.

Overview

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1038/s41467-019-11591-1

Cites

75 of 75 references verified0 citation errors · 0 not checkable

Statistics

Figures

Fig. 1 Workflow, coverage, and performance metrics for variant detection in single cells. a Cryopreserved bone marrow cells from AML patients underwent eWGS, bulk RNA-seq, and scRNA-seq. Somatic mutations were discovered using eWGS data, identified in individual cells using scRNA-seq data, and interpreted in the context of expression heterogeneity. b Fraction of unique transcripts (molecules) whose reads map to any given position up to 10 kbp away from the capture site in both the 5’ and 3’ kits. c Comparison of single-cell and bulk RNA-seq coverage data for specific genes of interest. d Relationship between RNA and eWGS VAF; dependence of Mutant Cell Fraction on eWGS VAF; dependence of Mutant Cell Detection Rate on bulk RNA VAF, and dependence of Mutant Cell Detection Rate on position of the mutation in the cDNA
Fig. 1 Workflow, coverage, and performance metrics for variant detection in single cells. a Cryopreserved bone marrow cells from AML patients underwent eWGS, bulk RNA-seq, and scRNA-seq. Somatic mutations were discovered using eWGS data, identified in individual cells using scRNA-seq data, and interpreted in the context of expression heterogeneity. b Fraction of unique transcripts (molecules) whose reads map to any given position up to 10 kbp away from the capture site in both the 5’ and 3’ kits. c Comparison of single-cell and bulk RNA-seq coverage data for specific genes of interest. d Relationship between RNA and eWGS VAF; dependence of Mutant Cell Fraction on eWGS VAF; dependence of Mutant Cell Detection Rate on bulk RNA VAF, and dependence of Mutant Cell Detection Rate on position of the mutation in the cDNACC BY · PubMed Central
Fig. 2 Clustering, overview of expression heterogeneity, and copy number analysis in 809653. a t-SNE projection of scRNA-seq data, with cells colored according to graph-based cluster assignment; putative AML clusters (based on later analyses) circled. b Hierarchical clustering of the most heavily weighted genes in each principal component, averaged within graph-based clusters. Each column represents a cluster from panel a. c CNV analysis: blue, cells with detected CNVs; gray, no detected CNVs
Fig. 2 Clustering, overview of expression heterogeneity, and copy number analysis in 809653. a t-SNE projection of scRNA-seq data, with cells colored according to graph-based cluster assignment; putative AML clusters (based on later analyses) circled. b Hierarchical clustering of the most heavily weighted genes in each principal component, averaged within graph-based clusters. Each column represents a cluster from panel a. c CNV analysis: blue, cells with detected CNVs; gray, no detected CNVsCC BY · PubMed Central
Fig. 3 Single-cell mutation detection and interpretation in case 809653. a Clonality inferred from eWGS, with subclonal driver genes labeled. b t-SNE projection of scRNA-seq data with cells colored according to graph-based cluster assignment. In panels b–g, putative clusters of AML cells are circled. c Cells colored according to inferred lineage; RBC = red blood cell, HSC = hematopoietic stem cell, MEP = myeloid-erythroid progenitor, MD = myeloid dendritic cell. d Cells colored according to cell cycle phase. e–g Cells colored according to single-cell genotype at the TP53E286G, CEBPAR142fs, and NRASG12D sites: blue, at least one mutant read detected; yellow, wild-type reads only; gray, no coverage. h Cells colored according to single-cell genotype at the homozygous BAG1 germline SNP: blue, at least one mutant read detected; gray, no coverage
Fig. 3 Single-cell mutation detection and interpretation in case 809653. a Clonality inferred from eWGS, with subclonal driver genes labeled. b t-SNE projection of scRNA-seq data with cells colored according to graph-based cluster assignment. In panels b–g, putative clusters of AML cells are circled. c Cells colored according to inferred lineage; RBC = red blood cell, HSC = hematopoietic stem cell, MEP = myeloid-erythroid progenitor, MD = myeloid dendritic cell. d Cells colored according to cell cycle phase. e–g Cells colored according to single-cell genotype at the TP53E286G, CEBPAR142fs, and NRASG12D sites: blue, at least one mutant read detected; yellow, wild-type reads only; gray, no coverage. h Cells colored according to single-cell genotype at the homozygous BAG1 germline SNP: blue, at least one mutant read detected; gray, no coverageCC BY · PubMed Central
Fig. 4 Single-cell mutation detection and interpretation in additional cases ordered by the differentiation signature of AML cells. a 721214, top to bottom: clonality inferred from eWGS; cells colored according to closest inferred lineage (RBC = red blood cell, HSC = hematopoietic stem cell, CMP = common myeloid progenitor); cells colored according to cell cycle phase; cells colored according to single-cell genotype at the indicated site: blue, at least one mutant read detected; yellow, wild-type reads only; gray, no coverage. b 548327, putative AML cells circled. c 508084. d 782328
Fig. 4 Single-cell mutation detection and interpretation in additional cases ordered by the differentiation signature of AML cells. a 721214, top to bottom: clonality inferred from eWGS; cells colored according to closest inferred lineage (RBC = red blood cell, HSC = hematopoietic stem cell, CMP = common myeloid progenitor); cells colored according to cell cycle phase; cells colored according to single-cell genotype at the indicated site: blue, at least one mutant read detected; yellow, wild-type reads only; gray, no coverage. b 548327, putative AML cells circled. c 508084. d 782328CC BY · PubMed Central
Fig. 5 GATA2R361C Subclonal expression signature. a t-SNE projection showing mutation-expressing cells in blue (GATA2R361C) and pink (TIMM17BL122fs). b Cells colored according to graph-based cluster assignment. c Heatmap of top 50 mutation-dependent genes, with bar graph showing mutant cell fraction in each AML cluster (labeled to the right of the heatmap). d Cells colored according to VIM expression (left), and scatterplot showing average VIM expression in each cluster as a function of the subclonal mutation fraction of each cluster (right). e t-SNE plot constructed from mutant cells, which are colored according to the mutation they contain: GATA2R361C, yellow; DNMT3AR882H, pink; FLT3-ITD, green; FLT3F612L, purple; NPM1W288FS; other somatic mutation(s), gray. f Mutant cells colored according to graph-based cluster. g Heatmap of top 25 subclonal mutation-dependent genes, with bar graph showing mutant cell fraction in each cluster (labeled to the right of the heatmap). Genes that are highly correlated with VIM in TCGA are indicated with blue dots. h Venn diagram indicating gene sets used to identify the VIM regulome
Fig. 5 GATA2R361C Subclonal expression signature. a t-SNE projection showing mutation-expressing cells in blue (GATA2R361C) and pink (TIMM17BL122fs). b Cells colored according to graph-based cluster assignment. c Heatmap of top 50 mutation-dependent genes, with bar graph showing mutant cell fraction in each AML cluster (labeled to the right of the heatmap). d Cells colored according to VIM expression (left), and scatterplot showing average VIM expression in each cluster as a function of the subclonal mutation fraction of each cluster (right). e t-SNE plot constructed from mutant cells, which are colored according to the mutation they contain: GATA2R361C, yellow; DNMT3AR882H, pink; FLT3-ITD, green; FLT3F612L, purple; NPM1W288FS; other somatic mutation(s), gray. f Mutant cells colored according to graph-based cluster. g Heatmap of top 25 subclonal mutation-dependent genes, with bar graph showing mutant cell fraction in each cluster (labeled to the right of the heatmap). Genes that are highly correlated with VIM in TCGA are indicated with blue dots. h Venn diagram indicating gene sets used to identify the VIM regulomeCC BY · PubMed Central
Fig. 6 Orthogonal confirmation of GATA2R361C expression signature by flow cytometry and targeted sequencing. a Cells colored according to CD99 expression (top), and scatterplot showing average CD99 expression in each cluster as a function of the GATA2 mutation fraction of each cluster (bottom). b Sorting strategy: Cells staining positive for the dead cell dye Sytox-blue were excluded, then debris was excluded. Singlets were gated for final sorting of CD99-low and CD99-high expressing populations. c Gating of cells based on CD99 expression using flow cytometry (bone marrow, left; peripheral blood, right). d Variant allele fraction of the founding clone DNMT3AR882H mutation and the subclonal GATA2R361C mutation in unsorted cells (gray), CD99-high cells (blue), and CD99-low cells (red) (bone marrow, left; peripheral blood, right)
Fig. 6 Orthogonal confirmation of GATA2R361C expression signature by flow cytometry and targeted sequencing. a Cells colored according to CD99 expression (top), and scatterplot showing average CD99 expression in each cluster as a function of the GATA2 mutation fraction of each cluster (bottom). b Sorting strategy: Cells staining positive for the dead cell dye Sytox-blue were excluded, then debris was excluded. Singlets were gated for final sorting of CD99-low and CD99-high expressing populations. c Gating of cells based on CD99 expression using flow cytometry (bone marrow, left; peripheral blood, right). d Variant allele fraction of the founding clone DNMT3AR882H mutation and the subclonal GATA2R361C mutation in unsorted cells (gray), CD99-high cells (blue), and CD99-low cells (red) (bone marrow, left; peripheral blood, right)CC BY · PubMed Central
Fig. 7 Additional mutation-associated gene expression signatures. a t-SNE plot of 508084, cells colored by graph-based cluster. b Cells colored according to coverage at the RNF10NULL site (mutant = blue, wild-type = yellow). c Genes that are differentially expressed between mutant-rich and mutant-poor clusters. d t-SNE plot of 809653, cells colored by graph-based cluster. e Cells colored according to coverage at the CEBPAR142fs site (mutant = blue, wild-type = yellow). f Genes that are differentially expressed between mutant-rich and mutant-poor clusters; non-AML cells shown for comparison
Fig. 7 Additional mutation-associated gene expression signatures. a t-SNE plot of 508084, cells colored by graph-based cluster. b Cells colored according to coverage at the RNF10NULL site (mutant = blue, wild-type = yellow). c Genes that are differentially expressed between mutant-rich and mutant-poor clusters. d t-SNE plot of 809653, cells colored by graph-based cluster. e Cells colored according to coverage at the CEBPAR142fs site (mutant = blue, wild-type = yellow). f Genes that are differentially expressed between mutant-rich and mutant-poor clusters; non-AML cells shown for comparisonCC BY · PubMed Central

Verification

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Citations

Cites

Show all 76 references

Research works printed but not linked: 1

  1. The Cancer Genome Atlas Research Network. Genomic and epigenomic landscapes of adult de novo acute myeloid leukemia. N. Engl. J. Med. 368, 2059–2074 (2013). · DOI · Paper page

Cited by

Sorted by citations

Related

See all

Metadata

Authors
Petti, A. A., Williams, S. R., Miller, C. A., Fiddes, I. T., Srivatsan, S. N., Chen, D. Y., Fronick, C. C., Fulton, R. S., Church, D. M., & Ley, T. J.
Affiliations
  • Washington University in St. Louis (US)
  • 10X Genomics (United States) (US)
Corresponding author
Ley, Timothy J.
ORCID
Venue
Nature Communications
Publisher
Springer Science and Business Media LLC
Published
2019-08-14
Volume
10
Issue
1
Pages
3660-3660
ISSN
2041-1723 (ISSN-L)
DOI
10.1038/s41467-019-11591-1
PMC
PMC6694122
OpenAlex
W2968098426
Cited by
215
References
76
Citations by year
2026: 14 · 2025: 26 · 2024: 35 · 2023: 44 · 2022: 32 · 2021: 40 · 2020: 22 · 2019: 2
FWCI
17.12
Type
journal article
Language
en
Update notices
correction (2022-07-21)
Open access
Licence
Locations
  • Nature Communications (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • Digital Commons@Becker (Washington University School of Medicine) (submittedVersion, cc-by)
  • RePEc: Research Papers in Economics (submittedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
U.S. Department of Health & Human Services | NIH | National Cancer Institute (CA101937, CA197561, CA167540, CA211782, K12CA167540, R50CA211782)
Topics
  • Health Sciences › Medicine › Hematology › Acute Myeloid Leukemia Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Single-cell and spatial transcriptomics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Cancer Genomics and Diagnostics
Keywords
acute myeloid leukemia, subclonal heterogeneity, cancer mutations, transcriptional heterogeneity, single-cell RNA sequencing, genotype-phenotype correlation, cell surface markers, normal karyotype AML, tumor cell identification
MeSH terms
Whole Genome Sequencing, Genotype, Humans, Mutation, Phenotype, RNA, Leukemia, Myeloid, Acute, Sequence Analysis, RNA, Polymorphism, Single Nucleotide, Gene Expression Profiling, Genomics, Single-Cell Analysis

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review