The DOI matches its Crossref record; 11 of the 11 references it cites were found in a registry or an index.
An alpaca nanobody neutralizes SARS-CoV-2 by blocking receptor interaction
Open accessAbstract
SARS-CoV-2 enters host cells through an interaction between the spike glycoprotein and the angiotensin converting enzyme 2 (ACE2) receptor. Directly preventing this interaction presents an attractive possibility for suppressing SARS-CoV-2 replication. Here, we report the isolation and characterization of an alpaca-derived single domain antibody fragment, Ty1, that specifically targets the receptor binding domain (RBD) of the SARS-CoV-2 spike, directly preventing ACE2 engagement. Ty1 binds the RBD with high affinity, occluding ACE2. A cryo-electron microscopy structure of the bound complex at 2.9 Å resolution reveals that Ty1 binds to an epitope on the RBD accessible in both the ‘up’ and ‘down’ conformations, sterically hindering RBD-ACE2 binding. While fusion to an Fc domain renders Ty1 extremely potent, Ty1 neutralizes SARS-CoV-2 spike pseudovirus as a 12.8 kDa nanobody, which can be expressed in high quantities in bacteria, presenting opportunities for manufacturing at scale. Ty1 is therefore an excellent candidate as an intervention against COVID-19.
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Animal immunization, in vitro display technologies, and machine learning for antibody discovery
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The Virtual Lab of AI agents designs new SARS-CoV-2 nanobodies
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(2022) · doi:10.1016/j.chempr.2022.07.012 · Cited by 64
The Virtual Lab: AI Agents Design New SARS-CoV-2 Nanobodies with Experimental Validation
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Metadata
- Authors
- Hanke, L., Vidakovics Perez, L., Sheward, D. J., Das, H., Schulte, T., Moliner-Morro, A., Corcoran, M., Achour, A., Karlsson Hedestam, G. B., Hällberg, B. M., Murrell, B., & McInerney, G. M.
- Venue
- Nature Communications
- Published
- 2020-09-04
- Volume
- 11
- Issue
- 1
- Pages
- 4420-4420
- ISSN
- 2041-1723 (ISSN-L)
- OpenAlex
- W3081714253
- Cited by
- 343
- References
- 11
- FWCI
- 7.70
- Type
- journal article
- Language
- en
- Open access
- gold
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- Hanke, L., Vidakovics Perez, L., Sheward, D. J., Das, H., Schulte, T., Moliner-Morro, A., Corcoran, M., Achour, A., Karlsson Hedestam, G. B., Hällberg, B. M., Murrell, B., & McInerney, G. M. (2020). An alpaca nanobody neutralizes SARS-CoV-2 by blocking receptor interaction. Nature Communications. https://doi.org/10.1038/s41467-020-18174-5
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