The DOI matches its Crossref record; 61 of the 61 references it cites were found in a registry or an index.
Regulation of reactive oxygen species during plant immunity through phosphorylation and ubiquitination of RBOHD
Open accessAbstract
Production of reactive oxygen species (ROS) is critical for successful activation of immune responses against pathogen infection. The plant NADPH oxidase RBOHD is a primary player in ROS production during innate immunity. However, how RBOHD is negatively regulated remains elusive. Here we show that RBOHD is regulated by C-terminal phosphorylation and ubiquitination. Genetic and biochemical analyses reveal that the PBL13 receptor-like cytoplasmic kinase phosphorylates RBOHD’s C-terminus and two phosphorylated residues (S862 and T912) affect RBOHD activity and stability, respectively. Using protein array technology, we identified an E3 ubiquitin ligase PIRE (PBL13 interacting RING domain E3 ligase) that interacts with both PBL13 and RBOHD. Mimicking phosphorylation of RBOHD (T912D) results in enhanced ubiquitination and decreased protein abundance. PIRE and PBL13 mutants display higher RBOHD protein accumulation, increased ROS production, and are more resistant to bacterial infection. Thus, our study reveals an intricate post-translational network that negatively regulates the abundance of a conserved NADPH oxidase.
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Self-citationA flagellin-induced complex of the receptor FLS2 and BAK1 initiates plant defence
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PTI-ETI crosstalk: an integrative view of plant immunity
Yuan, M., Ngou, B. P. M., Ding, P., & Xin, X.-F. (2021) · Current Opinion in Plant Biology · doi:10.1016/j.pbi.2021.102030 · Cited by 915
Nanobiotechnology-based strategies for enhanced crop stress resilience
Zhao, L., Bai, T., Wei, H., Gardea-Torresdey, J. L., Keller, A., & White, J. C. (2022) · Nature Food · doi:10.1038/s43016-022-00596-7 · Cited by 270
Metadata
- Authors
- Lee, D., Lal, N. K., Lin, Z.-J. D., Ma, S., Liu, J., Castro, B., Toruño, T., Dinesh-Kumar, S. P., & Coaker, G.
- Venue
- Nature Communications
- Published
- 2020-04-15
- Volume
- 11
- Issue
- 1
- Pages
- 1838-1838
- ISSN
- 2041-1723 (ISSN-L)
- OpenAlex
- W3017359334
- Cited by
- 319
- References
- 61
- FWCI
- 20.24
- Type
- journal article
- Language
- en
- Open access
- gold
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- Lee, D., Lal, N. K., Lin, Z.-J. D., Ma, S., Liu, J., Castro, B., Toruño, T., Dinesh-Kumar, S. P., & Coaker, G. (2020). Regulation of reactive oxygen species during plant immunity through phosphorylation and ubiquitination of RBOHD. Nature Communications. https://doi.org/10.1038/s41467-020-15601-5
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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